The Glycoconjugates

The Glycoconjugates

Mammalian Glycoproteins and Glycolipids

1st Edition - January 1, 1977

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  • Editor: Martin Horowitz
  • eBook ISBN: 9780323145398

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The Glycoconjugates: Mammalian Glycoproteins and Glycolipids, Volume I is a collaboration of different experts in the field of molecular biology on the subject of glycoconjugates. The text discusses the historical aspect, nomenclature, biosynthesis, and catabolism of glycoconjugates and their role in normal and pathological processes. Also covered are topics such as the purification of glycoproteins; the elucidation of the amino acid sequence of glycoproteins; the glycoproteins found in the different parts of the human body; glycoenzymes; and glycohormones. The book is recommended for molecular biologists, organic chemists, and biochemists who would like to know more about glycolipids and glycoproteins and their applications.

Table of Contents

  • List of Contributors


    Contents of Volume II

    1 General Aspects

    I. Historical

    II. Nomenclature

    III. The Sialic Acids

    IV. Biosynthesis and Catabolism

    V. Plasma Membranes and Mucopolysaccharides

    VI. Role in Normal and Pathological Processes


    2 Methods of Analysis and Structural Determination

    Section 1 Purification of Glycoproteins and Criteria of Purity

    I. Isolation and Purification of Glycoproteins

    II. The Concept and Proof of Homogeneity


    Section 2 Histochemistry

    I. Introduction

    II. Staining Methods and Their Specificity

    III. Electron Microscopic Techniques

    IV. Radioautography

    V. Fluorescence Techniques

    VI. Quantitation


    Section 3 Use of Enzymes in Elucidation of Structure

    I. Introduction

    II. Methods and Procedures

    III. Structural Studies on Glycoproteins

    IV. Structural Studies on Sphingoglycolipids

    V. Concluding Remarks


    Section 4 Nature of Linkages between Protein Core and Oligosaccharides

    I. Introduction

    II. N-Acetylglucosaminylasparagine Linkage

    III. N-Acetylgalactosaminylserine (-Threonine) Linkage

    IV. Galactosylhydroxylysine Linkage

    V. Addendum: Further Discussion of the β-Elimination Reaction (by Ward Pigman and Fred Downs)

    Procedure (Downs and Pigman, 1976)


    Section 5 The Elucidation of the Amino Acid Sequence of Proteins and Glycoproteins

    I. Introduction

    II. Considerations Preceding the Elucidation of Amino Acid Sequence

    III. Elucidation of Amino Acid Sequence

    IV. Determination of the Positions of Disulfide Bonds

    V. Additional Aspects of the Study of the Primary Structure Pertaining Particularly to Glycoproteins

    VI. Protein Variants

    VII. Addendum


    Section 6 Molecular Weight Determination and Physical Properties

    I. Molecular Parameters

    II. Methods Used for Determination of Molecular Parameters


    3 Mucus Glycoproteins

    Section 1 Introduction

    I. Sources

    II. Structure

    III. Blood Group Activity

    IV. Stimulatory Processes

    V. Comparison of Mucus Glycoproteins


    Section 2 Submandibular and Sublingual Glycoproteins

    I. Characterized Products

    II. Preparation

    III. Polydispersity and Molecular Weight

    IV. Composition of Some Purified Salivary Glycoproteins

    V. Structure of Protein Core


    Section 3 Human Submaxillary, Sublingual, and Parotid Glycoproteins and Enamel Pellicle

    I. Introduction

    II. Methods of Collection of Saliva

    III. General Methods of Identification, Separation, and Characterization

    IV. Glycoproteins of Submaxillary Saliva

    V. Glycoproteins of Sublingual Saliva

    VI. Glycoproteins of Parotid Saliva

    VII. Glycoproteins of Whole Saliva

    VIII. Factors Affecting Composition of Saliva

    IX. Enamel Pellicles


    Section 4 Blood Group Glycoproteins

    I. Introduction

    II. Preparation

    III. Composition

    IV. Oligosaccharide Composition

    V. Present Status


    Section 5 Gastrointestinal Glycoproteins

    I. Introduction

    II. Stomach

    III. Bile Mucins

    IV. Intestinal Mucins

    V. Addendum: Histochemistry


    Section 6 Respiratory Tract

    I. Introduction

    II. Collection, Purification, and Structure

    III. Secretion

    IV. Relation to Human Disease

    V. Summary


    Section 7 Cervical Mucus Glycoproteins

    I. Functional Role of Glycoproteins in Cervical Mucus

    II. Hormonal Regulation

    III. Physical Properties and Structure of the Glycoproteins

    IV. Mechanisms of Action of Sex Hormones

    V. Purification

    VI. Methods of Preparation and Purification

    VII. Analysis of Purified Fractions

    VIII. Pathology


    4 Blood Glycoproteins

    Section 1 Plasma

    I. Introduction

    II. Description of the Well-Characterized Plasma Glycoproteins

    III. Chemical and Physicochemical Data on Well-Characterized Plasma Glycoproteins


    Section 2 Immunoglobulins

    I. Introduction

    II. Classification of Immunoglobulins

    III. The Variable Regions and the Combining Site

    IV. Genetic Variation

    V. The Hinge Region and Disulfide Bonding of Subunits

    VI. Immunoglobulin Evolution

    VII. Antibody Diversity

    VIII. Carbohydrate on Immunoglobulins


    5 Glycoproteins of other Specific Systems

    Section 1 Glycoproteins and Glycolipids of the Nervous System

    I. Introduction

    II. Brain Glycoproteins

    III. Brain Glycolipids

    IV. Metabolism of Brain Glycoconjugates

    V. The Functional Roles of Brain Glycoconjugates

    VI. Glycoconjugates in Relation to Functional Stimulation, Learning, and Memory

    VII. Conclusions and Perspectives


    Section 2 Glycoenzymes and Glycohormones

    I. Introduction

    II. General

    III. Glycoenzymes

    IV. Glycohormones

    V. Role of Carbohydrate


    Section 3 Milk Glycoproteins and Oligosaccharides

    I. Introduction

    II. Glycoproteins and Glycopeptides

    III. Oligosaccharides


    Section 4 Urinary Glycoproteins, Glycopeptides, and Oligosaccharides

    I. Introduction

    II. Glycoproteins and Glycopeptides in Normal and Pathological Urine

    III. Oligosaccharides in Normal and Pathological Urine

    IV. Concluding Remarks


    6 Chemistry of Mammalian Glycolipids

    I. Introduction

    II. Nomenclature

    III. Methods of Isolation and Characterization of Glycosphingolipids

    IV. History and Occurrence of Glycolipids

    V. Distribution of Glycosphingolipids and Glycoglycerolipids



Product details

  • No. of pages: 578
  • Language: English
  • Copyright: © Academic Press 1977
  • Published: January 1, 1977
  • Imprint: Academic Press
  • eBook ISBN: 9780323145398

About the Editor

Martin Horowitz

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