The Cell

The Cell

Biochemistry, Physiology, Morphology

1st Edition - January 1, 1959

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  • Editors: Jean Brachet, Alfred E. Mirsky
  • eBook ISBN: 9781483216546

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Description

The Cell: Biochemistry, Physiology, Morphology, Volume I focuses on the advancements of processes, techniques, and approaches used in studies on the structure and functions of cells. The selection first offers information on the optical methods in cytology, fixation and staining, and autoradiography. Discussions focus on microscopical analysis, techniques related to autoradiography, staining for electron microscopy and light microscopy, fixation, electron microscope in cytology, and X-ray microscopy of cells. The text then takes a look at the quantitative microscopical techniques for single cells and quantitative microchemical techniques of histo- and cytochemistry. The manuscript ponders on micrurgical studies on living cells, isolation of subcellular components, and "tissue culture," cellular autonomy, and cellular interrelations. Topics include standardization procedures, factors influencing the choice of isolation procedure, future applications and extensions of micrurgy, and applications of micrurgy to the study of living cells. The effects of radiations on cells, acquisition of biological specificity, and nucleocytoplasmic interactions in eggs and embryos are also discussed. The selection is a dependable reference for researchers interested in pursuing further studies on the biochemistry, physiology, and morphology of cells.

Table of Contents


  • List of Contributors

    Preface

    Contents, Volumes II AND III

    Contents, Volumes IV AND V

    Introduction

    Part I: Methods

    1. Optical Methods in Cytology

    I. Introduction

    II. Extensions of Optical Microscopy

    III. The Electron Microscope in Cytology

    IV. The X-Ray Microscopy of Cells

    V. Conclusion

    References

    2. Fixation and Staining

    I. Definition of Fixation and Staining

    II. Fixation

    III. Staining for Light Microscopy

    IV. Staining for Electron Microscopy

    References

    3. Autoradiography

    I. Introduction

    II. Outline of the Various Autoradiography Techniques

    III. Techniques Related to Autoradiography

    IV. Microscopical Analysis

    V. Conclusion

    References

    4. Quantitative Microscopical Techniques for Single Cells

    I. Introduction

    II. Methods

    III. Individual Techniques

    IV. Conclusions

    References

    5. Quantitative Microchemical Techniques of Histo- and Cytochemistry

    I. Introduction

    II. Preparation of Samples

    III. Measurement of Amount of Sample

    IV. Titrimetric Techniques

    V. Spectrophotometric Techniques

    VI. Flame Photometric Techniques

    VII. Fluorometric Techniques

    VIII. Gasometric Techniques

    IX. Dilatometric Techniques

    X. Microbiological Techniques

    XI. Conclusions

    References

    6. Micrurgical Studies on Living Cells

    I. Historical

    II. Instrumentation and Procedures

    III. Application of Micrurgy to the Study of Living Cells

    IV. Subcellular Transplantation

    V. Future Applications and Extensions of Micrurgy

    References

    7. The Isolation of Subcellular Components

    I. Introduction

    II. Factors Influencing the Choice of Isolation Procedure

    III. The Techniques of Cell Fractionation

    IV. Some Representative Isolation Procedures

    V. Standardization Procedures

    VI. Some General Conclusions

    References

    8. The Cell as Organism. "Tissue Culture," Cellular Autonomy, and Cellular Interrelations

    I. Introduction

    II. History

    III. The Subject Matter

    IV. Methods

    V. Conclusions

    References

    Part II: Problems of Cell Biology

    9. Fertilization

    I. General Aspects of Fertilization

    II. Fertilization in Selected Cases

    III. Conclusions

    References

    10. Sex Determination

    I. Introduction

    II. Cytogenetic Theory of Sex Determination

    III. Karyologic Basis of the Mechanisms of Sex Determination

    IV. Experimental Verifications of the Cytogenetic Theory of Sex Determination

    V. Genetic Bipotentiality of Sex

    VI. Intersexuality and Genetic Interpretation of Sex: Genic Balance

    VII. Epigamous Factors, Intersexuality and Sex Determination

    VIII. Conclusions

    References

    11. Differentiation of Vertebrate Cells

    I. The Concept of Differentiation

    II. Criteria of Differentiation

    III. Properties of Cytodifferentiation

    IV. Theoretical Considerations

    References

    12. Patterns of Cell Growth and Differentiation in Plants

    I. Introduction

    II. Shoot Development from a Single Apical Cell

    III. Apical Development from a Group of Initial Cells

    References

    13. Nucleocytoplasmic Interactions in Eggs and Embryos

    I. Introduction

    II. Organization of the Egg

    III. Nucleocytoplasmic Interactions in Cleavage

    IV. Nucleocytoplasmic Interactions in Differentiation

    V. General Summary

    References

    14. The Acquisition of Biological Specificity

    I. Biological Specificity

    II. Transplantation Immunity

    III. The Ontogeny of Transplantation Immunity

    IV. Actively Acquired Tolerance

    V. Development of Tissue Specificity

    VI. Other Responses of Developing Systems to Implantation of Adult Tissues

    VII. Concluding Remarks; Perspectives

    References

    15. Effects of Radiations on Cells

    I. Introduction

    II. Action of Radiations at the Molecular Level

    III. Biochemical Effects

    IV. Cytochemical Effects

    V. Biological Effects

    VI. Variables in Radiation Effects

    VII. Alterations of Radiation Effects by Foreign Agents

    References

    Author Index

    Subject Index

Product details

  • No. of pages: 840
  • Language: English
  • Copyright: © Academic Press 1959
  • Published: January 1, 1959
  • Imprint: Academic Press
  • eBook ISBN: 9781483216546

About the Editors

Jean Brachet

Alfred E. Mirsky

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