The Cell - 1st Edition - ISBN: 9780121233013, 9781483216546

The Cell

1st Edition

Biochemistry, Physiology, Morphology

Editors: Jean Brachet Alfred E. Mirsky
eBook ISBN: 9781483216546
Imprint: Academic Press
Published Date: 1st January 1959
Page Count: 840
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The Cell: Biochemistry, Physiology, Morphology, Volume I focuses on the advancements of processes, techniques, and approaches used in studies on the structure and functions of cells.

The selection first offers information on the optical methods in cytology, fixation and staining, and autoradiography. Discussions focus on microscopical analysis, techniques related to autoradiography, staining for electron microscopy and light microscopy, fixation, electron microscope in cytology, and X-ray microscopy of cells. The text then takes a look at the quantitative microscopical techniques for single cells and quantitative microchemical techniques of histo- and cytochemistry.

The manuscript ponders on micrurgical studies on living cells, isolation of subcellular components, and "tissue culture," cellular autonomy, and cellular interrelations. Topics include standardization procedures, factors influencing the choice of isolation procedure, future applications and extensions of micrurgy, and applications of micrurgy to the study of living cells. The effects of radiations on cells, acquisition of biological specificity, and nucleocytoplasmic interactions in eggs and embryos are also discussed.

The selection is a dependable reference for researchers interested in pursuing further studies on the biochemistry, physiology, and morphology of cells.

Table of Contents

List of Contributors


Contents, Volumes II AND III

Contents, Volumes IV AND V


Part I: Methods

1. Optical Methods in Cytology

I. Introduction

II. Extensions of Optical Microscopy

III. The Electron Microscope in Cytology

IV. The X-Ray Microscopy of Cells

V. Conclusion


2. Fixation and Staining

I. Definition of Fixation and Staining

II. Fixation

III. Staining for Light Microscopy

IV. Staining for Electron Microscopy


3. Autoradiography

I. Introduction

II. Outline of the Various Autoradiography Techniques

III. Techniques Related to Autoradiography

IV. Microscopical Analysis

V. Conclusion


4. Quantitative Microscopical Techniques for Single Cells

I. Introduction

II. Methods

III. Individual Techniques

IV. Conclusions


5. Quantitative Microchemical Techniques of Histo- and Cytochemistry

I. Introduction

II. Preparation of Samples

III. Measurement of Amount of Sample

IV. Titrimetric Techniques

V. Spectrophotometric Techniques

VI. Flame Photometric Techniques

VII. Fluorometric Techniques

VIII. Gasometric Techniques

IX. Dilatometric Techniques

X. Microbiological Techniques

XI. Conclusions


6. Micrurgical Studies on Living Cells

I. Historical

II. Instrumentation and Procedures

III. Application of Micrurgy to the Study of Living Cells

IV. Subcellular Transplantation

V. Future Applications and Extensions of Micrurgy


7. The Isolation of Subcellular Components

I. Introduction

II. Factors Influencing the Choice of Isolation Procedure

III. The Techniques of Cell Fractionation

IV. Some Representative Isolation Procedures

V. Standardization Procedures

VI. Some General Conclusions


8. The Cell as Organism. "Tissue Culture," Cellular Autonomy, and Cellular Interrelations

I. Introduction

II. History

III. The Subject Matter

IV. Methods

V. Conclusions


Part II: Problems of Cell Biology

9. Fertilization

I. General Aspects of Fertilization

II. Fertilization in Selected Cases

III. Conclusions


10. Sex Determination

I. Introduction

II. Cytogenetic Theory of Sex Determination

III. Karyologic Basis of the Mechanisms of Sex Determination

IV. Experimental Verifications of the Cytogenetic Theory of Sex Determination

V. Genetic Bipotentiality of Sex

VI. Intersexuality and Genetic Interpretation of Sex: Genic Balance

VII. Epigamous Factors, Intersexuality and Sex Determination

VIII. Conclusions


11. Differentiation of Vertebrate Cells

I. The Concept of Differentiation

II. Criteria of Differentiation

III. Properties of Cytodifferentiation

IV. Theoretical Considerations


12. Patterns of Cell Growth and Differentiation in Plants

I. Introduction

II. Shoot Development from a Single Apical Cell

III. Apical Development from a Group of Initial Cells


13. Nucleocytoplasmic Interactions in Eggs and Embryos

I. Introduction

II. Organization of the Egg

III. Nucleocytoplasmic Interactions in Cleavage

IV. Nucleocytoplasmic Interactions in Differentiation

V. General Summary


14. The Acquisition of Biological Specificity

I. Biological Specificity

II. Transplantation Immunity

III. The Ontogeny of Transplantation Immunity

IV. Actively Acquired Tolerance

V. Development of Tissue Specificity

VI. Other Responses of Developing Systems to Implantation of Adult Tissues

VII. Concluding Remarks; Perspectives


15. Effects of Radiations on Cells

I. Introduction

II. Action of Radiations at the Molecular Level

III. Biochemical Effects

IV. Cytochemical Effects

V. Biological Effects

VI. Variables in Radiation Effects

VII. Alterations of Radiation Effects by Foreign Agents


Author Index

Subject Index


No. of pages:
© Academic Press 1959
Academic Press
eBook ISBN:

About the Editor

Jean Brachet

Alfred E. Mirsky

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