Techniques in Protein Chemistry

Techniques in Protein Chemistry

First published on August 28, 1980

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  • Editor: Choh Hao Li
  • eBook ISBN: 9780323162401

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Hormonal Proteins and Peptides: Techniques in Protein Chemistry, Volume IX presents the various methods in protein chemistry and discusses their use for hormonal protein investigations. This book examines the approach to investigating biologically active peptides, which is based on a combination of chromatographic and fluorometric procedures. Organized into five chapters, this volume starts with an overview of the fluorometric methods for analysis of peptides and proteins with focus on their use in high performance liquid chromatography. This text then presents a thorough discussion on partition chromatography of proteins and peptides. Other chapters consider a comprehensive account of absorption and fluorescence techniques for the structural analysis of proteins. This book presents as well a comprehensive discussion on optical rotatory dispersion and circular dichroism methods and their application for the study of protein conformation. The final chapter deals with the historical aspects of fetuin and ultracentrifuge. This book is a valuable resource for chemists, biologists, endocrinologists, and physicians.

Table of Contents

  • List of Contributors


    Contents of Previous Volumes

    1 Fluorometric Methods for Analysis of Proteins and Peptides: Principles and Applications

    I. Introduction

    II. Fluorogenic Reagents

    III. High Performance Liquid Chromatography (HPLC) of Proteins and Peptides

    IV. Detection Procedures for Proteins and Peptides

    V. Analysis of Prelabeled Proteins and Peptides

    VI. Amino Acid Analysis

    VII. Application to the Study of Opioid Peptides


    2 Partition and Partition Chromatography of Peptides and Proteins

    I. Introduction

    II. Theoretical Aspects of Partition Chromatography

    III. Techniques of Operation

    IV. Methodology and Applications

    V. Concluding Remarks


    3 The Structural Analysis of Polypeptide and Protein Hormones by Absorption and Fluorescence Spectroscopy

    I. Elements of Polypeptide Structure

    II. Absorption

    III. Fluorescence

    IV. Applications


    4 Measurement of Chiroptical Phenomena in Proteins

    I. Introduction

    II. Definitions, Symbols, and Units

    III. Experimental Measurement

    IV. Preparation of Samples

    V. Interpretation and Selected Examples

    VI. Concluding Remarks


    5 The Ultracentrifuge and Fetuin: Historical Aspects

    I. Introduction

    II. The First High-Speed Oil-Turbine Ultracentrifuge

    III. Further Development of the Ultracentrifuge

    IV. Fetuin

    V. α-Fetoproteins (AFP)

    VI. In Retrospect



Product details

  • No. of pages: 284
  • Language: English
  • Copyright: © Academic Press 1980
  • Published: August 28, 1980
  • Imprint: Academic Press
  • eBook ISBN: 9780323162401

About the Editor

Choh Hao Li

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