Techniques in Protein Chemistry - 1st Edition - ISBN: 9780124472099, 9780323162401

Techniques in Protein Chemistry

1st Edition

Editors: Choh Hao Li
eBook ISBN: 9780323162401
Imprint: Academic Press
Published Date: 28th August 1980
Page Count: 284
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Hormonal Proteins and Peptides: Techniques in Protein Chemistry, Volume IX presents the various methods in protein chemistry and discusses their use for hormonal protein investigations. This book examines the approach to investigating biologically active peptides, which is based on a combination of chromatographic and fluorometric procedures. Organized into five chapters, this volume starts with an overview of the fluorometric methods for analysis of peptides and proteins with focus on their use in high performance liquid chromatography. This text then presents a thorough discussion on partition chromatography of proteins and peptides. Other chapters consider a comprehensive account of absorption and fluorescence techniques for the structural analysis of proteins. This book presents as well a comprehensive discussion on optical rotatory dispersion and circular dichroism methods and their application for the study of protein conformation. The final chapter deals with the historical aspects of fetuin and ultracentrifuge. This book is a valuable resource for chemists, biologists, endocrinologists, and physicians.

Table of Contents

List of Contributors Preface Contents of Previous Volumes 1 Fluorometric Methods for Analysis of Proteins and Peptides: Principles and Applications I. Introduction II. Fluorogenic Reagents III. High Performance Liquid Chromatography (HPLC) of Proteins and Peptides IV. Detection Procedures for Proteins and Peptides V. Analysis of Prelabeled Proteins and Peptides VI. Amino Acid Analysis VII. Application to the Study of Opioid Peptides References 2 Partition and Partition Chromatography of Peptides and Proteins I. Introduction II. Theoretical Aspects of Partition Chromatography III. Techniques of Operation IV. Methodology and Applications V. Concluding Remarks References 3 The Structural Analysis of Polypeptide and Protein Hormones by Absorption and Fluorescence Spectroscopy I. Elements of Polypeptide Structure II. Absorption III. Fluorescence IV. Applications References 4 Measurement of Chiroptical Phenomena in Proteins I. Introduction II. Definitions, Symbols, and Units III. Experimental Measurement IV. Preparation of Samples V. Interpretation and Selected Examples VI. Concluding Remarks References 5 The Ultracentrifuge and Fetuin: Historical Aspects I. Introduction II. The First High-Speed Oil-Turbine Ultracentrifuge III. Further Development of the Ultracentrifuge IV. Fetuin V. α-Fetoproteins (AFP) VI. In Retrospect References Index


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© Academic Press 1980
Academic Press
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Choh Hao Li

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