Techniques in Protein Chemistry

Techniques in Protein Chemistry

Published Under the Auspices of the Protein Society

1st Edition - January 1, 1994

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  • Editor: John W. Crabb
  • eBook ISBN: 9781483217598

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Techniques in Protein Chemistry V highlights current methods in peptide and protein mass spectrometry, sequence and amino acid analysis, fragmentations, separations, protein folding and modeling, peptide and protein NMR, and peptide synthesis. This volume emerged from the manuscripts presented at the Seventh Symposium of the Protein Society, held in San Diego on July 24-28, 1993. This volume is organized into eight parts encompassing 61 chapters. The first part surveys the peptide and protein characterization, detection, and analysis by mass spectrometry. The subsequent parts describe the structural characterization and analysis of posttranslational processing events, as well as the characterization of protein and amino acid sequences using several analytical techniques. Other parts explore other analytical methods for peptide and protein separations; some aspects involved in protein design and functional domain analysis; and the evaluation of protein conformation, folding, and modeling. The last parts contain research papers on NMR analysis of peptide and protein solution structures. These parts also look into topics related to peptide synthesis and peptide libraries. This book is intended primarily for protein and analytical chemists.

Table of Contents

  • Foreword



    Section I Mass Spectrometry of Peptides and Proteins

    Identification of 2-D Gel Proteins at the Femtomole Level by Molecular Mass Searching of Peptide Fragments in a Protein Sequence Database

    Characterization by Mass Spectrometry of Conjugates Between Proteins and Low Molecular Weight Chemicals

    Protein Ladder Sequencing: Towards Automation

    Mass Spectrometric Protocol for the Analysis of UV-Crosslinked Protein- Nucleic Acid Complexes

    β-Lactamases: Probing the Mechanism of Action by Electrospray Ionisation Mass Spectrometry (ESI MS)

    Electrospray Mass Spectral Analysis of the (AT)-Arylazido-β-alanyl NAD+ Modified Bovine Heart Mitochondrial NADH Dehydrogenase

    Protease Digestions on PVDF Membranes for Matrix-Assisted Laser Desorption Mass Spectrometry

    Section II Analysis of Posttranslational Processing Events

    Structural Characterization of a Novel Tetrasaccharide Attached to Ser-61 of Human Factor IX by Mass Spectrometry and Ή NMR Spectroscopy

    Primary Structure of Gallinacin-1, an Antimicrobial β-Defensin from Chicken Leukocytes

    Site-Specific Glycosylation of Recombinant Stem Cell Factor

    Strategies for the Characterizaton of Carbohydrates from Glycoproteins by Mass Spectrometry

    Distinction between Phosphorylated and Sulfated Peptides by Matrix Assisted Laser Desorption Ionization Reflector Mass Spectrometry at the Sub Picomole Level

    Characterization of the Natural and Radiation Induced Modification of Histones

    Selective Detection of Thr-, Ser-, and Tyr-Phosphopeptides in Complex Digests by Electrospray LC-MS

    Section III Protein Sequencing and Amino Acid Analysis

    A Synthetic Peptide for Evaluating Protein Sequencing Capabilities: Design of ABRF-93SEQ and Results

    Combined Edman-Chemical and Laser-Desorption Mass Spectrometric Approaches to Micro Peptide Sequencing: Optimization and Applications

    Selective Sequencing of Peptides with N-Terminal Ser or Thr in Mixtures

    Characterization of Phosphorylation Sites in Bovine Rhodopsin Using Modified Gas Phase Sequencing Programs

    Strategies for Increasing the Sensitivity of N-Terminal Sequence Analysis

    Alkylation of Cysteine Residues with 3-Bromopropylamine: Identification by Protein Sequencing and Quantitation by Amino Acid Analysis

    Instability of Cysteine Labeling with 4-(Aminosulfonyl)-7-fluoro-2,l,3- benzoxadiazole (ABD-F)

    Elution of Proteins from Polyacrylamide Gels onto the Hewlett-Packard Sequencing Column

    High Sensitivity Peptide Sequence Analysis Using in Situ Proteolysis on High Retention PVDF Membranes and a Biphasic Reaction Column Sequencer

    A One-Step Enzymatic Digestion Procedure for PVDF-Bound Proteins That Does Not Require PVP-40

    Tryptic in Situ Digestion in the Presence of Dimethylsulfoxide

    Amino Acid Analysis of Phospho-Peptides: ABRF-93AAA

    Section IV Peptide and Protein Separations and Other Methods

    Protein and Peptide Recovery from Polyacrylamide Gels

    High-Resolution Large-Scale Isoelectric Focusing of Proteins in a Chemically Defined Buffer System

    Applications of Tandem Capillary HPLC in the Isolation of Proteins for Characterization Using Microsequencing and Mass Spectrometry

    Analytical Ultracentrifugation Analysis of the Self-Association of κ-Bungarotoxin

    A Facile Method for the Release, Labeling, and CE Analysis of Glycoprotein Oligosaccharides

    The Resonant Mirror: A Versatile Tool for the Study of Biomolecular Interactions

    Post-run Analysis of Proteins Purified by Capillary Electrophoresis with Membrane Fraction Collection

    Section V Protein Design and Functional Domain Analysis

    In Vitro Expression of Serpins

    Altering Substrate Specificity and Detecting Processivity in Nucleases

    Mutagenesis of n2-Site Metal-Binding Glutamate Residues in E. coli Glutamine Synthetase

    Reconstitution in Vitro of the Interleukin-6/Interleukin-6 Receptor Interaction: Direct Monitoring Using a Biosensor Employing Surface Plasmon Resonance Detection

    Functional Zone Identification by Biophysics Techniques: The Interaction of Calmodulin with Regulatory Peptides

    Characterization of the Reactive Cysteine Residues of the β-Galactosidase α-Acceptor Deletion Mutant EA22

    Characterization of a 32-Residue Peptide from Rat DNA Polymerase β with Single-Stranded DNA-Binding Affinity

    Mapping the Retinoid-Binding Domain of Cellular Retinaldehyde-Binding Protein

    Section VI Protein Conformation, Folding, and Modeling

    Probing the Structure of a Human Interleukin-6 Mutant by Limited Proteolysis

    Extraction and Characterization of Molecular Surface Knobs and Holes for the Study of Complex Formation

    Design of an Auto-Associative Neural Network with Hidden Layer Activations That Were Used to Reclassify Local Protein Structures

    The Effects of Local Environments on the Pattern of Amino-Acid Substitution in Homologous Protein Structures: The Role of Side-Chain to Main-Chain van der Waals Interactions

    Modeling Human Chorionic Gonadotropin Using Distance Geometry and Immunological Constraints

    Section VII NMR Analysis of Peptide and Protein Solution Structures

    NMR Methods Used to Derive Solution Conformations in Peptides

    Measuring Global and Local Structural Free Energy Changes in Staphylococcal Nuclease by NMR-Observed Hydrogen Exchange

    Mapping of Specific Protein-Protein Interactions by NMR

    Protein Folding Intermediates Characterized by Pulsed Hydrogen Exchange

    Application of Equilibrium and Stopped-Flow 19F NMR Spectroscopy to Protein Folding: Studies of E. coli Dihydrofolate Reductase

    Protein Hyperfine Resonance Assignments in Glycera dibranchiata Monomer Hemoglobin Component IV

    Section VIII Peptide Synthesis and Peptide Libraries

    FMOC Solid Phase Synthesis of PhosphopeptidesSynthesis of Uniform Peptide Libraries and Methods for Physico-Chemical Analysis

    A General Strategy for the Synthesis of Eosin Fluorescein Energy Transfer Substrates for High Sensitivity Screening of Protease Inhibitors

    Variable Success of Peptide-Resin Cleavage and Deprotection Following Solid- Phase Synthesis

    Membrane Protecting Sequences and New Antimicrobial Peptides Identified through the Screening of Synthetic Peptide Combinatorial Libraries

    Phage Display of Random Peptides on a Protein Scaffold

    New Methodologies in the Preparation of Peptide Libraries

    Using Peptoid Libraries [Oligo N-Substituted Glycines] for Drug Discovery

    Construction and Screening of Libraries of Peptide and Non-Peptide Structures


Product details

  • No. of pages: 588
  • Language: English
  • Copyright: © Academic Press 1994
  • Published: January 1, 1994
  • Imprint: Academic Press
  • eBook ISBN: 9781483217598

About the Editor

John W. Crabb

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