
Techniques in Clinical Chemistry
A Handbook for Medical Laboratory Technicians
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Techniques in Clinical Chemistry: A Handbook for Medical Laboratory Technicians is a five-chapter supplementary handbook on the fundamentals of medical laboratory test and protocols. The opening chapter covers the fundamental aspects of medical laboratories, including accuracy measures, methods of analysis, buffers, indicators, and valency. These topics are followed by discussions on the procedures for preliminary preparation, primarily for sample preparation and specimen collection. Considerable chapters are devoted to the scrutinized analysis of specific specimen, such as blood, cerebrospinal fluid, feces, gastric sample, and urine. The final chapter discusses technical essentials of renal and hepatic function tests. This handbook is directed toward medical and laboratory technicians and clinicians.
Table of Contents
I.—Fundamentals
Accuracy and Apparatus
Volumetric Analysis
Standard Solutions
Types of Volumetric Analysis
Iodometric Standardization of Solutions
To Standardize N/10 Hydrochloric Acid
Hydrogen Ion Concentration (pH)
Buffers
Indicators
Valency
II.—Preliminary Preparation
Cleaning of Glassware
Detergents
Collection of Pathological Specimens
Blood
Urine
Feces
Cerebrospinal Fluid
Gastric Contents
Exudates and Transudates
Preparation of Protein-Free Filtrates
III.—Examination of Blood
Blood-Urea
Non-Protein Nitrogen
Blood Uric Acid
Blood-Glucose
Blood-Cholesterol
Serum-Calcium
Plasma-Chlorides
Plasma-Proteins
Plasma-Sodium
Serum-Magnesium
Blood-Iron
Serum Inorganic Phosphorus
Serum-Potassium
Serum-Bromides
Serum-Phosphatase
Serum Ascorbic Acid
Serum-Carbon-Dioxide-Combining Power
Detection and Estimation of Serum-Bilirubin
IV.—Cerebrospinal Fluid Analysis
Estimation of
Chlorides
Urea and Glucose
Total Protein
'Globulin' Reactions
V.—Analysis of the Feces
Estimation of Fat
Detection of Occult Blood
Bilirubin
Urobilin
VI.—Gastric Analysis
Estimation of Chlorides
Detection of Organic Acids
Estimation of Free and Combined Hydrochloric Acid
The Fractional Test-Meal
VII.—Examination of Urine
Detection and Estimation of Glucose
The Glucose-Tolerance Test
Detection of Ketone Bodies
Estimation of Urine Chlorides
Estimation of Urine Urea
Estimation of Urine Diastase
Estimation of Urine Ascorbic Acid
Proteinuria
Indicanuria
Bilirubin and Urobilin
Blood—Hematuria and Hemoglobinuria
Melanogenuria
Alkaptonuria
Porphyrinuria
VIII.—Tests of Renal and Hepatic Function
Tests for Renal Efficiency:
Urea Concentration Test
Urea Clearance Test
Urine Concentration Test
Urine Dilution Test
Liver Function Tests:
Thymol Turbidity Test
Thymol Flocculation Test
Appendix
1.—Normal Blood Values
2.—Atomic Weights
3.—Calibration of Glassware with Mercury
4.—Dichromate Cleaning Fluid
5.—Preparation of Stop-Cock Grease
6.—Normality of Concentrated Acids and Bases
7.—Accuracy of Volumetric Flasks
8.—Specifications for Graduated Pipettes
9.—Specifications for One-Mark Bulb Pipettes
10.—Specifications for Burettes
Index
Product details
- No. of pages: 182
- Language: English
- Copyright: © Butterworth-Heinemann 1954
- Published: January 1, 1954
- Imprint: Butterworth-Heinemann
- eBook ISBN: 9781483221267
About the Author
Frederick N. Bullock
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