Techniques in Clinical Chemistry

Techniques in Clinical Chemistry

A Handbook for Medical Laboratory Technicians

1st Edition - January 1, 1954

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  • Author: Frederick N. Bullock
  • eBook ISBN: 9781483221267

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Description

Techniques in Clinical Chemistry: A Handbook for Medical Laboratory Technicians is a five-chapter supplementary handbook on the fundamentals of medical laboratory test and protocols. The opening chapter covers the fundamental aspects of medical laboratories, including accuracy measures, methods of analysis, buffers, indicators, and valency. These topics are followed by discussions on the procedures for preliminary preparation, primarily for sample preparation and specimen collection. Considerable chapters are devoted to the scrutinized analysis of specific specimen, such as blood, cerebrospinal fluid, feces, gastric sample, and urine. The final chapter discusses technical essentials of renal and hepatic function tests. This handbook is directed toward medical and laboratory technicians and clinicians.

Table of Contents


  • I.—Fundamentals

    Accuracy and Apparatus

    Volumetric Analysis

    Standard Solutions

    Types of Volumetric Analysis

    Iodometric Standardization of Solutions

    To Standardize N/10 Hydrochloric Acid

    Hydrogen Ion Concentration (pH)

    Buffers

    Indicators

    Valency

    II.—Preliminary Preparation

    Cleaning of Glassware

    Detergents

    Collection of Pathological Specimens

    Blood

    Urine

    Feces

    Cerebrospinal Fluid

    Gastric Contents

    Exudates and Transudates

    Preparation of Protein-Free Filtrates

    III.—Examination of Blood

    Blood-Urea

    Non-Protein Nitrogen

    Blood Uric Acid

    Blood-Glucose

    Blood-Cholesterol

    Serum-Calcium

    Plasma-Chlorides

    Plasma-Proteins

    Plasma-Sodium

    Serum-Magnesium

    Blood-Iron

    Serum Inorganic Phosphorus

    Serum-Potassium

    Serum-Bromides

    Serum-Phosphatase

    Serum Ascorbic Acid

    Serum-Carbon-Dioxide-Combining Power

    Detection and Estimation of Serum-Bilirubin

    IV.—Cerebrospinal Fluid Analysis

    Estimation of

    Chlorides

    Urea and Glucose

    Total Protein

    'Globulin' Reactions

    V.—Analysis of the Feces

    Estimation of Fat

    Detection of Occult Blood

    Bilirubin

    Urobilin

    VI.—Gastric Analysis

    Estimation of Chlorides

    Detection of Organic Acids

    Estimation of Free and Combined Hydrochloric Acid

    The Fractional Test-Meal

    VII.—Examination of Urine

    Detection and Estimation of Glucose

    The Glucose-Tolerance Test

    Detection of Ketone Bodies

    Estimation of Urine Chlorides

    Estimation of Urine Urea

    Estimation of Urine Diastase

    Estimation of Urine Ascorbic Acid

    Proteinuria

    Indicanuria

    Bilirubin and Urobilin

    Blood—Hematuria and Hemoglobinuria

    Melanogenuria

    Alkaptonuria

    Porphyrinuria

    VIII.—Tests of Renal and Hepatic Function

    Tests for Renal Efficiency:

    Urea Concentration Test

    Urea Clearance Test

    Urine Concentration Test

    Urine Dilution Test

    Liver Function Tests:

    Thymol Turbidity Test

    Thymol Flocculation Test

    Appendix

    1.—Normal Blood Values

    2.—Atomic Weights

    3.—Calibration of Glassware with Mercury

    4.—Dichromate Cleaning Fluid

    5.—Preparation of Stop-Cock Grease

    6.—Normality of Concentrated Acids and Bases

    7.—Accuracy of Volumetric Flasks

    8.—Specifications for Graduated Pipettes

    9.—Specifications for One-Mark Bulb Pipettes

    10.—Specifications for Burettes

    Index


Product details

  • No. of pages: 182
  • Language: English
  • Copyright: © Butterworth-Heinemann 1954
  • Published: January 1, 1954
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483221267

About the Author

Frederick N. Bullock

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