Techniques in Clinical Chemistry

Techniques in Clinical Chemistry: A Handbook for Medical Laboratory Technicians is a five-chapter supplementary handbook on the fundamentals of medical laboratory test and protocols.
The opening chapter covers the fundamental aspects of medical laboratories, including accuracy measures, methods of analysis, buffers, indicators, and valency. These topics are followed by discussions on the procedures for preliminary preparation, primarily for sample preparation and specimen collection. Considerable chapters are devoted to the scrutinized analysis of specific specimen, such as blood, cerebrospinal fluid, feces, gastric sample, and urine. The final chapter discusses technical essentials of renal and hepatic function tests. This handbook is directed toward medical and laboratory technicians and clinicians.

I.—Fundamentals

Accuracy and Apparatus

Volumetric Analysis

Standard Solutions

Types of Volumetric Analysis

Iodometric Standardization of Solutions

To Standardize N/10 Hydrochloric Acid

Hydrogen Ion Concentration (pH)

Buffers

Indicators

Valency

II.—Preliminary Preparation

Cleaning of Glassware

Detergents

Collection of Pathological Specimens

Blood

Urine

Feces

Cerebrospinal Fluid

Gastric Contents

Exudates and Transudates

Preparation of Protein-Free Filtrates

III.—Examination of Blood

Blood-Urea

Non-Protein Nitrogen

Blood Uric Acid

Blood-Glucose

Blood-Cholesterol

Serum-Calcium

Plasma-Chlorides

Plasma-Proteins

Plasma-Sodium

Serum-Magnesium

Blood-Iron

Serum Inorganic Phosphorus

Serum-Potassium

Serum-Bromides

Serum-Phosphatase

Serum Ascorbic Acid

Serum-Carbon-Dioxide-Combining Power

Detection and Estimation of Serum-Bilirubin

IV.—Cerebrospinal Fluid Analysis

Estimation of

Chlorides

Urea and Glucose

Total Protein

'Globulin' Reactions

V.—Analysis of the Feces

Estimation of Fat

Detection of Occult Blood

Bilirubin

Urobilin

VI.—Gastric Analysis

Estimation of Chlorides

Detection of Organic Acids

Estimation of Free and Combined Hydrochloric Acid

The Fractional Test-Meal

VII.—Examination of Urine

Detection and Estimation of Glucose

The Glucose-Tolerance Test

Detection of Ketone Bodies

Estimation of Urine Chlorides

Estimation of Urine Urea

Estimation of Urine Diastase

Estimation of Urine Ascorbic Acid

Proteinuria

Indicanuria

Bilirubin and Urobilin

Blood—Hematuria and Hemoglobinuria

Melanogenuria

Alkaptonuria

Porphyrinuria

VIII.—Tests of Renal and Hepatic Function

Tests for Renal Efficiency:

Urea Concentration Test

Urea Clearance Test

Urine Concentration Test

Urine Dilution Test

Liver Function Tests:

Thymol Turbidity Test

Thymol Flocculation Test

Appendix

1.—Normal Blood Values

2.—Atomic Weights

3.—Calibration of Glassware with Mercury

4.—Dichromate Cleaning Fluid

5.—Preparation of Stop-Cock Grease

6.—Normality of Concentrated Acids and Bases

7.—Accuracy of Volumetric Flasks

8.—Specifications for Graduated Pipettes

9.—Specifications for One-Mark Bulb Pipettes

10.—Specifications for Burettes

Index