Secure CheckoutPersonal information is secured with SSL technology.
Free ShippingFree global shipping
No minimum order.
Chapter 1. High-resolution temporal analysis of global promoter activity in Bacillus subtilis
2. Gene Fusion Technology
3. Reporter proteins
4. Cellular Placement of Transcriptional Fusions
5. Choice of System
6. Use of Promoter Fusions to Study Biological Processes at High Temporal Resolution in Bacteria
7. Methodology for High-throughput Analysis of Promoter Activity with Fine Temporal Resolution in B. subtilis
Chapter 2. Data mining for microbiologists
2. What is Data Mining?
3. The Data Mining Process
4. Inferential Techniques
5. Machine Learning Techniques
6. The Role of eScience
7. Case Study: Data Mining for Protein Function Prediction
8. Data Mining with Microbial Data: Practical Issues
Chapter 3. Proteomics
2. Absolute Quantification Workflows in Proteomics
3. The Proteomics Workflow
4. Generation of Absolute Quantitative Data by Targeted Mass Spectrometry
5. Generation of Large-Scale Relative Proteomics Data: Differential 2D Gel Image Analysis
6. Large-Scale Absolute Quantitative Proteomics with SRM-Calibrated 2D PAGE
Chapter 4. Imaging fluorescent protein fusions in live bacteria
2. Molecular Toolkits
3. Functional Analysis
4. Growth Conditions
5. Agarose Slide Preparation
6. Imaging Hardware
7. Imaging Software
8. Image Processing
9. Concluding Remarks
Chapter 5. Targeted and quantitative metabolomics in bacteria
2. Cultivation of Bacteria for Metabolome Analysis
3. Sampling Bacterial Cultures for Metabolome Analysis
4. Extraction of Metabolites from Bacteria
5. Analysis by Mass Spectrometry
6. Interpretation of Metabolite Data
Chapter 6. Array-based approaches to bacterial transcriptome analysis
2. Prior Considerations
3. Performing the Experiments
4. Data Analysis
5. Final Comments
Focusing on the systems biology of bacteria and microorganisms, the 39th volume of Methods in Microbiology investigates the interface between molecular biology, bioinformatics, and modelling and predicting behavior. This cutting-edge research area is of extreme importance to the field and is developing quickly.
- Written by research experts in the field
- Provides a guide for lab scientists, researchers, and students
- Focuses on up-to-date research methods
Microbiologists, lab researchers, students
- No. of pages:
- © Academic Press 2012
- 28th November 2012
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
University of Newcastle upon Tyne
Colin Harwwod is a professor at Newcastle University
Newcastle University, UK
Elsevier.com visitor survey
We are always looking for ways to improve customer experience on Elsevier.com.
We would like to ask you for a moment of your time to fill in a short questionnaire, at the end of your visit.
If you decide to participate, a new browser tab will open so you can complete the survey after you have completed your visit to this website.
Thanks in advance for your time.