Description

Focusing on the systems biology of bacteria and microorganisms, the 39th volume of Methods in Microbiology investigates the interface between molecular biology, bioinformatics, and modelling and predicting behavior. This cutting-edge research area is of extreme importance to the field and is developing quickly. 

Key Features

  • Written by research experts in the field
  • Provides a guide for lab scientists, researchers, and students
  • Focuses on up-to-date research methods

Readership

Microbiologists, lab researchers, students

 

Table of Contents

Series Page

Series Advisors

Contributors

Chapter 1. High-resolution temporal analysis of global promoter activity in Bacillus subtilis

1. Introduction

2. Gene Fusion Technology

3. Reporter proteins

4. Cellular Placement of Transcriptional Fusions

5. Choice of System

6. Use of Promoter Fusions to Study Biological Processes at High Temporal Resolution in Bacteria

7. Methodology for High-throughput Analysis of Promoter Activity with Fine Temporal Resolution in B. subtilis

8. Conclusions

References

Chapter 2. Data mining for microbiologists

1. Introduction

2. What is Data Mining?

3. The Data Mining Process

4. Inferential Techniques

5. Machine Learning Techniques

6. The Role of eScience

7. Case Study: Data Mining for Protein Function Prediction

8. Data Mining with Microbial Data: Practical Issues

9. Conclusions

References

Chapter 3. Proteomics

Abbreviations

1. Introduction

2. Absolute Quantification Workflows in Proteomics

3. The Proteomics Workflow

4. Generation of Absolute Quantitative Data by Targeted Mass Spectrometry

5. Generation of Large-Scale Relative Proteomics Data: Differential 2D Gel Image Analysis

6. Large-Scale Absolute Quantitative Proteomics with SRM-Calibrated 2D PAGE

References

Chapter 4. Imaging fluorescent protein fusions in live bacteria

1. Introduction

2. Molecular Toolkits

3. Functional Analysis

4. Growth Conditions

5. Agarose Slide Preparation

6. Imaging Hardware

7. Imaging Software

8. Image Processing

9. Concluding Remarks

References

Chapter 5. Targeted and quantitative metabolomics in bacteria

1. Introduction

2. Cultivation of Bacteria for Metabolome Analysis

3. Sampling

Details

No. of pages:
200
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
eBook ISBN:
9780124072015
Print ISBN:
9780080993874

About the serial-volume-editors

Anil Wipat

Affiliations and Expertise

University of Newcastle upon Tyne

Colin Harwood

Colin Harwwod is a professor at Newcastle University

Affiliations and Expertise

Newcastle University, UK