This newest edition to the Laboratory Techniques Series gives current state of the art use of synthetic peptides in molecular biology and practical protocols on how to conjugate peptides, immunize animals with peptides and monitor immune responses to peptides in vitro.
It gives background information on antigenic specificity, prediction of antigenic sites in proteins and applications of peptides in immunology and virology, as probes in diagnosis and as vaccines. The book also describes antigenicity of proteins and methods to localize antigenic sites as well as methods for predicting epitoxes, and gives detailed protocols for peptide-carrier conjugation, immunization with peptides, and peptide immunoassays.
The volume also describes typical use of antipeptide antibodies in molecular and cellular biology as well as the use of peptides in the diagnosis of viral infections and autoimmune diseases, and the use of peptides as potential synthetic vaccines. An excellent edition to an excellent series, available in hardbound and paperback.
Acknowledgments. List of Abbreviations.
- Molecular Dissection of Protein Antigens and the Prediction of Epitopes (M.H.V. Van Regenmortel). 1.1 Introduction. 1.2 Definition of antigenicity and the concept of epitope. 1.3 Types of epitopes. 1.4 Methods used for localizing epitopes. 1.5 The antigenic structure of model proteins. 1.6 Antigenicity prediction. 1.7 T cell epitopes.
2. Peptide-Carrier Conjugation (S. Muller).
2.2 Choice of carrier.
2.3 Optimal peptide density on carrier protein.
2.4 Point of attachment on peptide chain.
2.5 Chemical coupling.
2.6 Photochemical coupling.
2.7 Coupling of peptides to liposomes.
2.8 Coupling of peptides to solid supports.
2.9 Determination of peptide: carrier ratio of conjugates.
2.10 Peptide derivatization.
3. Immunization with Peptides (S. Muller). 3.1 Introduction. 3.2 The choice of animal. 3.3 The immunogen. 3.4 The adjuvant. 3.5 The route of injection. 3.6 Specific immunization protocols. 3.7 Concluding remarks.
4. Peptide Immunoassays (M.V.H. Van Regenmortel). 4.1 Introduction. 4.2 Types of solid-phase immunoassays. 4.3 Solid-phase immunoassay procedures. 4.4 Dot immunobinding assay. 4.5 Spotscan assay. 4.6 Biosensor assays. 4.7 Measurement of affinity constants. 4.8 Monitoring of the immune response to peptides.
5. Use of Antipeptide Antibodies in Molecular and Cellular Biology (S. Muller). 5.1 Detect
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- © Elsevier Science 1999
- 25th November 1999
- Elsevier Science
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@from:(P. Meyer) @qu:Immunoassay of peptides, their use for different types of diagnosis, and the evaluation of synthetic peptides as vaccines all point to the interest of this book both at a fundamental level as well as in terms of applications. (...)useful illustrations can be found in its over 300 pages. @source:Res. Microbiology Vol. 151 @from:(C.-J. van Oss) @qu:This work is strongly recommended to all clinical, diagnostic and research Immunologists and Immunochemists. @source:Immunological Invest., Vol. 29, No. 4