Structural and Resonance Techniques in Biological Research

Structural and Resonance Techniques in Biological Research

1st Edition - January 28, 1984

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  • Editor: Denis Rousseau
  • eBook ISBN: 9780323148306

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Structural and Resonance Techniques in Biological Research discusses the fundamentals and applications of nuclear magnetic resonance, electron spin resonance, Mössbauer spectroscopy, x-ray absorption spectroscopy, macromolecular crystallography, and small-angle x-ray scattering and diffraction. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Optical Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.

Table of Contents

  • List of Contributors

    Series Introduction


    1. Nuclear Magnetic Resonance

    I. Introduction

    II. Introduction to Nuclear Magnetic Resonance

    III. Theory

    IV. Experimental Considerations

    V. Applications

    VI. Concluding Remarks



    2. Electron Spin Resonance

    I. Introduction

    II. Principles of Electron Spin Resonance

    III. Establishing and Detecting Electron Spin Resonance

    IV. Applications of Direct (Continuous-Wave) ESR

    V. Resonance as a Perturbation

    VI. An ESR Perspective

    Appendix A. ESR Hardware, Glassware, and Supplies

    Appendix B. ESR Software

    Appendix C. Calibration of ESR Spectra



    3. Mössbauer Spectroscopy

    I. Introduction

    II. Principles of Mössbauer Spectroscopy

    III. Experimental Techniques

    IV. Applications to Isolated Biomolecules

    V. Applications to Molecular Systems

    VI. Applications to Tissue

    VII. Applications to Whole or Part Organisms: Uptake and Metabolism

    VIII. Measurement of Vibration and Movement

    IX. Conclusions


    4. X-Ray Absorption Spectroscopy

    I. Introduction

    II. Theory

    III. Experimental Considerations

    IV. EXAFS as a Structural Technique in Biology

    V. Conclusion


    5. Macromolecular Crystallography

    I. Introduction

    II. Crystallization of Macromolecules

    III. X-Ray Diffraction from Crystals

    IV. Phase Determination

    V. Heavy-Atom Derivatives

    VI. The Electron-Density Map

    VII. New Directions

    Appendix A. The Basic Mathematics of Crystallography

    Appendix B. A Specific Strategy for Protein Crystallization

    Appendix C. A Crystallographic Paper Primer

    Appendix D. Suggestions for Further Reading


    6. Small-Angle X-Ray Scattering and Diffraction

    I. Introduction

    II. X-Ray Scattering and Diffraction

    III. X-Ray Technology

    IV. Biological Applications

    V. Summary



Product details

  • No. of pages: 490
  • Language: English
  • Copyright: © Academic Press 1984
  • Published: January 28, 1984
  • Imprint: Academic Press
  • eBook ISBN: 9780323148306

About the Editor

Denis Rousseau

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