Structural and Resonance Techniques in Biological Research - 1st Edition - ISBN: 9780125993203, 9780323148306

Structural and Resonance Techniques in Biological Research

1st Edition

Editors: Denis Rousseau
eBook ISBN: 9780323148306
Imprint: Academic Press
Published Date: 28th January 1984
Page Count: 490
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Structural and Resonance Techniques in Biological Research discusses the fundamentals and applications of nuclear magnetic resonance, electron spin resonance, Mössbauer spectroscopy, x-ray absorption spectroscopy, macromolecular crystallography, and small-angle x-ray scattering and diffraction. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Optical Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.

Table of Contents

List of Contributors

Series Introduction


1. Nuclear Magnetic Resonance

I. Introduction

II. Introduction to Nuclear Magnetic Resonance

III. Theory

IV. Experimental Considerations

V. Applications

VI. Concluding Remarks



2. Electron Spin Resonance

I. Introduction

II. Principles of Electron Spin Resonance

III. Establishing and Detecting Electron Spin Resonance

IV. Applications of Direct (Continuous-Wave) ESR

V. Resonance as a Perturbation

VI. An ESR Perspective

Appendix A. ESR Hardware, Glassware, and Supplies

Appendix B. ESR Software

Appendix C. Calibration of ESR Spectra



3. Mössbauer Spectroscopy

I. Introduction

II. Principles of Mössbauer Spectroscopy

III. Experimental Techniques

IV. Applications to Isolated Biomolecules

V. Applications to Molecular Systems

VI. Applications to Tissue

VII. Applications to Whole or Part Organisms: Uptake and Metabolism

VIII. Measurement of Vibration and Movement

IX. Conclusions


4. X-Ray Absorption Spectroscopy

I. Introduction

II. Theory

III. Experimental Considerations

IV. EXAFS as a Structural Technique in Biology

V. Conclusion


5. Macromolecular Crystallography

I. Introduction

II. Crystallization of Macromolecules

III. X-Ray Diffraction from Crystals

IV. Phase Determination

V. Heavy-Atom Derivatives

VI. The Electron-Density Map

VII. New Directions

Appendix A. The Basic Mathematics of Crystallography

Appendix B. A Specific Strategy for Protein Crystallization

Appendix C. A Crystallographic Paper Primer

Appendix D. Suggestions for Further Reading


6. Small-Angle X-Ray Scattering and Diffraction

I. Introduction

II. X-Ray Scattering and Diffraction

III. X-Ray Technology

IV. Biological Applications

V. Summary




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© Academic Press 1984
Academic Press
eBook ISBN:

About the Editor

Denis Rousseau

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