Stem Cell Culture, Volume 86

1st Edition

Editors: Jennie Mather
Hardcover ISBN: 9780123738769
eBook ISBN: 9780080878041
Imprint: Academic Press
Published Date: 15th May 2008
Page Count: 416
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Table of Contents

Contributors to Volume 86

Preface

Biography

Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions

I Introduction

II Methods

III Discussion

IV Materials

Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder‐Free Conditions

I Introduction

II Methods and Materials

III Results

IV Discussion and Summary

Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation

I Introduction

II Rationale

III Methods

IV Summary

Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal

I Introduction

II Rationale

III Methods

IV Materials

V Discussion

Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability

I Introduction

II Amniotic Fluid Cell Composition and Stem Cells

III In Vitro Potential of Amniotic Fluid Stem Cells

IV Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells

V Discussion

Method to Isolate Mesenchymal‐Like Cells from Wharton's Jelly of Umbilical Cord

I Introduction

II Rationale

III Methods

IV Materials

V UCMSCs and Culture Characteristics

VI Discussion

Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)

I Introduction

II Methods

III Concluding Remarks

Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance

I Introduction

II Epithelial–Mesenchymal Relationship

III Stem Cells and Lineage Biology

IV Liver Processing

V Fractionation of Liver Cell Subpopulations

VI Feeder Cells

VII Extracellular Matrix

VIII Culture Me


Description

The purpose of this volume is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.

Key Features

  • Describes techniques in stem cell research
  • Delineates critical steps and potential pitfalls for each method *covers specific procedures in dealing with Human Embryonic Stem Cells

Readership

This book will fill the need for an introduction for biologist who are considering culturing stem cells in their lab.


Details

No. of pages:
416
Language:
English
Copyright:
© Academic Press 2008
Published:
Imprint:
Academic Press
eBook ISBN:
9780080878041
Hardcover ISBN:
9780123738769

About the Editors

Jennie Mather Editor

Affiliations and Expertise

Founder, President and CSO, Raven biotechnologies, Inc., South San Francisco, CA, USA