Stem Cell Culture

Stem Cell Culture

1st Edition - May 15, 2008
  • Editor: Jennie Mather
  • eBook ISBN: 9780080878041
  • Hardcover ISBN: 9780123738769

Purchase options

Purchase options
DRM-free (EPub, Mobi, PDF)
Available
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order

Description

The purpose of Stem Cell Culture is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.

Key Features

  • Describes techniques in stem cell research
  • Delineates critical steps and potential pitfalls for each method
  • Covers specific procedures in dealing with Human Embryonic Stem Cells

Readership

Biologists who are considering culturing stem cells in their lab.

Table of Contents

  • Contributors to Volume 86

    Preface

    Biography

    Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions

    I Introduction

    II Methods

    III Discussion

    IV Materials

    Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder‐Free Conditions

    I Introduction

    II Methods and Materials

    III Results

    IV Discussion and Summary

    Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation

    I Introduction

    II Rationale

    III Methods

    IV Summary

    Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal

    I Introduction

    II Rationale

    III Methods

    IV Materials

    V Discussion

    Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability

    I Introduction

    II Amniotic Fluid Cell Composition and Stem Cells

    III In Vitro Potential of Amniotic Fluid Stem Cells

    IV Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells

    V Discussion

    Method to Isolate Mesenchymal‐Like Cells from Wharton's Jelly of Umbilical Cord

    I Introduction

    II Rationale

    III Methods

    IV Materials

    V UCMSCs and Culture Characteristics

    VI Discussion

    Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)

    I Introduction

    II Methods

    III Concluding Remarks

    Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance

    I Introduction

    II Epithelial–Mesenchymal Relationship

    III Stem Cells and Lineage Biology

    IV Liver Processing

    V Fractionation of Liver Cell Subpopulations

    VI Feeder Cells

    VII Extracellular Matrix

    VIII Culture Media

    IX Monolayer Cultures of Cells

    X Three‐Dimensional Systems

    Acknowledgments

    Appendix 1: Nomenclature, Glossary, and Abbreviations

    Appendix 2: Details on the Preparation of Buffers/Reagents

    Appendix 3: Commercial Sources of Reagents

    Appendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells. (See Below for Suppliers and the Location of the Suppliers)

    Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo

    I Introduction

    II Rationale

    III Methods

    IV Materials

    V Discussion

    VI Summary

    Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells

    I Introduction

    II Rationale

    III Methods

    IV Materials

    V Discussion

    VI Summary

    Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue

    I Introduction

    II Isolation of hMSCs from Trabecular Bone

    III Isolation of hMSCs from Adipose Tissue

    IV Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs

    V Surface Marker Characterization of hMSCs

    VI hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways

    VII Summary

    Culture of Mesenchymal Stem/Progenitor Cells in Adhesion‐Independent Conditions

    I Introduction

    II Suspension Culture of Bone Marrow‐Derived MPCs

    III Analysis of Suspension Culture

    IV Mechanism of MPC Expansion in Suspension

    V Discussion

    Purification and Long‐Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes

    I Introduction

    II Materials and Methods: Cell Analysis and Purification by Flow Cytometry

    III Materials and Methods: Long‐Term Culture of Human Blood Vessel‐Associated Progenitor Cells

    IV Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes

    V Materials and Methods: Genotypic and Phenotypic Analyses of Long‐Term Cultured Cells

    VI Conclusion

    Isolation and Culture of Colon Cancer Stem Cells

    I Introduction

    II Identification of CSCs in Colon Cancer Tissue

    III Isolation and Propagation of Colon CSCs

    IV Discussion

    Isolation and Establishment of Human Tumor Stem Cells

    I Introduction

    II Rationale

    III Methods

    IV Results

    V Discussion

    VI Materials

    Stem Cells from Cartilaginous and Bony Fish

    I Introduction to Models and Uses

    II Embryonal Stem Cells

    III Tissue‐Specific Stem Cells

    IV Outlook and Future Contributions

    Index

    Volume in Series

Product details

  • No. of pages: 416
  • Language: English
  • Copyright: © Academic Press 2008
  • Published: May 15, 2008
  • Imprint: Academic Press
  • eBook ISBN: 9780080878041
  • Hardcover ISBN: 9780123738769

About the Editor

Jennie Mather

Affiliations and Expertise

Founder, President and CSO, Raven biotechnologies, Inc., South San Francisco, CA, USA