Description

The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of ‘best practice’ methods in the area of tissue engineering to help ensure the creation of safe, high quality products. Standardisation in cell and tissue engineering introduces concepts and current practice in the field of cell and tissue engineering to a wide audience and aims to provide awareness of the importance of standardisation in this area while suggesting directions for further investigation.

Part one provides an overview of methods for cell and tissue engineering and includes chapters on the fundamentals of cell and matrix biology for tissue engineering, 3D collagen biomatrix development, and control and vascularisation of tissue-engineered constructs. Part two begins with a chapter exploring the methods and protocols of standardisation in cell and tissue engineering before moving on to highlight issues of quality control in cell and tissue engineering, standardised chemical analysis and testing of biomaterials and principles of good laboratory practice (GLP) for in vitro cell culture applications.

Standardisation in cell and tissue engineering is a standard reference for leading research groups, government agencies, regulatory bodies, and researchers and technicians at all levels across the whole range of disciplines using cell culture within the pharmaceutical, biotechnology and biomedical industries.

Key Features

  • Introduces concepts and current practice in the field of cell and tissue engineering
  • Highlights the importance of standardisation in cell and tissue engineering and suggests directions for further investigation
  • Explores methods and protocols of standardisation in cell and tissue engineering and issues of quality control in cell and tissue engineering

Readership

Biomaterials scientists; Tissue and cell engineers

Table of Contents

Contributor contact details

Woodhead Publishing Series in Biomaterials

Foreword

Introduction

Part I: Methods for cell and tissue engineering

Chapter 1: Fundamentals of cell and matrix biology for tissue engineering

Abstract:

1.1 Introduction

1.2 Extracellular matrices (ECMs)

1.3 ECM and cell interaction

1.4 ECM and mechanical signalling

1.5 Future trends

1.6 Conclusion

Chapter 2: Three-dimensional collagen biomatrix development and control

Abstract:

2.1 Engineering cell-rich and matrix-rich tissues using collagen scaffolds

2.2 Controlling the mechanical properties of collagen

2.3 Architectural features: introducing elements of tissue complexity

2.4 Future trends

Chapter 3: Two- and three-dimensional tissue culture bioprocessing methods for soft tissue engineering

Abstract:

3.1 Introduction

3.2 Bioreactor configurations

3.3 Selecting scaffold materials and architectures for your bioreactor

3.4 Mass transfer in tissue engineering bioreactors

3.5 Important parameters and taking measurements of bioreactor cultures

3.6 Tissue engineering process design

3.7 Future trends

3.8 Conclusion

3.9 Sources of further information and advice

Chapter 4: Two- and three-dimensional tissue culture methods for hard tissue engineering

Abstract:

4.1 Introduction

4.2 Culture of bone and cartilage cells

4.3 Cell culture parameters: bone tissue culture

4.4 Cell culture parameters: cartilage tissue culture

4.5 Two-dimensional tissue culture methods for hard tissues

4.6 Two-and-a-half- and three-dimensional tissue culture methods for hard tissues

4.7 Conclusion

Chapter 5: Vascularisation of tissue-engineered constructs

Abstract:

5.1 Introduction

5.2 Growth of

Details

No. of pages:
276
Language:
English
Copyright:
© 2013
Published:
Imprint:
Woodhead Publishing
eBook ISBN:
9780857098726
Print ISBN:
9780857094193

About the editor

V Salih

Dr Vehid Salih is Reader at Peninsula Dental School, Plymouth University Schools of Medicine and Dentistry, UK.

Affiliations and Expertise

Plymouth University Schools of Medicine and Dentistry, UK

Reviews

"...a comprehensive overview of the current standards in cell and tissue engineering, and biomaterials, which is of interest to a broad public including biologists, biomedical engineers and chemists."--Biomat.net, December 2014

“A text such as this is needed to begin (at last) to crystalize some of the most common concepts and approaches and alert early entrants or students to the need for, and demands of standardisation…as a teaching primer and as a dip-in reference book, this is a really useful text.”--MaterialsView.com,July 1, 2014