Standard Methods of Clinical Chemistry - 1st Edition - ISBN: 9780126091069, 9781483282206

Standard Methods of Clinical Chemistry

1st Edition

By the American Association of Clinical Chemists

Editors: Roderick P. MacDonald
eBook ISBN: 9781483282206
Imprint: Academic Press
Published Date: 1st January 1970
Page Count: 298
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Standard Methods of Clinical Chemistry, Volume 6 provides information pertinent to the more accurate methods of analysis. This book deals with various subjects, including personnel management, electronics, and data processing systems.

Organized into 21 chapters, this volume begins with an overview of the most colorimetric methods for estimating uric acid based on the nonspecific reduction of phosphotungstate by uric acid in an alkaline solution. This text then examines the electrophoretic separation and quantitation of proteins in serum or other body fluids. Other chapters provide a discussion of the control of the major reaction variables needed to meet the recommendations of the International Union of Biochemistry. This book discusses as well the modifications developed to eliminate some of the inaccuracies resulting from incomplete destruction of hydrogen peroxide and instability of the developed chromophore. The final chapter deals with the enzymatic methods for the determination of lactic and pyruvic acids in body fluids and tissues.

This book is a valuable resource for clinical chemists.

Table of Contents

Harry H. Sobotka—A Biographical Sketch



Uric Acid (Uricase)

Cellulose Acetate Electrophoresis of Protiens of Serum, Cerebrospinal Fluid, and Urine

The International Unit: Standardization of Enzyme Activity Units

Hydroxyproline in Urine and Tissues

Porphyrins, Porphobilinogen, and Aminolevulinic Acid in Urine

Toxicology in a General Hospital

Spectrophotometric Quantitation of Plasma and Urinary Amino Nitrogen with Fluorodinitrobenzene

Colorimetric Determination of Vanilmandelic Acid in Urine

Hemoglobin in Plasma

Ion-Exchange Method for the Determination of Plasma Ammonia Nitrogen with the Berthelot Reaction

Electrophoretic Determination of Serum Lipoproteins (Prestaining Technique)

The Determination of Total Seromucoid (as Hexose)

Glatamic Oxalacetic Transaminase (Colorimetric)

The Determination of Glocuse by the Ortho-Toluidine Method (Filtrate and Direct Procedure)

Oxygen, Partial Pressure (PO2) Electrode Method

Iodometric Measurement of Amylase (Caraway)

Serum Lithium Determination by Atomic Absorption Spectroscopy


Calcium (Fluorometric)

Triglycerides ("Neutral Fat") in Serum

Nomenclature for Quantities and Units

Enzymatic Methods for Lactic and Pyruvic Acids

Author Index 261

Subject Index


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About the Editor

Roderick P. MacDonald

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