Spin Resonance Spectroscopy

Unit 1: Principle NMR and pulsed NMR
Introduction – Spin Magnetic properties – Resonance condition – Larmor Frequency  –  NMR instrumentation – comparison of NMR & EPR  –  chemical shift  –  spin-spin coupling – Coupling constant – first and second order spectra – simplification of complex spectra  – Boltzmann Statistics  –  Pulsed NMR  –  Basic principle of FT technique – Relaxation process – Bloch equations – Relaxation times – Line shape and line width analysis

Unit 2: 1H and 13C NMR spectroscopy
 Interpretation of 1H NMR – assignment of signals  – influence of factors on chemical shift of protons  – 13C NMR – spin decoupling – double resonance  –  Nuclear Overhauser Effect  –  Off Resonance Decoupling – CIDNP – 13C assignment of signals – additivity rule – calculation of chemical shifts for aromatic and aliphatic compounds – 2D NMR ––  DEPT 13C  – 13C correlation COSY, HETCOR, NOE & NOSEY- multidimensional NMR

Unit 3: Applications of NMR
Applications of  31P, 19F and 15N – NMR spectroscopy used in structural problem – NMR of fluxional molecules – evaluation of rate constants – solid state NMR – magic angle spinning – NMR of paramagnetic molecules – contact shifts and shift reagents – NMR imaging – contrast agents

Unit 4: EPR Spectroscopy
Principle of EPR – instrumentation   – total spin Hamiltonian – presentation of the spectrum – hyperfine splitting – super hyperfine structure –  EPR of hydrogen atom –  splitting in isotropic systems involving more than one nucleus –  EPR spectra of free radicals in solution –  methyl radical, benzene anion, p-benzosemiquinone radical anion, p- nitrobenzoate dianion and naphthalene anion – Evaluation of g and A tensors – factors affecting the magnitude of g values –– anisotropy – zero field splitting – Kramer’s theory & degeneracy – triplet EPR

Unit 5: Application and advanced EPR
Application of EPR in transition metal complexes VO2+ Fe3+ Co2+ ,Mn2+, Ni2+ and bis-salicylaldimine copper (II) – Jahn-Teller theory & distortion studies in Cu(II) complexes – evaluation of spin – orbital coupling constant – exchange coupled EPR  –  multi resonance EPR  – cw and pulsed EPR and ENDOR – MEMS and DAVIS – ESEEM – HYSCORE  –  ESR Spin trapping –  spin labeling – EPR imaging