Sphingolipid Metabolism: Biosynthesis: R.C. Dickson, R.L. Lester, and M. Marek-Nagiec, Serine Palmitoyltransferase. M.M. Grilley and J. Y. Takemoto, Assay of the Saccharomyces cerevisiae Dihydrosphingosine C-4 Hydorxylase. B. Caligan, K. Peters. J. Ou, E. Wang, and A.H. Merrill, Jr., Ceramide Synthase. H. Schulze, C. Michel, and G. van Echten-Deckert, Dihydroceramide Desaturase. M.N. Nikolava-Karakashian, Assays for the Synthesis of Sphingomyelin (Ceramide Phosphocholine) and Ceramide Phosphoethanolamine. J.A. Shayman and A. Abe, Glucosylceramide Synthase: Assay and Properties. P. Marks, Y. Kamisaka, and R.E. Pagano, Methods for Studying Glucosylceramide Synthase. H. Sprong, G. van Meer, and P. van der Sluijs, UDP-Galactose: Ceramide Galactosyltransferase. S. Chatterjee, Assay of Lactosylceramide Synthase and Comments on Its Potential Role in Signal Transduction. G. Pohlentz, C. Kaes, and K. Sandhoff, In Vitro Assays for Enzymes of Ganglioside Synthesis. F.B. Jungalwala, P. Chatturvedi, D.S. Newburg, and M.R. Natowicz, Analyses of Sulfatides and Enzymes of Sulfatide Metabolism. J.A. Shayman and A. Abe, 1-O-Acyleceramide Synthase. T-C. Lee, N-Acetylation of Shingosine by Platelet-Activating Factor: Sphingosine Transacetylase. A.S. Fischl, Y. Liu, A. Browdy, and A.E. Cremesti, Inositolphosphoryl Ceramide Synthase from Yeast. D.V. Lynch, Enzymes of Shingolipid Metabolism in Plants. Turnover: S. Lansmann, O. Bartelsen, and K. Sandhoff, Purification and Characterization of Recombinant Human Acid Sphingomyelinase. B. Liu and Y.A. Hannun, Purification of Rat Brain Membrane Neutral Sphingomyelinase. B.Liu and Y.A. Hannun, Sphingolmyelinase Assay using Radiolabeled Substrates. A.G. Barbone, A.C. Jackson, D.M. Ritchie, and D.C. Argentieri, Robot
Sphingolipids are found in all eukaryotic and in some prokaryotic organisms and provide structure for cell membranes, lipoproteins, and other biological materials as well as participate in the regulation of cell growth, differentiation, and diverse cell functions, including cell-cell communication, cell-substratum interactions, and intracellular signal transduction. This volume presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs. Its companion Volume 312 will contain information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Biochemists, molecular biologists, cell biologists, biomedical researchers, microbiologists, and developmental biologists.
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- © Academic Press 2000
- 22nd October 1999
- Academic Press
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Emory University, Atlanta, Georgia, U.S.A.
Medical University of South Carolina, Charleston, U.S.A.
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA