Some Biological Techniques in Electron Microscopy

Some Biological Techniques in Electron Microscopy

1st Edition - January 1, 1970

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  • Editor: D.F. Parsons
  • eBook ISBN: 9780323160353

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Description

Some Biological Techniques in Electron Microscopy discusses the problems that hinder or prevent progress in biological electron microscopy. This book explores the great potential of electron microscope for solving several pressing medical and biological research problems. Organized into five chapters, this book starts with an overview of the primary goal of biological electron microscopy, which is the visualization of atoms in biological molecules and structures. This text proceeds with a discussion of the freezing methods of electron microscopy in which some of the artifacts producing steps of environmental techniques are eliminated. Other chapters consider the objective of electron microscopy to resolve successively small objects, in the limiting instance, single atoms in amorphous structures. The final chapter deals with processing tissues for electron microscopy wherein temperature, agitation, pressure, and humidity can be controlled throughout the operation. Biologists, biophysicists, physicists, electron microscopists, and medical researchers will find this book extremely useful.

Table of Contents


  • List of Contributors

    Preface

    I. Problems in High Resolution Electron Microscopy of Biological Materials in Their Natural State

    I. Introduction

    II. Resolution Limitations of Present Day Electron Microscopes

    III. Biological High Voltage (1 MeV) Electron Microscopy and Reduction of Specimen Damage

    IV. New Methods of Obtaining Contrast

    V. Conclusions

    References

    II. Chemical Effects of Fixation on Biological Specimens

    I. Introduction

    II. Osmium Tetroxide Fixation

    III. Permanganate Fixation

    IV. Recent Techniques of Specimen Preparation

    References

    III. Present Status of Freezing Techniques

    I. Introduction

    II. Physical Basis of Freezing Techniques

    III. Processing for Examination

    IV. Conclusions

    References

    IV. Reducing the Effect of Substrate Noise in Electron Images of Biological Objects

    I. Introduction

    II. Effect of Substrate on Image

    III. Effect of Beam Potential

    IV. Substrate Structure

    V. Self-Supporting Films

    VI. Ideal Substrate

    VII. Optical Means for Removing Noise

    VIII. Conclusion

    References

    V. Automation in Tissue Processing for Electron Microscopy

    I. Tissue Processors

    II. Fixation, Dehydration, and Embedding

    III. Future Prospects

    References

    Author Index

    Subject Index

Product details

  • No. of pages: 198
  • Language: English
  • Copyright: © Academic Press 1970
  • Published: January 1, 1970
  • Imprint: Academic Press
  • eBook ISBN: 9780323160353

About the Editor

D.F. Parsons

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