Some Biological Techniques in Electron Microscopy - 1st Edition - ISBN: 9780125455503, 9780323160353

Some Biological Techniques in Electron Microscopy

1st Edition

Editors: D.F. Parsons
eBook ISBN: 9780323160353
Imprint: Academic Press
Published Date: 1st January 1970
Page Count: 198
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Some Biological Techniques in Electron Microscopy discusses the problems that hinder or prevent progress in biological electron microscopy. This book explores the great potential of electron microscope for solving several pressing medical and biological research problems. Organized into five chapters, this book starts with an overview of the primary goal of biological electron microscopy, which is the visualization of atoms in biological molecules and structures. This text proceeds with a discussion of the freezing methods of electron microscopy in which some of the artifacts producing steps of environmental techniques are eliminated. Other chapters consider the objective of electron microscopy to resolve successively small objects, in the limiting instance, single atoms in amorphous structures. The final chapter deals with processing tissues for electron microscopy wherein temperature, agitation, pressure, and humidity can be controlled throughout the operation. Biologists, biophysicists, physicists, electron microscopists, and medical researchers will find this book extremely useful.

Table of Contents

List of Contributors


I. Problems in High Resolution Electron Microscopy of Biological Materials in Their Natural State

I. Introduction

II. Resolution Limitations of Present Day Electron Microscopes

III. Biological High Voltage (1 MeV) Electron Microscopy and Reduction of Specimen Damage

IV. New Methods of Obtaining Contrast

V. Conclusions


II. Chemical Effects of Fixation on Biological Specimens

I. Introduction

II. Osmium Tetroxide Fixation

III. Permanganate Fixation

IV. Recent Techniques of Specimen Preparation


III. Present Status of Freezing Techniques

I. Introduction

II. Physical Basis of Freezing Techniques

III. Processing for Examination

IV. Conclusions


IV. Reducing the Effect of Substrate Noise in Electron Images of Biological Objects

I. Introduction

II. Effect of Substrate on Image

III. Effect of Beam Potential

IV. Substrate Structure

V. Self-Supporting Films

VI. Ideal Substrate

VII. Optical Means for Removing Noise

VIII. Conclusion


V. Automation in Tissue Processing for Electron Microscopy

I. Tissue Processors

II. Fixation, Dehydration, and Embedding

III. Future Prospects


Author Index

Subject Index


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© Academic Press 1970
Academic Press
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About the Editor

D.F. Parsons

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