The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volumehas been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 275 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Solid-phase peptide synthesis * Applications of peptides for structural and biological studies * Characterization of synthetic peptides


Biochemists, molecular biologists, immunologists, cell biologists, pharmacologists, and biomedical researchers.

Table of Contents

B. Merrifield, Concept and Early Development of Solid-Phase Peptide Synthesis. Methods for Solid-Phase Assembly of Peptides: P.F. Alewood, D. Alewood, L. Miranda, S. Love, W. Meutermans, and D. Wilson, Rapid in Situ Neutralization Protocols for Boc and Fmoc Solid-Phase Chemistries. J.M. Stewart, Cleavage Methods Following Boc-Based Solid-Phase Peptide Synthesis. D.A. Wellings and E. Atherton, Standard Fmoc Protocols. C.G. Fields and G.B. Fields, Trifluoroacetic Acid Cleavage and Deprotection of Resin-Bound Peptides Following Synthesis by Fmoc Chemistry. M. Meldal, Properties of Solid Supports. F. Albericio and L.A. Carpino, Coupling Reagents and Activation. M.F. Songster and G. Barany, Handles in Solid-Phase Peptide Synthesis. C. Blackburn and S.A. Kates, Solid-Phase Synthesis of Cyclic Homodetic Peptides. I. Annis, B. Hargittai, and G. Barany, Disulfide Bond Formation in Peptides. J. Kihlberg, M. Elofsson, and L.A. Salvador, Direct Synthesis of Glycosylated Amino Acids from Carbohydrate Peracetates and Fmoc Amino Acids: Solid-Phase Synthesis of Biomedicinally Interesting Glycopeptides. J.W. Perich, Synthesis of Phosphopeptides Using Modern Chemical Approaches. S.B.H. Kent, Chemoselective Ligation. A.C. Braisted, J.K. Judice, and J.A. Wells, Synthesis of Proteins by Subtiligase. F. Albericio, P. Lloyd-Williams, and E. Giralt, Convergent Solid-Phase Synthesis. M. Lebl and V.Krchnak, Peptide Libraries. Analytical Techniques: G.A. Grant, M.W. Crankshaw, and J. Gorka, Edman Sequencing as Tool for Characterization of Synthetic Peptides.


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© 1997
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY