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Small GTPases and Their Regulators, Part C: Proteins Involved in Transport - 1st Edition - ISBN: 9780121821586, 9780080883700

Small GTPases and Their Regulators, Part C: Proteins Involved in Transport, Volume 257

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: W. Balch Channing Der Alan Hall
eBook ISBN: 9780080883700
Imprint: Academic Press
Published Date: 30th August 1995
Page Count: 360


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Table of Contents

Expression, Purification, and Posttranslational Modification: C. Nuoffer, F. Peter, and W.E. Balch, Purification of His6-Tagged Rab1 Proteins Using Bacterial and Insect Cell Expression Systems. H. Horiuchi,O. Ullrich, C. Bucci, and M. Zerial, Purification of Posttranslationally Modified and Unmodified Rab5 Protein Expressed in Spodoptera frugiperda Cells. M.A. Riederer, T. Soldati, A.B. Dirac-Svejstrup, and S.R. Pfeffer, Expression of Rab9 Protein in Escherichia coli: Purification and Isoprenylation in Vitro. Y. Jiang, G. Rossi, and S. Ferro-Novick, Characterization of Yeast Type-II Geranylgeranyltransferase. S.A. Armstrong, M.S. Brown, J.L. Goldstein, and M.C. Seabra, Preparation of Recombinant Rab Geranylgeranyltransferase and Rab Escort Proteins. K. Kimura, T. Oka, and A. Nakano, Purification and Assay of Yeast Sar1p. T. Rowe and W.E. Balch, Expression and Purification of Mammalian Sar1. Guanine NucleotideExchange and Hydrolysis: A. Kikuchi, H. Nakanishi, and Y. Takai, Purification and Properties of Rab3A. T. Sasaki and Y. Takai, Purification and Properties of Bovine Rab-GDP Dissociation Inhibitor. F. Peter, C. Nuoffer, I. Schalk, and W.E. Balch, Expression and Purification of Recombinant His6-Tagged Guanine Nucleotide Dissociation Inhibitor and Formation of Rab1 Complex. D.M. Roberts, M.D. Garrett, and P.J. Novick, Purification of GDP Dissociation Stimulator Dss4 from Recombinant Bacteria. J.L. Burton and P. De Camilli, Expression, Purification, and Functional Assay of Mss4. C. Barlowe and R. Schekman, Expression, Purification, and Assay of Sec12p: A Sar1p-Specific GDP Dissociation Stimulator. I.G. Macara and W.H. Brondyk, Oligonucleotide Mutagenesis of Rab GTPases. P. Vollmer and D. Gallwitz, High Expression Cloning, Purification, and Assay of Ypt-GTPase Activating Proteins. P.A. Randazzo, O. Weiss, and R.A. Kahn, Preparation of Recombinant ADP-Ribosylation Factor. F.R. Bischoff and H. Ponstingl, Catalysis of Guanine Nucleotide Exchange of Ran by RCC1 and Stimulation of Hydrolysis of Ran-Bound GTP by Ran-GAP1. T. Yeung, T. Yoshihisa, and R. Schekman, Purification of Sec23p-Sec24p Complex. Cell Expression and Analysis in Vivo: H. Stenmark, C. Bucci, and M. Zerial, Expression of Rab GTPase Using Recombinant Vaccinia Viruses. C. Dascher, E.J. Tisdale, and W.E. Balch, Transient Expression of Small GTPases to Study Protein Transport along Secretory Pathway in Vivo Using the Recombinant T7 Vaccinia Virus System. C. Dascher, J.K. VanSlyke, L. Thomas, W.E. Balch, and G. Thomas, Preparation of Recombinant Vaccinia Virus for Expression of Small GTPases. L. Martin-Parras and M. Zerial, Using Oligonucleotides for Cloning Rab Proteins by PCR. W.H. Brondyk and I.G. Macara, Use of Two-Hybrid System to Identify Rab-Binding Proteins. H. Damke, M. Gossen, S. Freundlieb, H. Bujard, and S.L. Schmid, Tightly Regulated and Inducible Expression of Dominant Interfering Dynamin Mutant in Stably Transformed HeLa Cells. R.W. Holz, R.A. Senter, and M.D. Uhler, Investigation by Transient Transfection of the Effects on Regulated Exocytosis of Rab3A. M.D. Garrett and P.J. Novick, Expression, Purification, and Assays of Gdi1p from Recombinant Escherichia coli. Biological Activity: O. Ullrich, H. Horiuchi, K. Alexandrov, and M. Zerial, Use of Rab-GDP Dissociation Inhibitor for Solubilization and Delivery of Rab Proteins to Biological Membranes in Streptolysin O-Permeabilized Cells. T. Soldati, A.D. Shapiro, and S.R. Pfeffer, Reconstitution of Rab9 Endosomal Targeting and Nucleotide ExchangeUsing Purified Rab9-GDP Dissociation Inhibitor Complexes and Endosome-Enriched Membranes. J.M. McCaffery and M.G. Farquhar, Localization of GTPases by Indirect Immunofluorescence and Immunoelectron Microscopy. F. Melchior, D.J. Sweet, and L. Gerace, Analysis of Ran/Tc4 Function in Nuclear Protein Import. H. Shirataki and Y. Takai, Purification and Properties of Rabphilin-3A. L.A. Huber, P. Dupree, and C.G. Dotti, Use of Antisense Oligonucleotides to Study Rab Function in Vivo. H.A. Brown and P.C. Sternweis, Stimulation of Phospholipase D by ADP-Ribosylation Factor. C. Murphy and M. Zerial, Expression of Rab Proteins during Mouse Embryonic Development. Author Index. Subject Index.


General Description of the Volume: Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport. This volume plus its companion Volumes 255 and 256 cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level. Volume 257 provides detailed protocols to effectively produce, modify, and assay for the function of small GTPases involved in vesicular traffic through the secretory pathway of eukaryotic cells. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Assays to measure GTP exchange and hydrolysis

  • Expression and purification of small GTPases
  • Posttranslational modifications of Rab proteins
  • Generation of trans dominant mutants
  • Expression and purification of Rab effector proteins


Biochemists, molecular biologists, cell biologists, pharmacologists, neurophysiologists, and neurochemists.


No. of pages:
© Academic Press 1995
30th August 1995
Academic Press
eBook ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

W. Balch

Affiliations and Expertise

The Scripps Research Institute, La Jolla, CA, USA

Channing Der

Affiliations and Expertise

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA

Alan Hall

Affiliations and Expertise

University College of London, U.K.