Small GTPases and Their Regulators, Part B: Rho Family, Volume 256

1st Edition

Serial Volume Editors: W. Balch Channing Der Alan Hall
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821579
eBook ISBN: 9780080883694
Imprint: Academic Press
Published Date: 15th August 1995
Page Count: 401


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Table of Contents

Expression, Purification, and Posttranslational Modification:

A.J. Self and A. Hall, Purification of Recombinant Rho/Rac/G25K fromEscherichia coli.

R.A.Cerione, D. Leonard, and Y. Zheng, Purification of Baculovirus-Expressed Cdc42Hs.

T. Mizuno, H. Nakanishi, and Y. Takai, Purification and Properties of Rac2 from Human Leukemia Cells.

U.G. Knaus and G.M. Bokoch, Purification of Rac2 from Human Neutrophils.

A. Abo, Purification of Rac(GDI Dissociation Inhibitor Complex from Phagocyte Cytosol.

K. Tanaka, T. Sasaki, and Y. Takai, Purification and Properties of Recombinant Rho(GDP Dissociation Inhibitor.

M.R. Philips and M.H. Pillinger, Prenylcysteine-Directed Carboxyl Methyltransferase Activity in Human Neutrophil Membranes. Guanine Nucleotide Exchange and Hydrolysis:

A.J. Self and A. Hall, Measurement of Intrinsic Nucleotide Exchange and GTP Hydrolysis Rates.

Y. Zheng, M.J. Hart, and R.A. Cerione, Guanine Nucleotide Exchange Catalyzed by dbl Oncogene Product.

E. Porfiri and J.F. Hancock, Stimulation of Nucleotide Exchange on Ras- and Rho-Related Proteins by Small GTP-Binding Protein GDP Dissociation Stimulator.

T. Miki, Interaction of Ect2 and Dblwith Rho-related GTPases.

D.A. Leonard and R.A. Cerione, Solubilization of Cdc42Hs from Membranes by Rho(GDP Dissociation Inhibitor.

J. Settleman and R. Foster, Purification and GTPase-Activating Protein Activity of Baculovirus Expressed p190.

S. Ahmed, R. Kozma, C. Hall, and L. Lim, GTPase-Activating Protein Activity of n(alpha1)-Chimaerin and Effect of Lipids.

D.E.H. Afar and O.N. Witte, Characterization of Breakdown Cluster Region Kinase an


General Description of the Volume: Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport. This volume plus its companion Volumes 255 and 257 cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level. It is the first compendium of practical techniques for working with small GTPases of the Rho group.

General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Researchers in biochemistry, molecular biology, cell biology, pharmacology, neurophysiology, and neurochemistry.


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© Academic Press 1995
Academic Press
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About the Serial Volume Editors

W. Balch Serial Volume Editor

Affiliations and Expertise

The Scripps Research Institute, La Jolla, CA, USA

Channing Der Serial Volume Editor

Affiliations and Expertise

Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA

Alan Hall Serial Volume Editor

Affiliations and Expertise

University College of London, U.K.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA