Expression, Purification, and Posttranslational Modification:
S.L. Campbell-Burk and J.W. Carpenter, Refolding and Purification of Ras Proteins.
E. Porfiri, T. Evans, G.E. Bollag, R. Clark, and J.F. Hancock, Purification of Baculovirus-Expressed Recombinant Ras and Rap Proteins.
G.E. Bollag and F. McCormick, Purification of Recombinant Ras GTPase-Activating Proteins.
B. Rubinfeld and P. Polakis, Purification of Baculovirus-Produced Rap 1 GTPase-Activating Proteins.
G.L. James, M.S. Brown, and J.L. Goldstein, Assays for Inhibitors for CAAX Farnesyltransferase in Vitro and in Intact Cells.
R. Khosravi-Far and C.J. Der, Prenylation Analysis of Bacterially Expressed and Insect Cell-Expressed Ras and Ras-Related Proteins.
J.F. Hancock, Reticulocyte Lysate Assay for in Vitro Translation and Posttranslational Modification of Ras Proteins.
C. Volker and J.B. Stock, Carboxyl Methylation of Ras-Related Proteins.
F. Tamanoi and H. Mitsuzawa, Use of Yeast for Identification of Farnesyltransferase Inhibitors and for Generation of Mutant Farnesyltransferases.
Guanine Nucleotide Exchange and Hydrolysis:
C. Lenzen, R.H. Cool, and A. Wittinghofer, Analysis of Intrinsic and CDC25-Stimulated Guanine Nucleotide Exchange of p21ras--Nucleotide Complexes by Fluorescence Measurements.
J. Downward, Measurement of Nucleotide Exchange and Hydrolysis Activities in Immunoprecipitates.
J.B. Gibbs, Determination of Guanine Nucleotides Bound to Ras in Mammalian Cells.
M. Frech, D. Cussac, P. Chardin, and D. Bar-Sagi, Purification of Baculovirus-Expressed Human Sos1 Protein.
M. Hart and S. Powers, Ras-Cdc25 and R