General Description of the Volume: Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport. This volume plus its companions Volumes 256 and 257 cover all the biochemical and biological assays currently in use for analyzing the role of small GTPases in many aspects of cell biology at the molecular level.
General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
@introbul:Key Features @bul:* Expression, purification, and posttranslational modification
- Guanine nucleotide exchange and hydrolysis
- Cell expression and analysis in Vitro
- Biological activity
Biochemists, molecular biologists, cell biologists, pharmacologists, neuropsychologists, and neurochemists.
Expression, Purification, and Posttranslational Modification:
S.L. Campbell-Burk and J.W. Carpenter, Refolding and Purification of Ras Proteins.
E. Porfiri, T. Evans, G.E. Bollag, R. Clark, and J.F. Hancock, Purification of Baculovirus-Expressed Recombinant Ras and Rap Proteins.
G.E. Bollag and F. McCormick, Purification of Recombinant Ras GTPase-Activating Proteins.
B. Rubinfeld and P. Polakis, Purification of Baculovirus-Produced Rap 1 GTPase-Activating Proteins.
G.L. James, M.S. Brown, and J.L. Goldstein, Assays for Inhibitors for CAAX Farnesyltransferase in Vitro and in Intact Cells.
R. Khosravi-Far and C.J. Der, Prenylation Analysis of Bacterially Expressed and Insect Cell-Expressed Ras and Ras-Related Proteins.
J.F. Hancock, Reticulocyte Lysate Assay for in Vitro Translation and Posttranslational Modification of Ras Proteins.
C. Volker and J.B. Stock, Carboxyl Methylation of Ras-Related Proteins.
F. Tamanoi and H. Mitsuzawa, Use of Yeast for Identification of Farnesyltransferase Inhibitors and for Generation of Mutant Farnesyltransferases.
Guanine Nucleotide Exchange and Hydrolysis:
C. Lenzen, R.H. Cool, and A. Wittinghofer, Analysis of Intrinsic and CDC25-Stimulated Guanine Nucleotide Exchange of p21ras--Nucleotide Complexes by Fluorescence Measurements.
J. Downward, Measurement of Nucleotide Exchange and Hydrolysis Activities in Immunoprecipitates.
J.B. Gibbs, Determination of Guanine Nucleotides Bound to Ras in Mammalian Cells.
M. Frech, D. Cussac, P. Chardin, and D. Bar-Sagi, Purification of Baculovirus-Expressed Human Sos1 Protein.
M. Hart and S. Powers, Ras-Cdc25 and R
- No. of pages:
- © Academic Press 1995
- 4th August 1995
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Volume @qu:"These books are a most useful and valuable resource to everyone involved in the field of protein research. They will certainly serve as guidance books and many of the techniques described might remain central to the field of signal transduction in the future." @source:--Tilat A. Rizvi, University of Cincinnati Medical Center, in TINS @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY