Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.

Key Features

* Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools
* Methods included are useful to both established researchers and newcomers to the field
* Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines


Biochemists, geneticists, molecular biologists, cell biologists, and biophysicists.

Table of Contents

1. Watching single DNA replication loops under flow extension

Antoine Van Oijen

2. Star polymer surface passivation for single molecule detection

Jürgen Groll , Martin Möller

3. Ultrahigh resolution detection of single active motor proteins in live cells

Paul Selvin, Erdal Toprak & Comert Kural

4. Molecules and Methods for Superresolution Imaging

W.E. Moerner, Michael Thompson, Sam Lord, Julie Biteen, and Nick Conley +…

5. Aqueous nanodroplets for studying single molecules

Lori Goldner

6. High-speed atomic force microscopy techniques for visualizing dynamic behavior of biological macromolecules

Toshio Ando

7. Single-Biomolecule Spectroscopy Using Microfluidic Platforms

Richard Zare, Dr. Olga Dudko, Dr. Jérôme Mathé

8. DNA Looping Kinetics Analyzed by Tethered Particle Microscopy

Laura Finzi

9. Single molecule observation of proteins in vivo

Shimon Weiss, Gopal Iyer

10. DNA curtains as a high-throughput approach to single molecule imaging

Eric Greene

11. Single-molecule enzymology of protein synthesis

Ruben Gonzalez

12. Single molecule fluorescence studies of intrinsically disordered proteins

Ashok Deniz

13. Nanovesicle trapping for studying transient protein-protein interactions by single molecule FRET

Peng Chen

14. Tracking single motor proteins in the cytoplasm of mammalian cells

Edgar Meyhöfer

15. Conformational States of F1-ATPase by Single-Molecule Rotation

Hiroyuki Noji

16. Single Molecule Sequencing by Fluorescence Imaging

Patrice Milos

17. Real-Time DNA Sequencing from Single Polymerase Molecules

Jonas Korlach

18. Micropatterning and single molecule imaging for quantitative analysis of protein-protein interactions in living cells

Gerhard Schütz

19. Probing virus-recep


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© 2010
Academic Press
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