Sertoli Cell Biology

Sertoli Cell Biology

2nd Edition - November 19, 2014

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  • Editor: Michael Griswold
  • Hardcover ISBN: 9780124170476
  • eBook ISBN: 9780124170483

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Sertoli Cell Biology, Second Edition summarizes the progress since the last edition and emphasizes the new information available on Sertoli/germ cell interactions. This information is especially timely since the progress in the past few years has been exceptional and it relates to control of sperm production in vivo and in vitro.

Key Features

  • Fully revised
  • Written by experts in the field
  • Summarizes 10 years of research
  • Contains clear explanations and summaries
  • Provides a summary of references over the last 10 years


Research scientists in the field of reproductive biology, urologists, andrologists, and endocrinologists

Table of Contents

  • List of contributors
    1. Sertoli cell anatomy and cytoskeleton
    I Introduction
    II Sertoli cell morphology
    III Sertoli cell cytoskeleton
    IV Concluding remarks

    2. Establishment of fetal Sertoli cells and their role in testis morphogenesis
    I Introduction
    II Establishment of the gonadal primordium
    III Sertoli cell specification and diversion of molecular development toward the testis pathway
    IV Organizational functions of fetal Sertoli cells

    3. Early postnatal interactions between Sertoli and germ cells
    I Introduction
    II Neonatal testis development
    III Role of Sertoli cells in gonocyte proliferation and migration
    IV Role of Sertoli cells in formation of primary undifferentiated and differentiating spermatogonial populations
    V Concluding remarks

    4. The spermatogonial stem cell niche in mammals
    I Research advances related to the mammalian SSC niche since 2003
    II Principles of stem cell niches in mammalian tissues
    III Spermatogonial stem cells
    IV Location of the SSC
    V Factors governing SSC self-renewal and differentiation
    VI The environment inside and outside the niche
    VII The role of cell migration in SSC self-renewal and differentiation
    VIII Spermatogonial differentiation
    IX Niche localization: what controls the controller?
    X The SSC niche and the cycle of the seminiferous epithelium
    XI The SSC niche during cell loss
    XII Perspectives

    5. DMRT1 and the road to masculinity
    I Introduction
    II DMRT1 expression
    III Regulation of DMRT1
    IV DMRT1 locus and gene expression
    V DMRT1 function
    VI Conclusions

    6. Hormonal regulation of spermatogenesis through Sertoli cells by androgens and estrogens
    I Introduction
    II Androgen signaling
    III Testosterone production and action
    IV Androgen receptor
    V The role of androgens in Sertoli cells
    VI AR-dependent gene expression in Sertoli cells
    VII Sertoli cell estrogen signaling (from androgens via aromatase)
    VIII Conclusions and future perspectives

    7. Activins and inhibins in Sertoli cell biology: Implications for testis development and function
    I Introduction: activin and inhibin link multiple cell types to determine male reproductive health
    II General structure and signaling pathways
    III Regulation of inhibin and activin production
    IV Activin and inhibin function in the adult testis
    V Activin and inhibin in the developing testis
    VI The contribution of Smads to regulation of testis development and growth
    VII Clinical relevance of activin and inhibin for male reproduction
    VIII Concluding remarks: the need to understand signaling crosstalk in the testis

    8. The initiation of spermatogenesis and the cycle of the seminiferous epithelium
    I Introduction and highlights since the last volume
    II Differentiation of spermatogonia
    III Evidence that RA is required for the initiation of meiosis
    IV The initiation of asynchronous spermatogenesis by RA
    V Regulation of RA synthesis and degradation in the developing testis
    VI Extrinsic versus intrinsic factors

    9. Retinoic acid metabolism, signaling, and function in the adult testis
    I Introduction
    II RA synthesis, signaling, and degradation
    III Components of the RA pathway within the adult testis
    IV Maintenance of the spermatogenic cycle by RA
    V Sertoli cell contributions to RA function within the adult testis
    VI The effects of retinoids on Sertoli cell function
    VII Conclusions and remaining questions

    10. Stage-specific gene expression by Sertoli cells
    I Introduction
    II Evidence that spermatogenic cells regulate biologically important, stage-specific functions of Sertoli cells
    III CTSL, a model for the analysis of the function and regulation of stage-specific gene expression
    IV Stage-specific gene expression as a fundamental characteristic of Sertoli cells
    V Future directions

    11. MicroRNAs and Sertoli cells
    I Noncoding RNAs
    II miRNAs
    III The role of miRNAs in spermatogenesis in vivo
    IV SC-expressed miRNAs and their functions
    V Regulation of SC-expressed miRNAs
    VI Perspective

    12. Biochemistry of Sertoli cell/germ cell junctions, germ cell transport, and spermiation in the seminiferous epithelium
    I Introduction
    II Cell junctions and their restructuring during the epithelial cycle in the testis
    III Ectoplasmic specialization
    IV Spermatid transport and spermiation
    V Transport of preleptotene spermatocytes at the BTB
    VI Concluding remarks and future perspectives

    13. Sertoli cell structure and function in anamniote vertebrates
    I Introduction
    II Sertoli cell proliferation
    III Sertoli cell functions
    IV Concluding remarks

    14. Adult Sertoli cell differentiation status in humans
    I Introduction and scope of the chapter
    II Development of the adult Sertoli cell population
    III Proliferation and differentiation around puberty
    IV Differentiation in adult life
    V Human Sertoli cell differentiation and pathology
    VI Future perspectives

    15. Gene knockouts that affect Sertoli cell function
    I Introduction
    II Genes identified as essential for normal Sertoli cell development and function through KO studies
    III Lessons learned from the gene KO studies
    IV Approaches to gene ablation in Sertoli cells
    V Conclusions and perspectives

Product details

  • No. of pages: 488
  • Language: English
  • Copyright: © Elsevier 2014
  • Published: November 19, 2014
  • Imprint: Elsevier
  • Hardcover ISBN: 9780124170476
  • eBook ISBN: 9780124170483

About the Editor

Michael Griswold

Michael Griswold

Excerpt from Hartman Award from Society for the Study of Reproduction Carl G. Hartman Award

(Supported by a grant from Cook Medical)

This award is the most prestigious award conferred by the Society for the Study of Reproduction. Each year, this award recognizes an exemplary research career in reproductive biology. The 2013 recipient of the Carl G. Hartman Award is Michael D. Griswold, Ph.D.

Dr. Michael D. Griswold, Regents Professor of Molecular Biosciences at Washington State University (WSU), is world-renowned for his contributions to our understanding of Sertoli cell structure and functions and the crucial role of Vitamin A in spermatogenesis and a loyal member of SSR. Mike received a B.S. in Chemistry and Ph.D. in Biochemistry from the University of Wyoming. He conducted postdoctoral studies with Dr. Philip Cohen at the University of Wisconsin, funded by an NIH Postdoctoral Fellowship, working on amphibian metampophosis, and with Dr. Flauco P. Tocchini-Valentini in Rome, funded by an EMBO Fellowship, on Xenopus RNA. Mike spent a year as an Assistant Professor of Pharmacology at Baylor College of Medicine in Houston. He then spent two years as a Research Associate in the laboratory of Dr. Irving Fritz at the University of Toronto, where he acquired what would become a life-long interest in Sertoli cells. Mike joined the faculty of WSU in 1976 as an Assistant Professor of Biochemistry and Basic Medical Sciences, was promoted to Associate and then full Professor, appointed Chair of the Department of Biochemistry and Biophysics in 1994, Director of the School of Molecular Biosciences in 1999, and Dean of the College of Sciences, a position he held from 2003-2010. Mike became a Regents Professor of Molecular Biosciences in 2008, and in 2011, received the WSU Eminent Faculty Award, the highest faculty honor at WSU.

Mike’s research has been continuously funded by the NIH since 1977 (with the same grant “Hormonal Control of the Maturation of Sertoli Cells”), including a Research Career Develop Award (1979-1984) and MERIT Award (1997-2007). He has published more than 230 original scientific articles, book chapters, and review articles. His recognition in the field of Sertoli cell function and spermatogenesis is evidenced by his many keynote addresses that include the SSR President’s Symposium (1994 and 2007), Larry Ewing Lecture at Johns Hopkins University (2001), Andrew Nalbandov Lecture at the University of Illinois (1993), Asdell Lecture at Cornell University (2008), the Bailly Lecture at Southern Illinois University (2010), the Serono Lecturer at the Andrology Society (1994), and the Distinguished Faculty Address at WSU (1994). Mike received the WSU Sahlin Excellence in Research Award (1999) and the SSR Research Award (2006), and became an AAAS Fellow in 2009.

Mike is an exemplary mentor. He trained 7 MS students, 24 graduate students that include Mike Skinner, Ken Roberts and Leslie Heckert, and 26 postdoctoral fellows and research associates that include Carlos Morales, Kwan Hee Kim, Carol Linder, Dereck McLean, and Cathryn Hogarth. Mike received the Frontiers in Reproduction Beacon Award in 2008 for his outstanding mentoring. One of his former students, Leslie Heckert wrote: “His high expectations returned dedication, creativity and independence from the members of his laboratory, which in turn produced a vibrant and exciting research environment. He led by example, support and occasionally by providing unsolicited words of encouragement...”.

Mike has unselfishly served his profession, SSR, the Andrology Society, and the Testis Workshop. He was a member of the SSR Board of Directors (1989-1992), SSR Annual Meeting Program Chair (1998), SSR President-Elect and President (1997-1999), Andrology Society Board of Directors (2000-2003), and Program Committee Chair for Testis Workshop (1993 and 2007) and Andrology Society (1996). He served the NIH on the Reproductive Biology Study Section (1983-1987, and continues to do ad hoc reviews for the NIH. Mike has also served as an Editorial Board Member for Endocrinology, Journal of Andrology, Biology of Reproduction, Endocrine Journal, Molecular Cellular Endocrinology, and Spermatogenesis.

Mike’s contributions to Sertoli cell physiology and spermatogenesis are impressive. His lab demonstrated in 1980 that vitamin A modulated Sertoli cell function, in 1987 that vitamin A altered Sertoli cell gene expression, and in 1987 in a landmark publication with Carlos Morales, that retinol induced synchronization of seminiferous tubules in vitamin A-deficient rats. The crucial role that vitamin A plays in regulating germ cell entry into meiosis is still being investigated today not only by the Griswold lab but also by many others in the field, including Kwan Hee Kim, Peter Koopman, and David Page. Moreover, Mike’s leadership role in this area is evidenced by his collaborative publications with John McCarrey, the late Lonnie Russell, Martin Dym, Bob Braun, Kate Loveland, David Zarkower, Marvin Meistrich, and Paul Cooke.

Mike’s lab pioneered microarray/gene profiling analyses of FSH-treated Sertoli cells, beginning in 2002, followed by analysis of spermatogonial stem cells, testis, seminiferous epithelium, epididymis, spermatogenesis, and embryonic development of the testis. These databases are freely available to the scientific community. The number of investigators across the world that have used these data bases is remarkable.

As evidenced from the comments above, Mike has been at the forefront of research in male reproductive biology for the past three decades. Leslie Heckert wrote: “His ideas, approaches, and results have enhanced our understanding of Sertoli cell and gamete function, improved animal models and methodologies for the study of spermatogenesis, and precipitated new concepts in testis biology, contraception and infertility. Because of Dr. Griswold, more is known about the role of retinoic acid in regulating meiosis and the cycle of the seminiferous epithelium, the actions of FSH and testosterone, Sertoli cell function, and the characteristics of spermatogonial stem cells.”

Michael Griswold meets and exceeds the criteria for the Hartman Award in terms of his original research, mentoring of many successful scientists, foresight generosity to the field, and leadership. He has made SSR proud. (Submitted by Mary Hunzicker-Dunn, Ph.D.).

Affiliations and Expertise

School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA, U.S.A.

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