Serology and Immunochemistry of Plant Viruses - 1st Edition - ISBN: 9780127141800, 9780323156325

Serology and Immunochemistry of Plant Viruses

1st Edition

Authors: M.H.V. Van Regenmortel
eBook ISBN: 9780323156325
Imprint: Academic Press
Published Date: 28th February 1982
Page Count: 316
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Description

Serology and Immunochemistry of Plant Viruses investigates the antigenic properties of plant viruses. It looks at the practical aspects of plant virus serology, along with the molecular basis of viral antigenicity, antigenic determinants in proteins, the structure of antibodies, virus purification, antiserum production, and the theoretical principles and practical implementation of the various serological techniques. It also considers the problems associated with identification and classification of plant viruses.
Organized into 10 chapters, this volume begins with an overview of antigens and antigenic determinants before proceeding with a discussion of the immunochemistry of plant viruses, virus-antibody binding, the role of quaternary structure in antigenicity, and the structure of viral antigenic determinants. The reader is also introduced to the methods and principles of purifying plant viruses, preparation of antisera and purification of antibodies, antigen-antibody interaction, immunochemical techniques used with plant viruses, the role of quaternary structure on viral antigenicity, diagnosis of virus diseases, use of serological criteria for measuring the degree of relationship between viruses, and immunochemical studies of plant viruses. The book includes a bibliography with 1,400 references and a list of all the plant viruses that have been studied by serology. This book will be a useful resource for virologists and plant pathologists, as well as for students and research workers in plant virology, plant pathology, microbiology, and general virology.

Table of Contents


Preface

List of Virus Abbreviations

1 Antigens and Antigenic Determinants

A. Definitions

B. Immunogenicity

C. Three-Dimensional Structure of Proteins

D. Antigenic Determinants

E. Antigenic Structure of Myoglobin

F. Antigenic Structure of Lysozyme

2 Antibodies

A. Structure of IgG

B. Properties of the Main Immunoglobulin Classes

C. Functional Properties of Immunoglobulins

3 Virus Purification

A. Introduction

B. Removal of Plant Antigens

C. Clarification

D. Concentration

E. Removal of Residual Impurities

F. Preservation of Virus Material

4 Preparation of Antisera and Purification of Antibodies

A. Preparation of Antisera

B. Purification of Immunoglobulins

C. Purification of Antibody

5 Antigen-Antibody Interaction

A. Forces Involved in Antigen-Antibody Interaction

B. Methods Used for Measuring Antibody Affinity

C. The Law of Mass Action

6 Serological Techniques

A. Precipitin Tests

B. Immunodiffusion

C. Immunoelectrophoresis

D. Agglutination

E. Complement Fixation

F. Labeled Antibody Techniques

G. Tests with Radioisotope-Labeled Viral Antigens

H. Immunoelectron Microscopy

I. Neutralization

7 Role of Quaternary Structure in Viral Antigenicity

A. Soluble Antigens

B. Cryptotopes and Neotopes

C. Studies with Individual Viruses

8 Virus Identification

A. Introduction

B. Diagnosis of Virus Diseases

C. Identification of Individual Viruses

D. Sensitivity of Virus Detection

9 Virus Classification

A. Use of Serological Tests for Determining Relationships between Viruses

B. Aims, Methods, and Uses of Virus Classification

C. Serological Relationships among Members of Recognized Virus Groups

10 Immunochemical Studies of Plant Viruses

A. Elucidation of the Antigenic Structure of Plant Viruses

B. Binding Studies

C. TMV as an Experimental Antigen

D. Viruses as Markers in Electron Microscopy

References

Author Index

Subject Index






Details

No. of pages:
316
Language:
English
Copyright:
© Academic Press 1982
Published:
Imprint:
Academic Press
eBook ISBN:
9780323156325

About the Author

M.H.V. Van Regenmortel