Selenoprotein Structure and Function

Selenoprotein Structure and Function

1st Edition - February 1, 2022
This is the Latest Edition
  • Editor: Eranthie Weerapana
  • Hardcover ISBN: 9780323907354

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Description

Selenoprotein Structure and Function, Volume 662 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Identification of Selenoprotein O substrates using a biotinylated ATP analog, Selenium-encoded isotopic signature targeted profiling, Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reporters, Preparation of selenoprotein S by chemical ligation, Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells, SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyond, Selenocysteine substitutions in thiyl radical enzymes, and much more. Additional chapters cover Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strain, Metabolic labeling with radioactive selenium in zebrafish, Low pH isoTOP-ABPP to identify selenocysteines, Expression of selenoproteins via genetic code expansion in mammalian cells, Alpha-methyl selenocysteine as a tool for the study of selenoproteins, Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on Selenoprotein Structure and Function

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Identification of Selenoprotein O substrates using a biotinylated ATP analog
    Anju Sreelatha
    2. Selenium-encoded isotopic signature targeted profiling
    Chu Wang
    3. Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reporters
    Dieter Söll
    4. Preparation of selenoprotein S by chemical ligation
    Sharon Rozovsky
    5. Examining xCT-mediated selenium uptake and selenoprotein production capacity in cells
    Dohoon Kim
    6. SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyond
    Yaoyang Zhang
    7. Selenocysteine substitutions in thiyl radical enzymes
    Brandon Greene
    8. Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strain
    Elias S.J. Arnér
    9. Metabolic labeling with radioactive selenium in zebrafish
    Paul Copeland
    10. Low pH isoTOP-ABPP to identify selenocysteines
    Daniel Bak
    11. Expression of selenoproteins via genetic code expansion in mammalian cells
    Jennifer Peeler
    12. Alpha-methyl selenocysteine as a tool for the study of selenoproteins
    Robert Hondal
    13. Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications
    Jianguo Fang and Baoxin Zhang
    14. Diselenide-selenoester ligation
    Richard Payne
    15. Modeling selenocysteine-derived reactive intermediates utilizing a molecular cradle
    Kei Goto
    16. Methods for accurate and reproducible studies of pharmacological effects of selenium in cancer
    Mikael Björnstedt

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: February 1, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323907354
  • About the Serial Volume Editor

    Eranthie Weerapana

    Eranthie Weerapana is an Associate Professor of Chemistry at Boston College. She received her B.S. in Chemistry from Yale University, and her Ph.D. in Chemistry from MIT, where she worked with Professor Barbara Imperiali, investigating glycosyltransferases involved in N-linked glycosylation in the gram negative bacterium Campylobacter jejuni. She then performed postdoctoral studies at The Scripps Research Institute, La Jolla where she worked with Professor Benjamin F. Cravatt to develop chemical-proteomic methods to investigate reactive cysteines in complex proteomes. Her interdisciplinary research program focuses on applying mass-spectrometry methods to identify regulatory cysteine residues in the human proteome, and chemical biology approaches to develop covalent small-molecule modulators for cysteine-mediated protein activities.

    Affiliations and Expertise

    Associate Professor of Chemistry Chemistry Department Faculty Morrissey College of Arts and Sciences Boston College