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Selection of the HPLC Method in Chemical Analysis
1st Edition - November 1, 2016
Authors: Serban C. Moldoveanu, Victor David
Language: English
Paperback ISBN:9780128036846
9 7 8 - 0 - 1 2 - 8 0 3 6 8 4 - 6
eBook ISBN:9780128037119
9 7 8 - 0 - 1 2 - 8 0 3 7 1 1 - 9
Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection…Read more
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Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation.
High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection.
For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation.
Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis results
Leads researchers to the best choice of an HPLC method from the overabundance of information existent in the field
Provides criteria for HPLC method selection, development, and validation
Authored by world-renowned HPLC experts who have more than 60 years of combined experience in the field
Analytical chemists practicing HPLC and students at the graduate level taking related coursework in analytical chemistry
Preface
Chapter 1. Start of the Implementation of a New HPLC Method
1.1. Collection of Information and Planning for a New Method
1.2. Overview of an Analytical Technique
1.3. Statistical Evaluation of Data and Criteria for Method Validation
Chapter 2. Short Overviews of Analytical Techniques Not Containing an Independent Separation Step
2.1. Summary Classification of Analytical Techniques Not Containing an Independent Separation
2.2. Optical Techniques
2.3. Mass Spectrometry
2.4. Electrochemical Methods
2.5. Other Analytical Techniques Not Including the Separation of Sample Components
2.6. Selection of a Nonhyphenated Method of Analysis versus one Containing a Separation Step
Chapter 3. Short Overviews of the Main Analytical Techniques Containing a Separation Step
3.1. Separation Types Used in the Core Analytical Techniques
3.2. Gas Chromatography
3.3. Supercritical Fluid Chromatography
3.4. High-Performance Liquid Chromatography
3.5. Electrophoresis and Electrochromatography
3.6. Selection of GC, SFC, CZE, or HPLC
Chapter 4. Basic Information Regarding the HPLC Techniques
4.1. Basic Information About Instrumentation in HPLC
4.2. Parameters Describing the Chromatographic Process
4.3. Retention and Elution Mechanisms in Different Types of HPLC
4.4. The Influence of pH, Temperature, and Additives on Retention Equilibria
Chapter 5. Properties of Analytes and Matrices Determining HPLC Selection
5.1. Physicochemical Properties Related to Separation
5.2. Physicochemical Properties Related to Detection
5.3. Selection of the HPLC Separation Based on Sample Properties
5.4. Selection of the HPLC Separation Based on Analyte Properties
5.5. Selection of the HPLC Separation Depending on the Matrix
5.6. Review of Sample Properties with the Goal of Selection of a Detector in HPLC
Chapter 6. General Aspects Regarding the HPLC Analytical Column
6.1. Construction of the High-Performance Liquid Chromatography (HPLC) Column
6.2. Column Properties Affecting Separation
6.3. General Rules for Column Selection, Utilization, and Care
Chapter 7. RP-HPLC Analytical Columns
7.1. Types of Reversed Phases and Their Preparation
7.2. Properties of Reversed-Phase HPLC Columns
7.3. Parameters Used for the Characterization of Reversed-Phase HPLC Columns
7.4. Common and Special RP Columns
7.5. Selection of an RP-HPLC Column
Chapter 8. Polar Analytical Columns
8.1. Types of Polar HPLC Phases and Their Preparation
8.2. Properties and Characterization of Polar HPLC Phases
8.3. Columns With a Polar Stationary Phase
8.4. Selection of a Polar Column
Chapter 9. Stationary Phases and Columns for Ion Exchange, Ion-Moderated, and Ligand Exchange Chromatography
9.1. Types of Phases and their Preparation
9.2. Characterization of Ion Exchange Phases
9.3. Common Columns with Ion Exchange Phases
9.4. Selection of an Ion Exchange Phase
Chapter 10. Stationary Phases and Columns for Chiral Chromatography
10.1. Types of Chiral Phases and Their Preparation
10.2. Characterization of Chiral Phases
10.3. Selection of a Chiral Phase
Chapter 11. Stationary Phases and Columns for Size Exclusion
11.1. Types of Phases and Their Preparation
11.2. Characterization of Size-Exclusion Phases and Columns
11.3. Selection of Columns Used in SEC
Chapter 12. Stationary Phases and Columns for Immunoaffinity Type Separations
12.1. Types of Phases and Their Preparation
Chapter 13. Solvents, Buffers, and Additives Used in the Mobile Phase
13.1. Characterization of Liquids as Solvents
13.2. Additional Properties of Liquids Affecting Separation
13.3. Properties of Solvents of Importance for Detection
13.4. Buffers and Additives
13.5. Selection of Mobile Phase in HPLC
13.6. Selection of a Solvent for Sample Injection
13.7. Selection of a Solvent for the Needle Wash
Chapter 14. Gradient Elution
14.1. The Use of Gradient in HPLC
14.2. Parameters Characterizing the Gradient Separation
14.3. Selection of Gradient in Different Chromatographic Types
Chapter 15. The Practice of HPLC
15.1. The Development of an HPLC Method
15.2. Special HPLC Techniques
Appendix 1. USP Classification of HPLC Columns
Appendix 2. Hydrophobic Stationary Phases
Appendix 3. HILIC and NPC Stationary Phases
Appendix 4. Ion Exchange and Ion-Moderated Stationary Phases
Appendix 5. Chiral Stationary Phases
Appendix 6. Size-Exclusion Stationary Phases
Appendix 7. Properties of Mobile Phase Components
Index
No. of pages: 598
Language: English
Edition: 1
Published: November 1, 2016
Imprint: Elsevier
Paperback ISBN: 9780128036846
eBook ISBN: 9780128037119
SM
Serban C. Moldoveanu
Dr. Serban C. Moldoveanu is Senior Principal Scientist at R. J. Reynolds Tobacco Company. His research activity is focused on various aspects of chromatography including method development for the analysis by GC/MS, HPLC, and LC/MS/MS of natural products and cigarette smoke. He has also performed research on pyrolysis of a variety of polymers and small molecules. He has over 100 publications in peer reviewed journals, eleven books, and several chapter contributions. He is a member of the editorial board of the Journal of Analytical Methods in Chemistry.
Affiliations and expertise
Senior Principal Scientist, RJ Reynolds Tobacco Co., Winston-Salem, NC, USA
VD
Victor David
Dr. Victor David is a Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania. He is the author of more than 120 publications, including 92 scientific papers in ISI international journals and 10 books and chapters in various Encyclopedias. He is also a reviewer at several international journals.
Affiliations and expertise
Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania
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