Schering Workshop on Steroid Hormone 'Receptors', Berlin, December 7 to 9, 1970

Schering Workshop on Steroid Hormone 'Receptors', Berlin, December 7 to 9, 1970

Advances in the Biosciences

1st Edition - January 1, 1971

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  • Editor: Gerhard Raspé
  • eBook ISBN: 9781483160023

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Description

Advances in the Biosciences 7: Schering Workshop on Steroid Hormone ""Receptors,"" Berlin, December 7 to 9, 1970 is a collection of papers presented at the Schering Workshop on Steroid Hormone ""Receptors,"" held in Berlin, Germany, on December 7-9, 1970. Contributors review research findings concerning steroid hormone receptors and cover topics organized around receptors of estrogen, androgen, progesterone, aldosterone, and corticosteroids. This book is comprised of 20 chapters and begins by analyzing the concentration of the estrogen binding protein in the rat uterus in three stages of uterine development, followed by a discussion on estradiol binding in mammalian tissues. The next section explores androgen receptors and includes chapters dealing with the specific binding of steroid-receptor complexes to DNA as well as the effects of androgen receptors on rat and human prostate. Subsequent chapters discuss the action of progesterone, aldosterone, and corticosteroid receptors. This monograph will be of interest to biochemists, biologists, and physiologists.

Table of Contents

  • Opening Address

    Introduction

    I: Estrogen Receptors

    Chapter 1: The Regulation of Uterine Concentration of Estrogen Binding Protein

    Summary

    Introduction

    Ontogeny of the estrogen binding protein Period 1: The development of uterine binding

    Period 2: Equilibrium

    Period 3: Depletion-replenishment cycle after estrogen

    Final Remarks

    Acknowledgment

    Discussion

    Chapter 2: Studies on Estradiol-binding in Mammalian Tissues

    Summary

    Introduction

    Discussion

    Chapter 3: Estradiol Receptors in the Uterus

    Summary

    Introduction

    Techniques

    Early findings: in vivo and tissue in vitro

    Cytosol binding proteins Intrinsic vs possible extrinsic specificity

    Conformation

    Binding parameters

    Nuclear receptors “Neo-nuclear” receptor

    Non-Histone Chromatin estradiol protein

    Receptor and RNA Synthesis

    The least complicated story

    Acknowledgements:

    Discussion

    Chapter 4: Estrogen Receptor Studies at the University of Chicago

    Publisher Summary

    Interaction in vivo

    Effect of steroid structure

    Interaction in vitro

    Binding sites and receptor complexes

    Two-step interaction mechanism

    Isolation of receptor proteins

    Receptors and human breast cancer

    Acknowledgements

    Discussion

    Chapter 5: Studies on Isolation and Characterization of Estrogen Binding Proteins of Calf Uterus

    Summary

    Introduction

    Results

    Nuclear estrogen binding proteins

    Effect of denaturating agents on estrogen binding activity

    Conclusion

    Acknowledgements

    Discussion

    Chapter 6: Studies on the Uterine Cytoplasmic “Estradiol-Receptor”

    Summary

    Introduction

    Methods

    Results

    Discussion

    Acknowledgements

    Discussion

    Chapter 7: Origin and Properties of Target Organ Estradiol Binders

    Discussion

    General Discussion

    II: Androgen Receptors

    Chapter 8: Androgen Receptors: 17β – Hydroxy – 5α – Androstan –3–one and the Translocation of a Cytoplasmic Protein to Cell Nuclei in Prostate

    Summary

    The uptake and retention of androgens by male accessory organs

    5α—dihydrotestosterone receptors

    Steroid specificity of β—protein

    Nuclear retention of the cytosol 5α—dihydrotestosterone-receptor Complex II

    Nuclear acceptor for the specific retention of Complex II

    The role of 5α—dihydrotestosterone in the retention of β—protein by prostate nuclei

    Possible biological implications

    Acknowledgement

    Discussion 

    Chapter 9: The Specific Binding of Steroid-Receptor Complexes to DNA: Evidence from Androgen Receptors in Rat Prostate

    Summary

    Introduction

    Material and Methods

    Results

    Conclusion

    Discussion

    Chapter 10: Androgen Receptors in Rat Ventral Prostate

    Summary

    Introduction

    Material and Methods

    Results

    Acknowledgements

    Discussion

    Chapter 11: Androgenic Receptors in Rat and Human Prostate

    Summary

    Introduction

    Rat tissue

    Materials and Methods

    Human prostate

    Discussion

    III: Progesterone Receptors

    Chapter 12: A Specific Oviduct Target-Tissue Receptor For Progesterone: Identification, Characterization, Partial Purification, Inter-Compartmental Transfer Kinetics and Specific Interaction with the Genome

    Publisher Summary

    Introduction

    Progesterone binding to macromolecules following in vivo administration

    Characterization of the macromolecular-progesterone complex formed by oviduct cytosol in vitro

    Partial purification of the cytoplasmic complex

    Evidence that the binding components mediate progesterone action

    Transfer of the steroid-receptor complex from cytoplasm to nucleus

    Receptor interaction with the genome

    Discussion

    Chapter 13: Progesterone Binding in Rat and Guinea Pig Uterus

    Summary

    Introduction

    Progesterone binding in the rat uterus cytosol [5, 6, 7] Acellular experiments

    Tissue incubations

    In vivo experiments

    Progesterone binding in guinea pig uterus cytosol [8] Progesterone Binding Plasma Protein (PBP)

    Cytosol Progesterone receptor

    Discussion

    Acknowledgements

    Discussion

    Chapter 14: Progesterone Binding Proteins in Rabbit Uterus and Human Endometrium

    Summary

    Introduction

    Materials and Methods

    Results

    Discussion

    Acknowledgements

    Discussion

    IV: Aldosterone Receptors

    Chapter 15: Aldosterone Binding Proteins

    Publisher Summary

    I. Radioautographic studies — toad bladder

    II. Cell fractionation studies — rat kidney

    III. Isolation of aldosterone-binding proteins (ABP)

    IV Characterization of aldosterone-binding proteins

    V. Organ distribution of ABP

    Discussion

    Chapter 16: Towards the Isolation of Aldosterone Receptors from the Toad Bladder

    Summary

    Introduction

    Methods

    Results and discussion

    Extraction of bound aldosterone from the nuclei

    The effect of cortisone on the stimulation of sodium transport by aldosterone

    The effect of cortexolone on sodium transport and on the stimulation of sodium transport by aldosterone

    Acknowledgements

    Discussion

    V: Corticosteroid Receptors

    Chapter 17: Glucocorticoid Receptors in Rat Thymus Cells

    Summary

    Early metabolic effects of glucocorticoids on rat thymus cells in vitro

    Equilibrium and kinetic studies of binding of steroids to intact thymus cells: specific and nonspecific binding [14–16]

    Quantitative estimation of specifically-bound cortisol

    Saturation of specific binding over the physiological range of cortisol concentrations [14–16]

    Competition of other steroids with cortisol for specific binding [14–16]

    Cortexolone as “antiglucocorticoid” in vitro[11, 16]

    ATP requirement for specific binding ? [15,16]

    Rate of association of cortisol to thymus cells at 37 °C and 22 °C [15,16]

    Localization of specifically bound cortisol in the nucleus after incubation at 37 °C [20–22]

    “Cytoplasmic” localization of specifically-bound cortisol after incubation at 3 °C 1)

    Transformation of cytoplasmic to nuclear cortisol-receptor complex2)

    Saturation and specificity of cytoplasmic binding in intact cells 1)

    Extraction of nuclear and cytoplasmic cortisol-receptor complexes: rates of dissociation from the isolated complexes1)

    Comparison of rates of association at 37 °C of cortisol with nuclei and nuclear receptors in intact cells 1)

    Direct binding of steroids to isolated nuclear and cytoplasmic receptors: specificity and saturation1)

    Receptors and hormone sensitivity1)

    Conclusions

    Acknowledgements

    Discussion

    Chapter 18: Glucocorticoid Hormone Receptors

    Summary

    Introduction

    Inducer steroid association with hepatoma tissue culture cells

    Intracellular localization of specifically bound radioactivity

    Localization of the specific receptor

    Binding activity of cell-free extracts

    Kinetics of specific cell-free binding and dissociation

    Effects of other steroids on the specific binding of dexamethasone and cortisol

    Properties of the specific receptor examined in cytoplasmic extracts

    Nature of the specifically bound radioactivity

    Relationship of the specific receptor to other glucocorticoid binding proteins

    Glucocorticoid receptor in other cells

    Other relationships of the specific receptor to hormonal responsiveness

    Acknowledgement

    Discussion

    Chapter 19: Binding of 3H-Cortisol to Macromolecular Components of Rat Liver Cells and its Relation to the Mechanism of Action of Corticosteroids

    Publisher Summary

    Introduction

    Binding of cortisol to the soluble macromolecules of the cytoplasm

    Role of the cytosol binding proteins

    Binding of cortisol to the cell nucleus

    Conclusions

    Acknowledgements

    Discussion

    VI: The Future of Steroid Hormone Receptors

    Chapter 20: The Future of Steroid Hormone Receptor

    Publisher Summary

    Lecturers

    Participants

    Name Index

Product details

  • No. of pages: 424
  • Language: English
  • Copyright: © Pergamon 1972
  • Published: January 1, 1971
  • Imprint: Pergamon
  • eBook ISBN: 9781483160023

About the Editor

Gerhard Raspé

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