Schering Workshop on Steroid Hormone 'Receptors', Berlin, December 7 to 9, 1970

Opening Address

Introduction

I: Estrogen Receptors

Chapter 1: The Regulation of Uterine Concentration of Estrogen Binding Protein

Summary

Introduction

Ontogeny of the estrogen binding protein Period 1: The development of uterine binding

Period 2: Equilibrium

Period 3: Depletion-replenishment cycle after estrogen

Final Remarks

Acknowledgment

Discussion

Chapter 2: Studies on Estradiol-binding in Mammalian Tissues

Summary

Introduction

Discussion

Chapter 3: Estradiol Receptors in the Uterus

Summary

Introduction

Techniques

Early findings: in vivo and tissue in vitro

Cytosol binding proteins Intrinsic vs possible extrinsic specificity

Conformation

Binding parameters

Nuclear receptors “Neo-nuclear” receptor

Non-Histone Chromatin estradiol protein

Receptor and RNA Synthesis

The least complicated story

Acknowledgements:

Discussion

Chapter 4: Estrogen Receptor Studies at the University of Chicago

Publisher Summary

Interaction in vivo

Effect of steroid structure

Interaction in vitro

Binding sites and receptor complexes

Two-step interaction mechanism

Isolation of receptor proteins

Receptors and human breast cancer

Acknowledgements

Discussion

Chapter 5: Studies on Isolation and Characterization of Estrogen Binding Proteins of Calf Uterus

Summary

Introduction

Results

Nuclear estrogen binding proteins

Effect of denaturating agents on estrogen binding activity

Conclusion

Acknowledgements

Discussion

Chapter 6: Studies on the Uterine Cytoplasmic “Estradiol-Receptor”

Summary

Introduction

Methods

Results

Discussion

Acknowledgements

Discussion

Chapter 7: Origin and Properties of Target Organ Estradiol Binders

Discussion

General Discussion

II: Androgen Receptors

Chapter 8: Androgen Receptors: 17β – Hydroxy – 5α – Androstan –3–one and the Translocation of a Cytoplasmic Protein to Cell Nuclei in Prostate

Summary

The uptake and retention of androgens by male accessory organs

5α—dihydrotestosterone receptors

Steroid specificity of β—protein

Nuclear retention of the cytosol 5α—dihydrotestosterone-receptor Complex II

Nuclear acceptor for the specific retention of Complex II

The role of 5α—dihydrotestosterone in the retention of β—protein by prostate nuclei

Possible biological implications

Acknowledgement

Discussion 

Chapter 9: The Specific Binding of Steroid-Receptor Complexes to DNA: Evidence from Androgen Receptors in Rat Prostate

Summary

Introduction

Material and Methods

Results

Conclusion

Discussion

Chapter 10: Androgen Receptors in Rat Ventral Prostate

Summary

Introduction

Material and Methods

Results

Acknowledgements

Discussion

Chapter 11: Androgenic Receptors in Rat and Human Prostate

Summary

Introduction

Rat tissue

Materials and Methods

Human prostate

Discussion

III: Progesterone Receptors

Chapter 12: A Specific Oviduct Target-Tissue Receptor For Progesterone: Identification, Characterization, Partial Purification, Inter-Compartmental Transfer Kinetics and Specific Interaction with the Genome

Publisher Summary

Introduction

Progesterone binding to macromolecules following in vivo administration

Characterization of the macromolecular-progesterone complex formed by oviduct cytosol in vitro

Partial purification of the cytoplasmic complex

Evidence that the binding components mediate progesterone action

Transfer of the steroid-receptor complex from cytoplasm to nucleus

Receptor interaction with the genome

Discussion

Chapter 13: Progesterone Binding in Rat and Guinea Pig Uterus

Summary

Introduction

Progesterone binding in the rat uterus cytosol [5, 6, 7] Acellular experiments

Tissue incubations

In vivo experiments

Progesterone binding in guinea pig uterus cytosol [8] Progesterone Binding Plasma Protein (PBP)

Cytosol Progesterone receptor

Discussion

Acknowledgements

Discussion

Chapter 14: Progesterone Binding Proteins in Rabbit Uterus and Human Endometrium

Summary

Introduction

Materials and Methods

Results

Discussion

Acknowledgements

Discussion

IV: Aldosterone Receptors

Chapter 15: Aldosterone Binding Proteins

Publisher Summary

I. Radioautographic studies — toad bladder

II. Cell fractionation studies — rat kidney

III. Isolation of aldosterone-binding proteins (ABP)

IV Characterization of aldosterone-binding proteins

V. Organ distribution of ABP

Discussion

Chapter 16: Towards the Isolation of Aldosterone Receptors from the Toad Bladder

Summary

Introduction

Methods

Results and discussion

Extraction of bound aldosterone from the nuclei

The effect of cortisone on the stimulation of sodium transport by aldosterone

The effect of cortexolone on sodium transport and on the stimulation of sodium transport by aldosterone

Acknowledgements

Discussion

V: Corticosteroid Receptors

Chapter 17: Glucocorticoid Receptors in Rat Thymus Cells

Summary

Early metabolic effects of glucocorticoids on rat thymus cells in vitro

Equilibrium and kinetic studies of binding of steroids to intact thymus cells: specific and nonspecific binding [14–16]

Quantitative estimation of specifically-bound cortisol

Saturation of specific binding over the physiological range of cortisol concentrations [14–16]

Competition of other steroids with cortisol for specific binding [14–16]

Cortexolone as “antiglucocorticoid” in vitro[11, 16]

ATP requirement for specific binding ? [15,16]

Rate of association of cortisol to thymus cells at 37 °C and 22 °C [15,16]

Localization of specifically bound cortisol in the nucleus after incubation at 37 °C [20–22]

“Cytoplasmic” localization of specifically-bound cortisol after incubation at 3 °C 1)

Transformation of cytoplasmic to nuclear cortisol-receptor complex2)

Saturation and specificity of cytoplasmic binding in intact cells 1)

Extraction of nuclear and cytoplasmic cortisol-receptor complexes: rates of dissociation from the isolated complexes1)

Comparison of rates of association at 37 °C of cortisol with nuclei and nuclear receptors in intact cells 1)

Direct binding of steroids to isolated nuclear and cytoplasmic receptors: specificity and saturation1)

Receptors and hormone sensitivity1)

Conclusions

Acknowledgements

Discussion

Chapter 18: Glucocorticoid Hormone Receptors

Summary

Introduction

Inducer steroid association with hepatoma tissue culture cells

Intracellular localization of specifically bound radioactivity

Localization of the specific receptor

Binding activity of cell-free extracts

Kinetics of specific cell-free binding and dissociation

Effects of other steroids on the specific binding of dexamethasone and cortisol

Properties of the specific receptor examined in cytoplasmic extracts

Nature of the specifically bound radioactivity

Relationship of the specific receptor to other glucocorticoid binding proteins

Glucocorticoid receptor in other cells

Other relationships of the specific receptor to hormonal responsiveness

Acknowledgement

Discussion

Chapter 19: Binding of 3H-Cortisol to Macromolecular Components of Rat Liver Cells and its Relation to the Mechanism of Action of Corticosteroids

Publisher Summary

Introduction

Binding of cortisol to the soluble macromolecules of the cytoplasm

Role of the cytosol binding proteins

Binding of cortisol to the cell nucleus

Conclusions

Acknowledgements

Discussion

VI: The Future of Steroid Hormone Receptors

Chapter 20: The Future of Steroid Hormone Receptor

Publisher Summary

Lecturers

Participants

Name Index