Scale-Up and Automation in Plant Propagation

Scale-Up and Automation in Plant Propagation

Cell Culture and somatic cell Genetics of Plants

1st Edition - June 28, 1991

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  • Editor: Indra Vasil
  • eBook ISBN: 9780323157384

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Scale-Up and Automation in Plant Propagation reviews methods of automation and scale-up of plant propagation in vitro. It looks at the large scale clonal propagation of plants, or micropropagation, as the first major practical application of plant biotechnology. It also discusses the advantages and limitations of micropropagation and evaluates current methods of commercial micropropagation. Organized into 13 chapters, this volume begins with an overview of the benefits of scaling up and automating plant propagation before proceeding with a discussion of synthetic seeds and their use for plant propagation, along with problems and economic considerations associated with synthetic seed technology. It then considers the implementation of somatic embryogenesis technology for clonal forestry, the development and commercialization of bioreactor technology for automated propagation of potato microtubers and lily microbulbs, and approaches to automated propagation of fruit trees. Other chapters focus on issues of cost reduction and development of ""new"" products, scale-up and operation of prototype bioreactors for plant propagation, and application of machine vision technology to scale-up and automated evaluation of somatic embryogenesis in sweet potato. The book also describes methods of measurement and control of the environment in culture, environmental factors affecting photosynthesis, and use of robotics and field transplanters in the automation of plant propagation. Scientists and plant breeders will find this book extremely useful.

Table of Contents

  • Contributors

    General Preface


    Contents of Previous Volumes

    1 Rationale for the Scale-Up and Automation of Plant Propagation



    2 Current Methods of Commercial Micropropagation

    I. Introduction

    II. Current Production Systems

    III. Production Cost Components

    IV. Development of Cost-Effective Production Systems

    V. Conclusions


    3 Synthetic Seed Technology

    I. Introduction

    II. Uncoated, Desiccated Synthetic Seeds

    III. Coated, Desiccated Synthetic Seeds

    IV. Coated, Hydrated Synthetic Seeds

    V. Uncoated, Hydrated Synthetic Seeds (Fluid Drilling)

    VI. Maturation of Somatic Embryos

    VII. Problems with Synthetic Seeds

    VIII. Applications

    IX. Mechanization

    X. Economic Considerations

    XI. Conclusions


    4 Development of an Embryogénie System for Automated Propagation of Forest Trees

    I. Introduction

    II. Development of an Embryogénie System

    III. Plantlet Regeneration and Establishment

    IV. Cryopreservation

    V. Culture Scale-Up

    VI. Delivery Systems

    VII. Singulation and Selection of Embryos

    VIII. Field Testing and Implementation

    IX. Genetic Diversity


    5 Automated Propagation of Microtubers of Potato

    I. Introduction

    II. Factors To Be Considered in Microtuber Bioreactor Design

    III. A Novel Microtuber Bioreactor

    IV. Conclusions


    6 Automated Propagation of Microbulbs of Lilies

    I. Introduction

    II. Regulation of in Vitro Differentiation and Growth in Lilium for Large-Scale Propagation

    III. Automated Propagation of Microbulbs from Bulbscales Using Bioreactor Techniques

    IV. Micropropagation from Bulbscales of Microbulbs in Nonsterile Conditions

    V. Micropropagation from Suspension-Cultured Cells

    VI. Robotics in Lilium Micropropagation

    VII. Automated Process for Transplantation of Lilium Microbulbs into Soil

    VIII. Idealized Automated Micropropagation Processes for Lilium in the Future-Problems and Perspective


    7 Problems and Perspectives for the Automated Propagation of Fruit Trees

    I. Introduction

    II. Technological Aspects Related to Automation

    III. Approaches to Automation of Fruit Tree Micropropagation

    IV. Conclusions and Perspectives


    8 Bioreactors and Image Analysis for Scale-Up and Plant Propagation

    I. Introduction

    II. Image Analysis

    III. Refining the Imaging Software

    IV. Embryo Concentration Assessment

    V. Bioreactor Operation

    VI. Conclusion


    9 Automated Evaluation of Somatic Embryogenesis in Sweet Potato by Machine Vision

    I. Introduction

    II. Materials and Methods

    III. System Description and Operation

    IV. Image Analysis

    V. The Measurement Process

    VI. Verification of the Inspection System

    VII. On-Line Analysis

    VIII. Conclusions


    10 Control of Contamination in Automated Plant Propagation

    I. Introduction

    II. Origin and Nature of Contamination in Micropropagation

    III. Identification of Contaminants

    IV. Control of Contamination

    V. Monitoring and Control of Contamination during Automated Production

    VI. Conclusion


    11 Controlled Environments in Conventional and Automated Micropropagation

    I. Introduction

    II. Environmental Factors in Culture

    III. Measurement and Control of the Environment

    IV. Environmental Effect on Photosynthetic Growth and Development

    V. Advantages of Photoautotrophic Micropropagation

    VI. Concluding Remarks


    12 The Use of Robotics in Automated Plant Propagation

    I. Introduction

    II. Plantlet Production Through Tissue Culture

    III. Necessity of Robots and Required Functions

    IV. Development of the Prototype Robot System

    V. Second Generation Robot

    VI. Conclusions


    13 The Use of Automated Field Transplanters in Plant Propagation

    I. Introduction

    II. Review

    III. Analysis

    IV. Projections

    V. Conclusions



Product details

  • No. of pages: 292
  • Language: English
  • Copyright: © Academic Press 1991
  • Published: June 28, 1991
  • Imprint: Academic Press
  • eBook ISBN: 9780323157384

About the Editor

Indra Vasil

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