Robotic Cell Manipulation

Robotic Cell Manipulation

1st Edition - May 31, 2022

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  • Author: Dong Sun
  • eBook ISBN: 9780323852609
  • Paperback ISBN: 9780323852593

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Robotic Cell Manipulation introduces up-to-date research to realize this new theme of medical robotics. The book is organized in three levels: operation tools (e.g., optical tweezers, microneedles, dielectrophoresis, electromagnetic devices, and microfluidic chips), manipulation types (e.g., microinjection, transportation, rotation fusion, adhesion, separation, etc.), and potential medical applications (e.g., micro-surgery, biopsy, gene editing, cancer treatment, cell-cell interactions, etc.). The technology involves different fields such as robotics, automation, imaging, microfluidics, mechanics, materials, biology and medical sciences. The book provides systematic knowledge on the subject, covering a wide range of basic concepts, theories, methodology, experiments, case studies and potential medical applications. It will enable readers to promptly conduct a systematic review of research and become an essential reference for many new and experienced researchers entering this unique field.

Key Features

  • Introduces the applications of robot-assisted manipulation tools in various cell manipulation tasks
  • Defines many essential concepts in association with the robotic cell manipulation field, including manipulation strategy and manipulation types
  • Introduces basic concepts and knowledge on various manipulation devices and tasks
  • Describes some cutting-edge cell manipulation technologies and case studies


Graduate and professional level in the fields of biomedical engineering, robotics, advanced manufacturing, biology, and medicine. Job titles of prospective readers include: PhD student, professors, medical doctors, R&D engineer, consultants, technician, and government officials. Undergraduate level in engineering, biomedical science, surgery etc.

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • Dedication
  • Preface
  • Acknowledgments
  • 1. Introduction
  • 1.1. Overview of robot-facilitated cell manipulation
  • 1.2. Outline of the book
  • 1.3. Conclusions
  • 2. Cell manipulation tools
  • 2.1. Introduction
  • 2.2. Microneedle
  • 2.3. Optical tweezers
  • 2.4. Electrokinetics
  • 2.5. Magnetic manipulator
  • 2.6. Atomic force microscopy
  • 2.7. Imaging for intracellular manipulation
  • 2.8. Conclusions
  • 3. Robotic cell injection
  • 3.1. Introduction
  • 3.2. Robot-assisted cell microinjection system with microneedles
  • 3.3. Hybrid position and force control for automated batch injection of cells
  • 3.4. Universal piezo-driven ultrasonic cell microinjection
  • 3.5. Automated microinjection for small human cells
  • 3.6. Automated high-productivity microinjection for adherent cells
  • 3.7. Single-cell transfection through precise microinjection with quantitatively controlled injection volumes
  • 3.8. Characterization of mechanical properties of cells through microinjection
  • 3.9. Conclusions
  • 4. Cell stretching and compression
  • 4.1. Introduction
  • 4.2. Cell stretching with optical tweezers
  • 4.3. Probing cell biophysical behavior based on actin cytoskeleton modeling of cells and stretching manipulation with optical tweezers
  • 4.4. Cell stretching with dielectrophoresis technology
  • 4.5. Cell compression under mechanical confinement
  • 4.6. Magnet-based cell deformation for intracellular delivery
  • 4.7. Conclusions
  • 5. Cell transport with optical tweezers
  • 5.1. Introduction
  • 5.2. Basic theory and methods
  • 5.3. Motion and path planning for automatic cell transportation
  • 5.4. Unified motion control design
  • 5.5. Multiple cell transportation for cell pairing
  • 5.6. Cell transportation for multiprocessing automation tasks
  • 5.7. Conclusions
  • 6. Cell rotation
  • 6.1. Introduction
  • 6.2. Automated in-plane rotation of cells using a robot tweezers manipulation system
  • 6.3. Automated out-of-plane rotation of cells using a robot tweezers manipulation system
  • 6.4. Conclusions
  • 7. Three-dimensional image reconstruction and intracellular surgery
  • 7.1. Introduction
  • 7.2. 3D image reconstruction
  • 7.3. Robot-assisted intracellular delivery with 3D image reconstruction information
  • 7.4. Conclusions
  • 8. Cell sorting and separation
  • 8.1. Introduction
  • 8.2. Cell sorting using combined optical tweezers and microfluidic chip technology
  • 8.3. Cell isolation and deposition
  • 8.4. A simplified sheathless cell separation approach
  • 8.5. Conclusions
  • 9. Cell stimulation and migration control
  • 9.1. Introduction
  • 9.2. Dynamic model of chemoattractant-induced cell migration
  • 9.3. Cell migration control using a stimulus-induced robotic manipulation system
  • 9.4. Electrical stimulation based on calcium spike patterns of MSCs to improve osteogenic differentiation
  • 9.5. Conclusions
  • 10. Cell patterning
  • 10.1. Introduction
  • 10.2. Cell patterning with robotically controlled optical tweezers
  • 10.3. Cell patterning using a dielectrophoresis-based multilayer scaffold structure
  • 10.4. Cell patterning using a gravitational sedimentation-based microfluidic approach
  • 10.5. Conclusions
  • 11. Cell adhesion
  • 11.1. Introduction
  • 11.2. Manipulating cell adhesion with optical tweezers
  • 11.3. Adhesion-mediated cell–cell interaction
  • 11.4. A case study of cell adhesion characterization
  • 11.5. Conclusions
  • 12. Cell fusion
  • 12.1. Introduction
  • 12.2. Laser-induced fusion with optical tweezers
  • 12.3. Cell fusion with combined optical tweezers and microwell array technology
  • 12.4. A case study of transforming liver cancer cells into tumor initiating-like cells by cell fusion
  • 12.5. Conclusions
  • 13. Cell navigation and delivery in vivo
  • 13.1. Introduction
  • 13.2. In vivo navigation of single cells with an optical tweezers-based manipulator
  • 13.3. Magnetic microrobot for carrying and delivering cells in vivo
  • 13.4. Precise delivery of stem cells for cancer therapy using magnet-driven and image-guided degradable microrobots
  • 13.5. Conclusions
  • 14. Organelle biopsy and gene editing of single cells
  • 14.1. Introduction
  • 14.2. Automated organelle biopsy of single cells using microneedles
  • 14.3. Gene-delivery approaches for MSC function improvement
  • 14.4. Automated optical tweezers manipulation for mitochondrial transfer
  • 14.5. Conclusions
  • 15. Summary
  • 15.1. Operation tools for cell manipulations
  • 15.2. Cell manipulation types
  • 15.3. Potential medical applications
  • Index

Product details

  • No. of pages: 546
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: May 31, 2022
  • Imprint: Academic Press
  • eBook ISBN: 9780323852609
  • Paperback ISBN: 9780323852593

About the Author

Dong Sun

Dong Sun is currently a Chair Professor and Head of the Department of Biomedical Engineering, and also Director of the Centre of Robotics and Automation, City University of Hong Kong. He is among the leading contributors worldwide in pioneering work in robotic manipulation of biological cells. His research has breakthrough in the use of combined robotics and various micro-engineering tools including optical tweezers, micro-needles and electromagnetic devices to achieve cell manipulation, diagnosis and micro-surgery at the single cell level. He led the invention of the magnetically driven microrobots that deliver cells to precise locations in the body. Over the past 20 years, he has co-authored 17 books and book chapters, 420 journal and conference papers with h-Index of over 50, and holds 13 international patents. He has received a lot of awards including best paper awards, Natural Science Award from China, and Hong Kong Awards for Industry. He is a fellow of Canadian Academy of Engineering and a fellow of IEEE.

Affiliations and Expertise

Chair Professor and Head of the Department of Biomedical Engineering, Director of the Centre of Robotics and Automation, City University of Hong Kong

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