Robotic Cell Manipulation

Chapter 1 Introduction
1.1 Cell Characteristics and Manipulation
1.2 Robot-assisted Cell Manipulation
1.3 About This Book: Chapter Introduction and Arrangement

Chapter 2 Manipulation Tools for Cells
2.1  Microneedles
2.2  Optical Tweezers
2.3   Dielectrophoresis
2.4  Magnetic Devices
2.5  Microfluidics
2.6  Summary

Chapter 3 Robotic Cell Injection
3.1  Introduction
3.2  Robot-assisted Cell Microinjection System with Microneedles
3.3  Automated Batch Injection of Cells With Position and Force Control
3.4  Universal Piezo-driven Ultrasonic Cell Microinjection
3.5  Characterizing Mechanical Properties of Cells Through Microinjection
3.6  High-throughput Automated Microinjection for Human Cells With Small Size
3.7  Single Cell Transfection Through Precise Microinjection With Quantitatively Controlled Injection Volumes
3.8  Automated High-Productivity Microinjection for Adherent Cells
3.9  Summary

Chapter 4 Cell Stretching and Compression
4.1  Introduction
4.2  Cell Stretching With Optical Tweezers
4.3  Probing Cell Biophysical Behavior based on Actin Cytoskeleton Modeling of Cells
and Stretching Manipulation With Optical Tweezers
4.4  Cell Stretching With Dielectrophoresis Technology
4.5  Cell Compression under Mechanical Confinement
4.6  Magnetic-based Cell Deformation for Intracellular Delivery
4.7  Summary

Chapter 5 Cell Transportation
5.1  Introduction
5.2  Model of Robotic Cell Transportation With Optical Tweezers
5.3  Motion Planning for Transporting Cells
5.4  Automated Single Cell Transportation Control
5.5  Automated Multi-Cell Transportation Control
5.6  Automated Transportation of Cells in Multistep Cell Surgery
5.7   Summary

Chapter 6 Cell Rotation
6.1  Introduction
6.2  Model of Cell Rotation With Robotically Controlled Optical Tweezers
6.3  Cell Out-of-Plane Rotation Control
6.4  Translational and Rotational Manipulation of Cells by Using Optically Actuated Microrobots
6.5  Summary

Chapter 7 3D Image Reconstruction for Intracellular Surgery
7.1  Introduction
7.2  3D Reconstruction Methods for Cells
7.3  Experiments
7.4  Intracellular Delivery Strategy
7.5  Intracellular Surgery With Cellular 3D Image Reconstruction
7.6  Summary

Chapter 8 Cell Sorting and Separation
8.1  Introduction
8.2  Cell Sorting With Combined Optical Tweezers and Microfluidic Chip
8.3  Cell Isolation and Deposition with Combined Microwell Array and Optical Tweezers
8.3  Cell Separation Using Combined Gravitational-Sedimentationbased Prefocusing and Dielectrophoretic Separation
8.4  A Microfluidic Device for Isolation and Characterization of Transendothelial Migrating Cancer Cells
8.5  Summary

Chapter 9 Cell Stimulation and Migration
9.1  Introduction
9.2  Model of Chemoattractant-Induced Cell Migration
9.3  Measurement of Cell Protrusion Force in Chemoattractant-Induced Cell Migration
9.4  Cell Migration Control in Chemoattractant-Induced Cell Migration
9.5  Cell Migration Control Induced by a Chemoattractant-Loaded Microbead
9.6  Microfluidic Single-Cell Array Platform for Clonal Expansion Under Chemical/Electrical Stimuli
9.7  Electrical Stimulation for Stem Cell Differentiation
9.8 Summary

Chapter 10 Cell Patterning
10.1  Introduction
10.2  Cell Patterning With Robotically Controlled Optical Tweezers
10.3  Cell Patterning Using Gravitational Sedimentation-based Approach on Microfluidic Device
10.4  Cell Patterning Using Dielectrophoresis via Multi-layer Scaffold Structure
10.5  Dielectrophoresis-based Cell Patterning for Tissue Engineering
10.6  Summary

Chapter 11 Cell Adhesion and Cell-to-Cell Interaction
11.1  Introduction
11.2  Cell Adhesion Manipulation
11.3   Cell-to-Cell Interaction Through Cell Adhesion
11.4  Cell Adhesion Manipulation to Probe Cell Migration Mechanism During Cell-to-Cell Interaction
11.5  Summary

Chapter 12 Cell Fusion
12.1  Introduction
12.2  A Basic Cell Fusion Approach with Optical Tweezers
12. 3  Laser-induced Cell Fusion Based on Microwell Array Technology
12.4  Automated Pairing Manipulation of Cells With a Robot-Tweezers Manipulation System
12.5  Fusion between Cancer Cell and Stem Cell for Generating Tumor Initiating-like Cells
12.6  Increasing Physical Size and Nucleation Status of Human Pluripotent
Stem Cell-derived Ventricular Cardiomyocytes by Cell Fusion
12.7  Summary

Chapter 13 Cell Navigation and Microrobot Delivery In Vivo
13.1  Introduction
13.2  In Vivo Single Cell Transportation with Optical Tweezers Manipulator
13.3  Collision-avoidance Control for In Vivo Transportation of Cells
13.4  Simultaneous Localization and Mapping-Based In Vivo Navigation Control of Microparticles
13.5   A Magnetic Microrobot for Carrying and Delivering Cells In Vivo
13.6  Magnetic Degradable Microrobots for the Precise Delivery of Engineered Stem Cells for Cancer Therapy
13.7  Summary

Chapter 14 Organelle Biopsy on Single Cells
14.1  Introduction
14.2  Microneedle-based Single Cell Biopsy System and Process
14.3  Organelle Extraction and Biopsy
14.4  Mitochondria Transplantation
14.5  Summary

Chapter 15 Single Cell Gene Editing
15.1  Introduction
15.2  A Gene Editing Tool by Combining Robotic Microinjection and Crispr/Cas9 System
15.3  Gene Knock-in
15.4  Gene Knock-out
15.5 Summary