Robotic Cell Manipulation

Robotic Cell Manipulation

1st Edition - June 1, 2022

Write a review

  • Author: Dong Sun
  • Paperback ISBN: 9780323852593

Purchase options

Purchase options
Available for Pre-Order
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Robotic Cell Manipulation introduces up-to-date research to realize this new theme of medical robotics. The book is organized in three levels: operation tools (e.g., optical tweezers, microneedles, dielectrophoresis, electromagnetic devices, and microfluidic chips), manipulation types (e.g., microinjection, transportation, rotation fusion, adhesion, separation, etc.), and potential medical applications (e.g., micro-surgery, biopsy, gene editing, cancer treatment, cell-cell interactions, etc.). The technology involves different fields such as robotics, automation, imaging, microfluidics, mechanics, materials, biology and medical sciences. The book provides systematic knowledge on the subject, covering a wide range of basic concepts, theories, methodology, experiments, case studies and potential medical applications. It will enable readers to promptly conduct a systematic review of research and become an essential reference for many new and experienced researchers entering this unique field.

Key Features

  • Introduces the applications of robot-assisted manipulation tools in various cell manipulation tasks
  • Defines many essential concepts in association with the robotic cell manipulation field, including manipulation strategy and manipulation types
  • Introduces basic concepts and knowledge on various manipulation devices and tasks
  • Describes some cutting-edge cell manipulation technologies and case studies


Graduate and professional level in the fields of biomedical engineering, robotics, advanced manufacturing, biology, and medicine. Job titles of prospective readers include: PhD student, professors, medical doctors, R&D engineer, consultants, technician, and government officials. Undergraduate level in engineering, biomedical science, surgery etc.

Table of Contents

  • Chapter 1 Introduction
    1.1 Cell Characteristics and Manipulation
    1.2 Robot-assisted Cell Manipulation
    1.3 About This Book: Chapter Introduction and Arrangement

    Chapter 2 Manipulation Tools for Cells
    2.1  Microneedles
    2.2  Optical Tweezers
    2.3   Dielectrophoresis
    2.4  Magnetic Devices
    2.5  Microfluidics
    2.6  Summary

    Chapter 3 Robotic Cell Injection
    3.1  Introduction
    3.2  Robot-assisted Cell Microinjection System with Microneedles
    3.3  Automated Batch Injection of Cells With Position and Force Control
    3.4  Universal Piezo-driven Ultrasonic Cell Microinjection
    3.5  Characterizing Mechanical Properties of Cells Through Microinjection
    3.6  High-throughput Automated Microinjection for Human Cells With Small Size
    3.7  Single Cell Transfection Through Precise Microinjection With Quantitatively Controlled Injection Volumes
    3.8  Automated High-Productivity Microinjection for Adherent Cells
    3.9  Summary

    Chapter 4 Cell Stretching and Compression
    4.1  Introduction
    4.2  Cell Stretching With Optical Tweezers
    4.3  Probing Cell Biophysical Behavior based on Actin Cytoskeleton Modeling of Cells
    and Stretching Manipulation With Optical Tweezers
    4.4  Cell Stretching With Dielectrophoresis Technology
    4.5  Cell Compression under Mechanical Confinement
    4.6  Magnetic-based Cell Deformation for Intracellular Delivery
    4.7  Summary

    Chapter 5 Cell Transportation
    5.1  Introduction
    5.2  Model of Robotic Cell Transportation With Optical Tweezers
    5.3  Motion Planning for Transporting Cells
    5.4  Automated Single Cell Transportation Control
    5.5  Automated Multi-Cell Transportation Control
    5.6  Automated Transportation of Cells in Multistep Cell Surgery
    5.7   Summary

    Chapter 6 Cell Rotation
    6.1  Introduction
    6.2  Model of Cell Rotation With Robotically Controlled Optical Tweezers
    6.3  Cell Out-of-Plane Rotation Control
    6.4  Translational and Rotational Manipulation of Cells by Using Optically Actuated Microrobots
    6.5  Summary

    Chapter 7 3D Image Reconstruction for Intracellular Surgery
    7.1  Introduction
    7.2  3D Reconstruction Methods for Cells
    7.3  Experiments
    7.4  Intracellular Delivery Strategy
    7.5  Intracellular Surgery With Cellular 3D Image Reconstruction
    7.6  Summary

    Chapter 8 Cell Sorting and Separation
    8.1  Introduction
    8.2  Cell Sorting With Combined Optical Tweezers and Microfluidic Chip
    8.3  Cell Isolation and Deposition with Combined Microwell Array and Optical Tweezers
    8.3  Cell Separation Using Combined Gravitational-Sedimentationbased Prefocusing and Dielectrophoretic Separation
    8.4  A Microfluidic Device for Isolation and Characterization of Transendothelial Migrating Cancer Cells
    8.5  Summary

    Chapter 9 Cell Stimulation and Migration
    9.1  Introduction
    9.2  Model of Chemoattractant-Induced Cell Migration
    9.3  Measurement of Cell Protrusion Force in Chemoattractant-Induced Cell Migration
    9.4  Cell Migration Control in Chemoattractant-Induced Cell Migration
    9.5  Cell Migration Control Induced by a Chemoattractant-Loaded Microbead
    9.6  Microfluidic Single-Cell Array Platform for Clonal Expansion Under Chemical/Electrical Stimuli
    9.7  Electrical Stimulation for Stem Cell Differentiation
    9.8 Summary

    Chapter 10 Cell Patterning
    10.1  Introduction
    10.2  Cell Patterning With Robotically Controlled Optical Tweezers
    10.3  Cell Patterning Using Gravitational Sedimentation-based Approach on Microfluidic Device
    10.4  Cell Patterning Using Dielectrophoresis via Multi-layer Scaffold Structure
    10.5  Dielectrophoresis-based Cell Patterning for Tissue Engineering
    10.6  Summary

    Chapter 11 Cell Adhesion and Cell-to-Cell Interaction
    11.1  Introduction
    11.2  Cell Adhesion Manipulation
    11.3   Cell-to-Cell Interaction Through Cell Adhesion
    11.4  Cell Adhesion Manipulation to Probe Cell Migration Mechanism During Cell-to-Cell Interaction
    11.5  Summary

    Chapter 12 Cell Fusion
    12.1  Introduction
    12.2  A Basic Cell Fusion Approach with Optical Tweezers
    12. 3  Laser-induced Cell Fusion Based on Microwell Array Technology
    12.4  Automated Pairing Manipulation of Cells With a Robot-Tweezers Manipulation System
    12.5  Fusion between Cancer Cell and Stem Cell for Generating Tumor Initiating-like Cells
    12.6  Increasing Physical Size and Nucleation Status of Human Pluripotent
    Stem Cell-derived Ventricular Cardiomyocytes by Cell Fusion
    12.7  Summary

    Chapter 13 Cell Navigation and Microrobot Delivery In Vivo
    13.1  Introduction
    13.2  In Vivo Single Cell Transportation with Optical Tweezers Manipulator
    13.3  Collision-avoidance Control for In Vivo Transportation of Cells
    13.4  Simultaneous Localization and Mapping-Based In Vivo Navigation Control of Microparticles
    13.5   A Magnetic Microrobot for Carrying and Delivering Cells In Vivo
    13.6  Magnetic Degradable Microrobots for the Precise Delivery of Engineered Stem Cells for Cancer Therapy
    13.7  Summary

    Chapter 14 Organelle Biopsy on Single Cells
    14.1  Introduction
    14.2  Microneedle-based Single Cell Biopsy System and Process
    14.3  Organelle Extraction and Biopsy
    14.4  Mitochondria Transplantation
    14.5  Summary

    Chapter 15 Single Cell Gene Editing
    15.1  Introduction
    15.2  A Gene Editing Tool by Combining Robotic Microinjection and Crispr/Cas9 System
    15.3  Gene Knock-in
    15.4  Gene Knock-out
    15.5 Summary

Product details

  • No. of pages: 520
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: June 1, 2022
  • Imprint: Academic Press
  • Paperback ISBN: 9780323852593

About the Author

Dong Sun

Dong Sun is currently a Chair Professor and Head of the Department of Biomedical Engineering, and also Director of the Centre of Robotics and Automation, City University of Hong Kong. He is among the leading contributors worldwide in pioneering work in robotic manipulation of biological cells. His research has breakthrough in the use of combined robotics and various micro-engineering tools including optical tweezers, micro-needles and electromagnetic devices to achieve cell manipulation, diagnosis and micro-surgery at the single cell level. He led the invention of the magnetically driven microrobots that deliver cells to precise locations in the body. Over the past 20 years, he has co-authored 17 books and book chapters, 420 journal and conference papers with h-Index of over 50, and holds 13 international patents. He has received a lot of awards including best paper awards, Natural Science Award from China, and Hong Kong Awards for Industry. He is a fellow of Canadian Academy of Engineering and a fellow of IEEE.

Affiliations and Expertise

Chair Professor and Head of the Department of Biomedical Engineering, Director of the Centre of Robotics and Automation, City University of Hong Kong

Ratings and Reviews

Write a review

There are currently no reviews for "Robotic Cell Manipulation"