This publication summarizes the current status of our understanding of RNA, with particular emphasis on the chemistry of this key biological molecule. The various RNAs covered are messenger RNA, ribosomal RNA, transfer RNA and RNA enzymes (ribozymes). The different chapters detail biophysical and chemical methods to investigate RNA structure and function, the synthesis of native and modified RNAs and the latest advances in our understanding of the vast array of biological processes in which RNA is involved.


For all those interested in the structural biology, enzymology, biochemistry and molecular biology of RNA.

Table of Contents

Paper headings. A spectroscopist's view of RNA conformation: RNA structural motifs (P.B. Moore). Thermodynamics of RNA secondary structure formation (T. Xia, D.H. Mathews and D.H. Turner). RNA structures determined by X-ray crystallography (J.A. Doudna). RNA conformational dynamics (D.M. Crothers). Classical and novel chemical tools for RNA structure probing (R. Giegé et al.). Chemical RNA synthesis (including RNA with unusual constituents) (Y. Komatsu, E. Ohtsuka). The phosphoryl transfer reactions in pre-messenger RNA splicing (M.A. Garcia-Blanco et al.). RNA editing (M. Öhman, B.L. Bass). Ribonuclease P (A. Vioque, S. Altman). Ribozyme selection (A.D. Ellington, M.P. Robertson). Ribozyme enzymology (J.K Strauss-Soukup, S.A. Strobel). Viroids (R.H. Symons). Structural elements of ribosomal RNA (S.T. Gregory et al.). Turnover of mRNA in eukaryotic cells (S. Tharun, R. Parker). Applications of ribonucleotide analogues in RNA biochemistry (S. Verma et al.). RNA in biotechnology: towards a role for ribozymes in gene therapy (M. Warashina et al.). Appendix: Modified nucleosides from RNA (J.A. McCloskey).


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© 2001
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