COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
RNA Polymerase and Associated Factors, Part B - 1st Edition - ISBN: 9780121821753, 9780080883878

RNA Polymerase and Associated Factors, Part B, Volume 274

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: Sankar Adhya
eBook ISBN: 9780080883878
Imprint: Academic Press
Published Date: 14th October 1996
Page Count: 566


This product is currently not available for sale.

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

Basal Transcription and Regulation of Transcription: Transcription Factors, Activators, and Repressors: H.E. Choy, A Complete Ribosome-Free in Vitro Transcription System Responsive to Extract of Escherichiacoli. A. Miller, M. Choi, M.A. Glucksmann-Kuis, X. Dai, and L.B. Rothman-Denes, Single-Stranded DNA-Binding Proteins as Transcriptional Activators. S.E. Hasnain, S. Habib, A. Jain, S. Burma, and B. Mukherjee, Host Factor with Single-Stranded DNA-Binding Activity Involved in Transcription from Baculovirus Polyhedrin Promoter. N. Goosen, P. van Ulsen, L. Zulianello, and P. van de Putte, Transcription Activiation by Histone-like Protein IHF. D.M. Hinton, R. March-Amegadzie, J.S. Gerber, and M. Sharma, Bacteriophage T4 Middle Transcription System: T4-Modified RNA Polymerase, AsiA (Sigma-70 Binding Protein), and Transcriptional Activator MotA. H. Ge, E. Martinez, C.-M. Chiang, and R.G. Roeder, Activator-Dependent Transcription by Mammalian RNA Polymerase II: In Vitro Reconstitution with General Transcription Factors and Cofactors. E. Maldonado, R. Drapkin, and D. Reinberg, Purification of Human RNA Polymerase II and General Transcription Factors. W.-C. Yeh, J. Hou, and S.L. McKnight, Purification and Characterization of Gene-Specific Transcription Factors from Rat Liver: c/EBP, GABP, and IL-4 Stat. M. Zhong, J. Wisniewski, M. Fritsch, M. Mizuguchi, A. Orosz, P. Jedlicka, and C. Wu, Purification of Heat ShockTranscription Factor of Drosophila. J. Greenblatt and C.J. Ingles, Interaction between Acidic Transcriptional Activation Domains of Activator Protein VP16 Herpes Simplex Virus and TRIID. M. Carey, D.Tantin, T. Chi, R. Hori, and S. Pyo, Biochemical Mechanism of Transcriptional Activation by Ga14-VP16. R. Valcarcel and H.G. Stunnenberg, Retinoid-Dependent in Vitro Transcription. D. Thanos and T. Maniatis, In Vitro Assembly of Enhancer Complexes. G. Stegar, J. Ham, and M. Yaniv, E2 Proteins: Modulators of Papillomavirus Transcription and Replication. Chromatin as Templates for Transcription: L.P. ONeill and B.M. Turner, Immunoprecipitation of Chromatin. F. Thoma, Mapping of Nucleosome Positions. M.P.Kladde and R.T. Simpson, Chromatin Structure Mapping in Vivo Using Methyltransferases. A.E. Sippel, H. Saueressig, M.C. Huber, H.C. Hoefer, A. Stief, U. Borgmeyer, and C. Bonifer, Identification of cis-Acting Elements as DNase I Hypersensitive Sites in Lysozyme Gene Chromatin. V.M. Studitsky, D.J. Clarke, and G. Felsenfeld, Preparation of Nucleosomal Templates for Transcription in Vitro. K. Ura and A.P. Wolffe, Reconstruction of Transcriptionally Active and Silent Chromatin. C. Ueguchi and T. Mizuno, Purification of H-NS Protein and Its Regulatory Effect on Transcription in Vitro. R.T. Utley, T.A.O. Hughes, L.-J. Juan, J. Cite, C.C. Adams, and J.L. Workman, In Vitro Analysis of Transcription Factor Binding to Nucleosomes and Nucleosome Disruption/Displacement. T. Tsukiyama and C. Wu, Purification of GAGA Factor of Drosophila and Its Role in Nucleosome Disruption. M.C. Barton and B.M. Emerson, Regulated Gene Expression in Reconstituted Chromatin and Synthetic Nuclei of Xenopus. Transcription Elongation and Termination: Factors and Complexes: S. Borukhov and A. Goldfarb, Purification and Assay of Escherichia coli Transcript Cleavage Factors GreA and GreB. M. Kashlev, E. Nudler, K.Severinov, S. Borukhov, N. Komissarova, and A. Goldfarb, His-Tagged Polymerase of Escherichia coli and Transcription in Solid Phase. R. Landick, D. Wang, and C.L. Chan, Quantitative Analysis of Transcriptional Pausing by Escherichia coli Polymerase: his Leader Pause Site as Paradigm. W. Nowatzke, L. Richardson, and J.P. Richardson, Purification of Transcription Termination Factor Rho from Escherichia coli and Micrococcus luteus. A. Das, S. Barek, B. Ghosh, and W. Whalen, Immunoprinting: A Technique to Study Dynamic Protein–Nucleic Acid Interactions within the Transcription Elongation Complex. A. Das, M. Pal, J.G. Meno, W.N. Lalen, K. Wolska, R. Crossley, W. Rees, P.H. von Hippel, N. Constantino, D. Court, M.Mazzulla, A.S. Altieri, R.A. Byrd, S. Chattopadhyay, J. de Vito, and B. Ghosh, Components of a Multiprotein-RNA Complex That Controls Transcription Elongation in Escherichia coli Phage Lambda. M.M. Hanna, Photochemical Cross-linking Analysis of Protein–Nucleic Acid Interactions in Escherichia coli Transcription Complexes from the Lambda PR1 Promoter. A.M. Edwards and C.M. Kane, Identifying Regulators of Transcript Elongation in Eukaryotes. A. Pause, T. Aso, W.M. Linehan, J.W. Conaway, R.C. Conaway, and R.D. Klausner, Interaction of the VHL Tumor Suppressor Gene Product with Elongin. Specialized Technique: T.D. Schneider, Reading of DNA Sequence Logos: Prediction of Major Groove Binding by Information Theory. D. Chatterjee and V. Gopal, Fluorescence Spectroscopy Analysis of Active and Regulatory Sties of RNA Polymerase. M. Hsieh and M. Brenowitz, Quantitative Kinetics Footprinting: Protein–DNA Association Reactions. T. Heyduk, Y.Ma, H. Tang, and R.H. Ebright, Fluorescence Anisotropy: Assay for Protein–DNA and Protein–Protein Interaction. K.A. Sharif, J. Luo, and J.S. Krakow, Epitope Mapping of Monoclonal Antibodies Directed against Subunits of RNA Polymerase ofEscherichia coli. N.E. Thompson and R.R. Burgess, Immunoaffinity Purification of Wheat Germ RNA Polymerase II and Transcription Factors Using Polyol-Responsive Monoclonal Antibodies. Author Index. Subject Index.


The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Basal transcription and regulation of transcription: transcription factors, activators, and repressors

  • Chromatin as templates for transcription
  • Transcription elongation and termination: factors and complexes
  • Specialized techniques


Biochemists, microbiologists, molecular biologists, geneticists, and those in the pharmaceutical industry.


No. of pages:
© Academic Press 1996
14th October 1996
Academic Press
eBook ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

Sankar Adhya

Affiliations and Expertise

National Cancer Institute, Laboratory of Molecular Biology, National Institutes of Health