Description

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Basal transcription and regulation of transcription: transcription factors, activators, and repressors * Chromatin as templates for transcription * Transcription elongation and termination: factors and complexes * Specialized techniques

Readership

Biochemists, microbiologists, molecular biologists, geneticists, and those in the pharmaceutical industry.

Table of Contents

Basal Transcription and Regulation of Transcription: Transcription Factors, Activators, and Repressors: H.E. Choy, A Complete Ribosome-Free in Vitro Transcription System Responsive to Extract of Escherichiacoli. A. Miller, M. Choi, M.A. Glucksmann-Kuis, X. Dai, and L.B. Rothman-Denes, Single-Stranded DNA-Binding Proteins as Transcriptional Activators. S.E. Hasnain, S. Habib, A. Jain, S. Burma, and B. Mukherjee, Host Factor with Single-Stranded DNA-Binding Activity Involved in Transcription from Baculovirus Polyhedrin Promoter. N. Goosen, P. van Ulsen, L. Zulianello, and P. van de Putte, Transcription Activiation by Histone-like Protein IHF. D.M. Hinton, R. March-Amegadzie, J.S. Gerber, and M. Sharma, Bacteriophage T4 Middle Transcription System: T4-Modified RNA Polymerase, AsiA (Sigma-70 Binding Protein), and Transcriptional Activator MotA. H. Ge, E. Martinez, C.-M. Chiang, and R.G. Roeder, Activator-Dependent Transcription by Mammalian RNA Polymerase II: In Vitro Reconstitution with General Transcription Factors and Cofactors. E. Maldonado, R. Drapkin, and D. Reinberg, Purification of Human RNA Polymerase II and General Transcription Factors. W.-C. Yeh, J. Hou, and S.L. McKnight, Purification and Characterization of Gene-Specific Transcription Factors from Rat Liver: c/EBP, GABP, and IL-4 Stat. M. Zhong, J. Wisniewski, M. Fritsch, M. Mizuguchi, A. Orosz, P. Jedlicka, and C. Wu, Purification of Heat ShockTranscription Factor of Drosophila. J. Greenblatt and C.J. Ingles, Interaction between Acidic Transcriptional Activation Domains of Activator Protein VP16 Herpes Simplex Virus and TRIID. M. Carey, D.Tantin, T. Chi, R. Hori, and S. Pyo, Biochemical Mechanism of Transcriptional Activation by Ga14-VP16. R. Valcarcel and H.G. Stunnenberg, Retinoid-Dependent in Vitro Transcription. D. Thanos and T. Maniatis, In Vitro

Details

No. of pages:
566
Language:
English
Copyright:
© 1996
Published:
Imprint:
Academic Press
Print ISBN:
9780121821753
Electronic ISBN:
9780080883878

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY