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RNA Polymerase and Associated Factors, Part A - 1st Edition - ISBN: 9780121821746, 9780080883861

RNA Polymerase and Associated Factors, Part A, Volume 273

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: Sankar Adhya
eBook ISBN: 9780080883861
Imprint: Academic Press
Published Date: 16th September 1996
Page Count: 377


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Table of Contents

Promoter Elements and RNA Polymerase Components: G.N. Gussin and J.S. Fassler, Promoters and the Basal Transcriptional Machinery in Eurbacteria and Eukaryotes: Concepts, Definitions, and Analogies. G.Z. Hertz andG.D. Stormo, Escherichia coli Promoter Sequences: Analysis and Prediction. Biochemical Assays of Transcription Initiation: G.N. Gussin, Kinetic Analysis of RNAP-Promoter Interactions. L.M. Hsu, Quantitative Pararmeters for Promoter Clearance. F. Qi, C. Liu, L.S. Heath, and C.L. Turnbough, Jr., In Vitro Assay for Reiterative Transcription during Transcriptional Initiation by Escherichia coli RNA Polymerase. H. Zhang, N. Iler, and C. Abate, Rigorous and Quantitative Assay of Transcription in Vitro. J.D. Gralla, Global Steps during Initiation by RNA Polymerase II. S.G. Roberts and M.R. Green, Purification and Analysis of Functional Preinitiation Complexes. RNA Polymerase and Its Subunits in Prokaryotes: N. Fujita and A. Ishihama, Reconstitution of RNA Polymerase. H. Tang, Y. Kim, K. Severinov, A. Goldfarb, and R.H. Ebright, Escherichia coli RNA Polymerase Holoenzyme: Rapid Reconstitution for Recombinant alpha, beta, beta, and sigma Subunits. A.J. Dombrowski, Sigma Factors: Purification and DNA Binding. R.R. Burgess, Purification of Overproduced Escherichia coli RNA Polymerase Sigma Factors by Solubilizing Inclusion Bodies and Refolding from Sarkosyl. K.M. Tatti and C.P. Moran, Jr., RNA Polymerase Sigma Factors of Bacillus subtilis: Purification and Characterization. RNA Polymerase and Associated Factors from Eukaryotes: R.G. Roeder, Nuclear RNA Polymerases: Role of General Initiation Factors and Cofactors in Eukaryotic Transcription. Y. Li, S. Bjorklund, Y.-J. Kim, and R.D. Kornberg, Yeast RNA Polymerase II Holoenzyme. A.J. Koleske, D.M. Chao, and R.A. Young, Purification of Yeast RNA Polymerase II Holoenzymes. M.E. Dahmus, Phosphorylation of Mammalian RNA Polymerase II. R.C. Conaway, D. Reiner, K.P. Garrett, W. Powell, and J.W. Conaway, Purification of RNA Polymerase II General Transcription Factors from Rat Liver. J.-L. Chen and R. Tijan, Reconstitution of TAP/TBP Complexes for in Vitro Transcription. B. de Crombrugghe and S.N. Maity, Purification, Characterization, and Role of CCAAT Binding Protein CBF in Transcription. I. Grummt and A. Schnapp, Purification, Assay, and Properties of RNA Polymerase I andClass I Specific Transcription Factors in Mouse. J. Huet, N. Manaud, G. Dieci, G. Peyroche, C. Conesa, O. Lefebre, A. Ruet, M. Riva, and A. Sentenac, RNA Polymerase III and Class III Transcription Factors from Saccharomyces cerevisiae. M. Sugiura and H. Fan, Basal and Activated in Vitro Transcription in Plants by RNA Polymerases II and III. Genetic Analysis of Transcription and Its Regulation: R. Hakenbeck and J.B. Stock, Approaches to Analysis of Novel Two-Component Signal Transduction Systems Involved in Transcriptional Regulation. D.J. Jin and Y.N. Zhou, Mutational Analysis of Structure@#150;Function Relationship of RNA Polymerase of Escherichia coli. V.K. Jain, Vectors with Bidirectional Reporter Genes for Studying Divergent Promoters. C. Bai and S.J. Elledge, Gene Identification Using the Yeast Two-Hybrid System. Author Index. Subject Index.


The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Promoter elements and RNA polymerase components

  • RNA polymerase and its subunits in prokaryotes
  • Biochemical assays of transcription initiation
  • RNA polymerase and associated factors from eukaryotes
  • Genetic analysis of transcription and its regulation


Biochemists, microbiologists, molecular biologists, geneticists, and those in the pharmaceutical industry.


No. of pages:
© Academic Press 1996
16th September 1996
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

Sankar Adhya

Affiliations and Expertise

National Cancer Institute, Laboratory of Molecular Biology, National Institutes of Health