The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Promoter elements and RNA polymerase components * RNA polymerase and its subunits in prokaryotes * Biochemical assays of transcription initiation * RNA polymerase and associated factors from eukaryotes * Genetic analysis of transcription and its regulation


Biochemists, microbiologists, molecular biologists, geneticists, and those in the pharmaceutical industry.

Table of Contents

Promoter Elements and RNA Polymerase Components: G.N. Gussin and J.S. Fassler, Promoters and the Basal Transcriptional Machinery in Eurbacteria and Eukaryotes: Concepts, Definitions, and Analogies. G.Z. Hertz andG.D. Stormo, Escherichia coli Promoter Sequences: Analysis and Prediction. Biochemical Assays of Transcription Initiation: G.N. Gussin, Kinetic Analysis of RNAP-Promoter Interactions. L.M. Hsu, Quantitative Pararmeters for Promoter Clearance. F. Qi, C. Liu, L.S. Heath, and C.L. Turnbough, Jr., In Vitro Assay for Reiterative Transcription during Transcriptional Initiation by Escherichia coli RNA Polymerase. H. Zhang, N. Iler, and C. Abate, Rigorous and Quantitative Assay of Transcription in Vitro. J.D. Gralla, Global Steps during Initiation by RNA Polymerase II. S.G. Roberts and M.R. Green, Purification and Analysis of Functional Preinitiation Complexes. RNA Polymerase and Its Subunits in Prokaryotes: N. Fujita and A. Ishihama, Reconstitution of RNA Polymerase. H. Tang, Y. Kim, K. Severinov, A. Goldfarb, and R.H. Ebright, Escherichia coli RNA Polymerase Holoenzyme: Rapid Reconstitution for Recombinant alpha, beta, beta, and sigma Subunits. A.J. Dombrowski, Sigma Factors: Purification and DNA Binding. R.R. Burgess, Purification of Overproduced Escherichia coli RNA Polymerase Sigma Factors by Solubilizing Inclusion Bodies and Refolding from Sarkosyl. K.M. Tatti and C.P. Moran, Jr., RNA Polymerase Sigma Factors of Bacillus subtilis: Purification and Characterization. RNA Polymerase and Associated Factors from Eukaryotes: R.G. Roeder, Nuclear RNA Polymerases: Role of General Initiation Factors and Cofactors in Eukaryotic Transcription. Y. Li, S. Bjorklund, Y.-J. Kim, and R.D. Kornberg, Yeast RNA Polymerase II Holoenzyme. A.J. Koleske


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© 1996
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY