RNA Modification Enzymes

RNA Modification Enzymes

1st Edition - September 11, 2021
This is the Latest Edition
  • Editor: Jane Jackman
  • eBook ISBN: 9780128235867
  • Hardcover ISBN: 9780128235850

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Description

RNA Modification Enzymes, Volume 659 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics, including Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis, Development of RNA modification mapping pipelines for high-throughput sequencing approaches, AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications, Facile detection of RNA phospho-methylation in cells, Detection and analysis of glycosylated queuosine modifications, A comprehensive pipeline for analysis of RNA 3’-end modification, Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on the RNA Modification Enzymes

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Preface
    Jane E. Jackman
    1. Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis
    Balasubrahmanyam Addepalli
    2. Development of RNA modification mapping pipelines for high-throughput sequencing approaches
    Todd Lowe
    3. AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications
    Yuri Motorin
    4. Facile detection of RNA phospho-methylation in cells
    Blerta Xhemalce
    5. Detection and analysis of glycosylated queuosine modifications
    Tao Pan
    6. A comprehensive pipeline for analysis of RNA 3’-end modification
    Inna Afasizheva
    7. Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry
    Stefanie Kellner
    8. Direct sequencing (ONT) to detect m6A modification
    9. RNA immunoprecipitation to identify in vivo targets of RNA editing and modifying enzymes
    Heather A. Hundley
    10. AdoMet-based labeling of RNA
    Andrea Rentmeister
    11. Coupling crosslinking and RNA modification sequencing for identifying RNA modification interacting proteins
    Juan Alfonzo
    12. Characterization of codon-specific translation defects using ribosome profiling
    Sebastian A. Leidel
    13. Partially modified tRNAs for the study of tRNA maturation and function
    Ute Kothe
    14. In vitro assays for complex multi-step RNA modification reactions
    Dirk Iwata-Reuyl
    15. Transient kinetic analysis for studying RNA modification enzyme mechanisms
    Jane E. Jackman
    16. High-throughput detection and analysis of pseudouridine synthase activities
    Wendy Gilbert
    17. Activity assays for wobble inosine modification in tRNA
    Dragony Fu
    18. Targeting RNA with site-directed RNA Editing
    Josh Rosenthal
    19. RNA pull-down assays for distinguishing tRNA modification substrate specificities
    Eric Phizicky
    20. Assessing the consequences of mRNA modifications on protein synthesis using in vitro translation assays
    Kristin Koutmou
    21. Mass spectrometry based methods for studying mRNA 5’-cap modifications
    Tsutomu Suzuki
    22. CLIP-Seq to identify targets and interactions of RNA modification enzymes
    Fange Liu
    23. CRISPR-mediated genome editing targeting RNA modification enzymes
    Douglas Bernstein

Product details

  • No. of pages: 474
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: September 11, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780128235867
  • Hardcover ISBN: 9780128235850

About the Serial Volume Editor

Jane Jackman

Jane E. Jackman, Professor, Department of Chemistry and Biochemistry, Director and Graduate Studies Committee Chair, The Ohio State Biochemistry Program, Dept of Chemistry and Biochemistry, Columbus, USA

Affiliations and Expertise

Professor, Department of Chemistry and Biochemistry, The Ohio State University, Columbus, USA