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Jane E. Jackman
1. Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis
2. Development of RNA modification mapping pipelines for high-throughput sequencing approaches
3. AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications
4. Facile detection of RNA phospho-methylation in cells
5. Detection and analysis of glycosylated queuosine modifications
6. A comprehensive pipeline for analysis of RNA 3’-end modification
7. Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry
8. Direct sequencing (ONT) to detect m6A modification
9. RNA immunoprecipitation to identify in vivo targets of RNA editing and modifying enzymes
Heather A. Hundley
10. AdoMet-based labeling of RNA
11. Coupling crosslinking and RNA modification sequencing for identifying RNA modification interacting proteins
12. Characterization of codon-specific translation defects using ribosome profiling
Sebastian A. Leidel
13. Partially modified tRNAs for the study of tRNA maturation and function
14. In vitro assays for complex multi-step RNA modification reactions
15. Transient kinetic analysis for studying RNA modification enzyme mechanisms
Jane E. Jackman
16. High-throughput detection and analysis of pseudouridine synthase activities
17. Activity assays for wobble inosine modification in tRNA
18. Targeting RNA with site-directed RNA Editing
19. RNA pull-down assays for distinguishing tRNA modification substrate specificities
20. Assessing the consequences of mRNA modifications on protein synthesis using in vitro translation assays
21. Mass spectrometry based methods for studying mRNA 5’-cap modifications
22. CLIP-Seq to identify targets and interactions of RNA modification enzymes
23. CRISPR-mediated genome editing targeting RNA modification enzymes
RNA Modification Enzymes, Volume 659 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of related topics, including Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis, Development of RNA modification mapping pipelines for high-throughput sequencing approaches, AlkAniline-Seq for high-resolution mapping RNA m7G and m3C modifications, Facile detection of RNA phospho-methylation in cells, Detection and analysis of glycosylated queuosine modifications, A comprehensive pipeline for analysis of RNA 3’-end modification, Analysis of the epitranscriptome with ion-pairing reagent free oligonucleotide mass spectrometry, and more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
- Updated release includes the latest information on the RNA Modification Enzymes
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- No. of pages:
- © Academic Press 2021
- 1st October 2021
- Academic Press
- Hardcover ISBN:
Jane E. Jackman, Professor, Department of Chemistry and Biochemistry, Director and Graduate Studies Committee Chair, The Ohio State Biochemistry Program, Dept of Chemistry and Biochemistry, Columbus, USA
Professor, Department of Chemistry and Biochemistry, The Ohio State University, Columbus, USA
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