**Fully revised with five new chapters, a new appendix, and nine chapters with major expansions** CHAPTER 1: RNA and the Cellular Biochemistry RevisitedCHAPTER 2: RNA Isolation StrategiesCHAPTER 3: The Truth about TissuesCHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW)CHAPTER 5: Isolation of Polyadenylated RNACHAPTER 6: Quality Control for RNA Preparations (EXPANDED)CHAPTER 7: Resilient RibonucleasesCHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED)CHAPTER 9: Electrophoresis of RNA (EXPANDED)CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED)CHAPTER 11: Northern AnalysisCHAPTER 12: Nucleic Acid Probe TechnologyCHAPTER 13: Practical Nucleic Acid HybridizationCHAPTER 14: Principles of Detection (EXPANDED)CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection CHAPTER 16: Analysis of Nuclear RNA (EXPANDED)CHAPTER 17: cDNA Synthesis (EXPANDED)CHAPTER 18: RT-PCR (NEW)CHAPTER 19: Quantitative PCR Techniques CHAPTER 20: Functional Genomics and Transcript Profiling (NEW)CHAPTER 21: High-Throughput Analysis of Gene Expression CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW)CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED)CHAPTER 24: Genomes, Transcriptomes, Proteomes, and BioinformaticsCHAPTER 25: An RNA ParadigmAPPENDIX A: Maintaining Complete and Accurate RecordsAPPENDIX B: Converting Mass to Moles (NEW)APPENDIX C: Useful Stock Solutions for the Molecular BiologistAPPENDIX D: Phenol PreparationAPPENDIX E: Disposal of Ethidium Bromide and SYBR Green SolutionsAPPENDIX F: DNase I Removal of DNA From An RNA SampleAPPENDIX G: DNase Incubation to Remove RNA from a DNA SampleAPPENDIX H: Deionization of Formamide, Formaldehyde, and GlyoxalAPPENDIX I: Silanizing Centrifuge Tubes and GlasswareAPPENDIX J: Trypsinization Protocol for Anchorage-Dependent CellsAPPENDIX K: Isolation of High-Molecular-Weight DNA by Salting-OutAPPENDIX L: Electrophoresis: Principles, Parameters, and SafetyAPPENDIX M: Polyacrylamide Gel ElectrophoresisAPPENDIX N: Dot Blot Analysis (NEW)APPENDIX O: Centrifugation as a Mainstream Tool for the Molecular BiologistAPPENDIX P: Selected Suppliers of Equipment, Reagents, and ServicesAPPENDIX Q: Useful SI UnitsAPPENDIX R: Common AbbreviationsAPPENDIX S: Trade Citations