RNA Methodologies

RNA Methodologies

Laboratory Guide for Isolation and Characterization

4th Edition - August 31, 2009

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  • Author: Robert E. Farrell, Jr.
  • eBook ISBN: 9780080884950

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Description

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.

Key Features

* 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary

* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center

* Includes classic and contemporary techniques useful for all labs

Readership

Researchers, graduate students and lab technicians in the fields of molecular biology, cell biology, and biochemical genetics

Table of Contents

  • **Fully revised with five new chapters, a new appendix, and nine chapters with major expansions**

    CHAPTER 1: RNA and the Cellular Biochemistry Revisited
    CHAPTER 2: RNA Isolation Strategies
    CHAPTER 3: The Truth about Tissues
    CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW)
    CHAPTER 5: Isolation of Polyadenylated RNA
    CHAPTER 6: Quality Control for RNA Preparations (EXPANDED)
    CHAPTER 7: Resilient Ribonucleases
    CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED)
    CHAPTER 9: Electrophoresis of RNA (EXPANDED)
    CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED)
    CHAPTER 11: Northern Analysis
    CHAPTER 12: Nucleic Acid Probe Technology
    CHAPTER 13: Practical Nucleic Acid Hybridization
    CHAPTER 14: Principles of Detection (EXPANDED)
    CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection
    CHAPTER 16: Analysis of Nuclear RNA (EXPANDED)
    CHAPTER 17: cDNA Synthesis (EXPANDED)
    CHAPTER 18: RT-PCR (NEW)
    CHAPTER 19: Quantitative PCR Techniques
    CHAPTER 20: Functional Genomics and Transcript Profiling (NEW)
    CHAPTER 21: High-Throughput Analysis of Gene Expression
    CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW)
    CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED)
    CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics
    CHAPTER 25: An RNA Paradigm

    APPENDIX A: Maintaining Complete and Accurate Records
    APPENDIX B: Converting Mass to Moles (NEW)
    APPENDIX C: Useful Stock Solutions for the Molecular Biologist
    APPENDIX D: Phenol Preparation
    APPENDIX E: Disposal of Ethidium Bromide and SYBR Green Solutions
    APPENDIX F: DNase I Removal of DNA From An RNA Sample
    APPENDIX G: DNase Incubation to Remove RNA from a DNA Sample
    APPENDIX H: Deionization of Formamide, Formaldehyde, and Glyoxal
    APPENDIX I: Silanizing Centrifuge Tubes and Glassware
    APPENDIX J: Trypsinization Protocol for Anchorage-Dependent Cells
    APPENDIX K: Isolation of High-Molecular-Weight DNA by Salting-Out
    APPENDIX L: Electrophoresis: Principles, Parameters, and Safety
    APPENDIX M: Polyacrylamide Gel Electrophoresis
    APPENDIX N: Dot Blot Analysis (NEW)
    APPENDIX O: Centrifugation as a Mainstream Tool for the Molecular Biologist
    APPENDIX P: Selected Suppliers of Equipment, Reagents, and Services
    APPENDIX Q: Useful SI Units
    APPENDIX R: Common Abbreviations
    APPENDIX S: Trade Citations

Product details

  • No. of pages: 744
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: August 31, 2009
  • Imprint: Academic Press
  • eBook ISBN: 9780080884950

About the Author

Robert E. Farrell, Jr.

Dr. Robert Farrell is a bench-current scientist who has 35 years of experience working with RNA in the study of transcriptional and posttranscriptional regulation of gene expression in a variety of model systems. He is also experienced in animal cell culture methods. Prior to joining the faculty at Penn State University, he operated a biotech education and service firm, winning the 1998 Small Business Contractor of the Year award from the U.S. Department of Agriculture. He is the recipient of campus- and college-wide awards for excellence in teaching, and has extensive experience running RNA and specialized biotechnology hands-on laboratory training programs all over the world. He often serves as a consultant within the pharmaceutical and biotech industries. Dr. Farrell received his Ph.D. and M.S. degrees from The Catholic University of America and his B.S. in Biology from Providence College. Dr. Farrell currently serves as the campus academic officer at Penn State York.

Affiliations and Expertise

Bench-Current Scientist, Pennsylvania State University, York, PA, USA

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