RNA Interference

RNA Interference

1st Edition - February 7, 2005

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  • Authors: David Engelke, John Rossi
  • eBook ISBN: 9780080455396

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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent.

Key Features

* One of the most highly respected publications in the field of biochemistry since 1955
* Frequently consulted, and praised by researchers and reviewers alike
* Truly an essential publication for anyone in any field of the life sciences


Biochemists, cell biologists, molecular biologists, geneticists

Table of Contents

  • Transgene-induced RNA Interference as a Tool for Plant Functional Genomics
    Constructs and Methods for hairpin RNA-mediated Gene Silencing in Plants
    RNA interference in Caenorhabditis elegans
    High-throughput RNAi screens in Drosophila tissue culture cells
    Mechanistic insights aid computational siRNA design
    Novel methods for expressing RNAi in human cells
    Delivery of Small Interfering RNA to Mammalian Cells in Culture Using Cationic Lipid/Polymer-Based Transfection Reagents
    The subcellular distribution of siRNA: directed delivery through RNA polymerase III expression cassettes and localization using in situ hybridization
    Viral delivery of recombinant shRNAs
    Downregulation of cellular genes by PCR cassettes expressing short interfering RNAs
    Using short hairpin RNA expression vectors to study mammalian neural development
    Analysis of T-cell development using siRNA to knockdown protein expression
    Viral delivery of recombinant siRNA expression: lentivirus
    Paradigms for Conditional Expression of RNAi Molecules for use Against Viral Targets
    High throughput RNAi strategies for target discovery and validation using synthetic siRNAs: functional genomics investigations of biological pathways
    RNA interference in vivo: towards synthetic siRNA based therapeutics
    Labeling and characterization of small RNAs associated with the RNAi effector complex, RITS
    RNAi spreading in C. elegans
    Human Dicer: Purification, properties, and interaction with PAZ PIWI domain proteins
    Delivery of siRNA and siRNA Expression Constructions to Adult Mammals Using Hydrodynamic Intravascular Injection
    Analysis of siRNA function in RNAi using Drosophila Embryo extracts and Schneider cells
    Using RNA polymerase II to transcribe artificial microRNAs
    Adeno-associated virus vectors for short hairpin RNA expression
    Simple, Robust Strategies for Generating ddRNAi Constructs

Product details

  • No. of pages: 496
  • Language: English
  • Copyright: © Academic Press 2005
  • Published: February 7, 2005
  • Imprint: Academic Press
  • eBook ISBN: 9780080455396

About the Authors

David Engelke

Affiliations and Expertise

University of Michigan, Ann Arbor, U.S.A.

John Rossi

Affiliations and Expertise

City of Hope's Beckman Research Institute, Duarte, California, U.S.A.

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