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RNA Helicases - 1st Edition - ISBN: 9780123965462, 9780123972293

RNA Helicases, Volume 511

1st Edition

Serial Volume Editor: Eckhard Jankowsky
Hardcover ISBN: 9780123965462
eBook ISBN: 9780123972293
Imprint: Academic Press
Published Date: 22nd June 2012
Page Count: 584
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Table of Contents

  1. Analysis of Duplex Unwinding by RNA Helicases Using Stopped Flow Fluorescence Spectroscopy
  2. Andrea A. Putnam & Eckhard Jankowsky

  3. Analyzing ATP Utilization by DEAD-box RNA Helicases Using Kinetic and Equilibrium Methods
  4. Michael J. Bradley & Enrique M. De La Cruz

  5. Oxygen Isotopic Exchange Probes of ATP Hydrolysis by RNA Helicases
  6. David D. Hackney

  7. Conformational Changes of DEAD-box Helicases Monitored by Single Molecule Fluorescence Resonance Energy Transfer
  8. Alexandra Z. Andreou & Dagmar Klostermeier

  9. RNA Catalysis as a Probe for Chaperone Activity of DEAD-box Helicases
  10. Jeffrey P. Potratz & Rick Russell

  11. Molecular Mechanics of RNA Translocases
  12. Steve C. Ding & Anna Marie Pyle

  13. Analysis of Helicase-RNA Interactions Using Nucleotide Analog Interference Mapping
  14. Annie Schwartz, Makhlouf Rabhi, Emmanuel Margeat & Marc Boudvillain

  15. Crystallization and X-ray Structure Determination of an RNA-dependent Hexameric Helicase
  16. Nathan D. Thomsen & James M. Berger

  17. Structural Analysis of RNA Helicases with Small-angle X-ray Scattering
  18. Manja A. Behrens, Yangzi He, Cristiano L. P. Oliveira, Gregers R. Andersen, Jan Skov Pedersen & Klaus H. Nielsen

  19. Analysis of Co-factor Effects on RNA Helicases
  20. Crystal Young & Katrin Karbstein

  21. Analysis of DEAD-box Proteins in mRNA Export
  22. Ben Montpetit, Markus A. Seeliger & Karsten Weis

  23. Biochemical Characterization of the RNA Helicase UPF1 Involved in Nonsense-mediated mRNA Decay
  24. Francesca Fiorini, Fabien Bonneau & Hervé Le Hir

  25. Identification of RNA Helicase Target Sites by UV Crosslinking and Analysis of cDNA (CRAC)
  26. Markus T. Bohnsack, David Tollervey & Sander Granneman

  27. In Vivo Approaches to Dissecting the Function of RNA Helicases in Eukaryotic Ribosome Assembly
  28. David C. Rawling & Susan J. Baserga

  29. Analysis of RNA Helicases in P-bodies and Stress Granules
  30. Angela Hilliker

  31. DEAD box RNA Helicases as Transcription Co-factors
  32. Frances V. Fuller-Pace & Samantha M. Nicol

  33. DEAD-box RNA Helicases in Gram-positive RNA-decay
  34. Peter Redder & Patrick Linder

  35. RNA Helicases in Cyanobacteria: Biochemical and Molecular Approaches
  36. George W. Owttrim

  37. Determination of Host RNA Helicases Activity in Viral Replication
  38. Amit Sharma & Kathleen Boris-Lawrie

  39. Inhibitors of Translation Targeting Eukaryotic Translation Initiation Factor 4A
  40. Regina Cencic, Gabriela Galicia-Vázquez & Jerry Pelletier

  41. Identification and Analysis of Inhibitors Targeting the Hepatitis C Virus NS3 Helicase

Alicia M. Hanson, John J. Hernandez, William R. Shadrick, & David N. Frick


This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases.

Key Features

  • This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases
  • The contributions in this volume cover the broad scope of methods in the research on these enzymes


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


No. of pages:
© Academic Press 2012
22nd June 2012
Academic Press
Hardcover ISBN:
eBook ISBN:

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About the Serial Volume Editor

Eckhard Jankowsky