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RNA and Protein Synthesis
- 1st Edition - January 28, 1981
- Editor: Kivie Moldave
- Language: English
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 4 2 3 4 - 2
RNA and Protein Synthesis is a compendium of articles dealing with the assay, characterization, isolation, or purification of various organelles, enzymes, nucleic acids,… Read more
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Request a sales quoteRNA and Protein Synthesis is a compendium of articles dealing with the assay, characterization, isolation, or purification of various organelles, enzymes, nucleic acids, translational factors, and other components or reactions involved in protein synthesis. One paper describes the preparatory scale methods for the reversed-phase chromatography systems for transfer ribonucleic acids. Another paper discusses the determination of adenosine- and aminoacyl adenosine-terminated sRNA chains by ion-exclusion chromatography. One paper notes that the problems involved in preparing acetylaminoacyl-tRNA are similar to those found in peptidyl-tRNA synthesis, in particular, to the lability of the ester bond between the amino acid and the tRNA. Another paper explains a new method that will attach fluorescent dyes to cytidine residues in tRNA; it also notes the possible use of N-hydroxysuccinimide esters of dansylglycine and N-methylanthranilic acid in the described method. One paper explains the use of membrane filtration in the determination of apparent association constants for ribosomal protein-RNS complex formation. This collection is valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and investigators working with enzymes.
List of Contributors
Foreword
Preface
Section I. Transfer RNA
1. Reversed-Phase Chromatography Systems for Transfer Ribonucleic Acids-Preparatory-Scale Methods
2. Miniature Reversed-Phase Chromatography Systems for the Rapid Resolution of Transfer RNA's and Ribosomal RNA's
3. Transfer RNA Isoacceptors in Cultured Chinese Hamster Ovary Cells
4. The Use of BD-Cellulose in Separating Transfer RNA's
5. Fractionation of Rabbit Liver Methionyl-tRNA Species
6. RNase Hydrolysis of Aminoacyl-sRNA to Aminoacyl Adenosine
7. Determination of Adenosine- and Aminoacyl Adenosine-Terminated sRNA Chains by Ion-Exclusion Chromatography
8. Enzymic Modification of the C-C-A Terminus of tRNA
9. Methods for Determining the Extent of tRNA Aminoacylation in Vivo in Cultured Mammalian Cells
10. The Chemical Preparation of Acetylaminoacyl-tRNA
11. The Synthesis of Oligopeptidyl-tRNA
12. Preparation of Aminoacyl-Oligonucleotides and Their Binding to Ribosomes
13. Chemical Modifications of Amino Acids Esterified to tRNA (Other Than Acylations)
14. Use of in Vitro 32P Labeling in the Sequence Analysis of Nonradioactive tRNAs
15. A New Method for Attachment of Fluorescent Probes to tRNA
16. Attachment of Cross-Linking Reagents to tRNA for Protein Affinity Labeling Studies
17. Enzymatic Synthesis of Trinucleoside Diphosphates of Known Sequence
18. Use of Purified Isoacceptor tRNAs for the Study of Codon-Anticodon Recognition in Vitro with Sequenced Natural Messenger RNA
19. Rabbit Liver tRNA Nucleotidyltransferase
20. Bacterial tRNA Methyltransferases
21. Assay for AA-tRNA Recognition by the EFTu-GTP Complex of Escherichia coli
22. Mapping the Structure of Specific Protein-Transfer RNA Complexes by a Tritium Labeling Method
Section II. Aminoacyl-tRNA Synthetases
23. Aminoacyl-tRNA Synthetase Complex from Rat Liver
24. Kinetic Techniques for the Investigation of Amino Acid:tRNA Ligases (Aminoacyl-tRNA Synthetases, Amino Acid Activating Enzymes)
25. Kinetics of Aminoacyl-tRNA Synthetases Catalyzed ATP-PP Exchange
26. Applications of Kinetic Methods to AminoacyltRNA Synthetases
Section III. Polypeptide Chain Initiation
27. Purification of Protein Synthesis Initiation Factors IF-1, IF-2, and IF-3 from Escherichia coli
28. Protein Synthesis Initiation Factors from Rabbit Reticulocytes: Purification, Characterization, and Radiochemical Labeling
29. Initiation Factors for Protein Synthesis from Wheat Germ
30. Assay of the Binding of the Ternary Complex Met-tRNAf · eIF-2 · GTP to the 40 S Ribosomal Subunit by Sucrose Gradient and CsCl Gradient Analysis
31. Prokaryotic Ribosome Binding Sites
32. Characterization of Translational Initiation Regions from Eukaryotic Messenger RNAs
33. Determination of the Number of Ribosomal Binding Sites on the RNAs of Eukaryotic Viruses
34. Methods for Analyzing Messenger Discrimination in Eukaryotic Initiation Factors
35. Isolation of Protein Kinases from Reticulocytes and Phosphorylation of Initiation Factors
Section IV. Polypeptide Chain Elongation
36. Isolation of the Protein Synthesis Elongation Factors EF-Τu, EF-Ts, and EF-G from Escherichia coli
37. Preparation of Homogeneous Escherichia coli Translocation Factor G
38. The Binding of Escherichia coli Elongation Factor G to the Ribosome
39. GTP-Dependent Binding of Aminoacyl-tRNA to Escherichia coli Ribosomes
40. Polypeptide Chain Elongation Factors from Pig Liver
41. Separation of Cytoplasmic and Mitochondrial Elongation Factors from Yeast
42. Soluble Protein Factors and Ribosomal Subunits from Yeast. Interaction with Aminoacyl-tRNA
43. Separation and Characterization of Yeast Elongation Factors
Section V. Polypeptide Chain Termination
44. Release Factors: In Vitro Assay and Purification
45. Mammalian Release Factor: In Vitro Assay and Purification
Section VI. Ribosomes
46. Sedimentation Velocity Analysis in Accelerating Gradients
47. Preparation of Ribosomal Subunits by Large-Scale Zonal Centrifugation
48. A Gel Electrophoretic Separation of Bacterial Ribosomal Subunits with and without Protein SI
49. Reconstitution of Ribosomes from Subribosomal Components
50. Total Reconstitution of 50 S Subunits from Escherichia coli Ribosomes
51. Purification of Ribosomal Proteins from Escherichia coli
52. Purification of Ribosomal Proteins from Escherichia coli under Nondenaturing Conditions
53. Two-Dimensional Polyacrylamide Gel Electrophoresis for Separation of Ribosomal Proteins
54. A Modified Two-Dimensional Gel System for the Separation and Radioautography of Microgram Amounts of Ribosomal Proteins
55. Analytical Methods for Ribosomal Proteins of Rat Liver 40 S and 60 S Subunits by "Three-Dimensional" Acrylamide Gel Electrophoresis
56. Cross-Linking of Ribosomes Using 2-Iminothiolane (Methyl 4-Mercaptobutyrimidate) and Identification of Cross-Linked Proteins by Diagonal Polyacrylamide/Sodium Dodecyl Sulfate Gel Electrophoresis
57. Photoaffinity Labeling of Ribosomes
58. Radioactive Chemical Labeling of Ribosomal Proteins and Translational Factors in Vitro
59. Peptidyl-Puromycin Synthesis on Polyribosomes from Escherichia coli
60. Separation of Large Quantities of Eukaryotic Ribosomal Subunits by Zonal Ultracentrifugation
61. Two-Dimensional Polyacrylamide Gel Electrophoresis of Eukaryotic Ribosomal Proteins
62. Isotopic Labeling and Analysis of Phosphoproteins from Mammalian Ribosomes
63. Phosphorylation of Ribosomal Proteins: Preparation of Rat Liver Ribosomal Protein Kinases. Assay of the Phosphorylation of Ribosomal Subunit Proteins. Assay of the Function of Phosphorylated Ribosomes
64. Protein-RNA Interactions in the Bacterial Ribosome
65. The Use of Membrane Filtration to Determine Apparent Association Constants for Ribosomal Protein-RNA Complex Formation
66. Preparation and Analysis of Mitochondrial Ribosomes
Index
- No. of pages: 830
- Language: English
- Edition: 1
- Published: January 28, 1981
- Imprint: Academic Press
- eBook ISBN: 9780323142342
KM
Kivie Moldave
Affiliations and expertise
Department of Molecular Biology and Biochemistry, University of California, Irvine, California, U.S.A.Read RNA and Protein Synthesis on ScienceDirect