Methods included in this volume apply to the expression and characterization of retroviral proteases and their inhibitor/substrate design.


Biochemists, virologists, medicinal chemists, molecular biologists, immunologists, graduate students in these disciplines, and drug company researchers.

Table of Contents

Bacterial Expression and Enzymatic Assays: J. Stebbins and C. Debouck, Expression Systems for Retroviral Proteases. C.J. Rizzo and B.D. Korant, Genetic Approaches Designed to Minimize Cytotoxicity of Retroviral Protease. E. Chen, Host Strain Selection for Bacterial Expression of Toxic Proteins. C.U.T. Hellen, Assay Methods for Retroviral Proteases. A.H. Kaplan, M. Manchester, L. Everitt, and R. Swanstrom, Analysis of Human Immunodeficiency Virus 1 Protease Activity in Eukaryotic and Bacterial Cells. G. A. Krafft and G.T. Wang, Synthetic Approaches to Continuous Assays of Retroviral Proteases. Purification and Characterization: K. von der Helm, S. Seelmeier, A. Kisselev, and H.Nitschko, Identification, Purification, and Cell Culture Assays of Retroviral Proteases. P.L. Darke, Stability of Dimeric Retroviral Proteases. T.D. Meek, E.J. Rodriguez, and T.S. Angeles, Use of Steady State Kinetic Methods to Elucidate the Kinetic and Chemical Mechanisms of Retroviral Proteases. D. Ringe, X-Ray Structures of Retroviral Proteases and Their Inhibitor-Bound Complexes. W.E. Harte, Jr., and D.L. Beveridge, Probing Structure--Function Relationships in Human Immunodeficiency Virus 1 Protease via Molecular Dynamics Simulation. X.-L. Lin, Y.-Z. Lin, and J. Tang, Relationships of Human Immunodeficiency Virus Protease with Eukaryotic Aspartic Proteases. Substrate Specificity and Inhibitor Design: C. Carterand G. Zybarth, Processing of Retroviral Gag Polyproteins: An in Vitro Approach. B.M. Dunn, A. Gustchina, A. Wlodawer, and J. Kay, Subsite Preferences of Retroviral Proteinases. A.G


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© 1994
Academic Press
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