Bacterial Expression and Enzymatic Assays:
J. Stebbins and C. Debouck, Expression Systems for Retroviral Proteases.
C.J. Rizzo and B.D. Korant, Genetic Approaches Designed to Minimize Cytotoxicity of Retroviral Protease.
E. Chen, Host Strain Selection for Bacterial Expression of Toxic Proteins.
C.U.T. Hellen, Assay Methods for Retroviral Proteases.
A.H. Kaplan, M. Manchester, L. Everitt, and R. Swanstrom, Analysis of Human Immunodeficiency Virus 1 Protease Activity in Eukaryotic and Bacterial Cells.
G. A. Krafft and G.T. Wang, Synthetic Approaches to Continuous Assays of Retroviral Proteases.
Purification and Characterization:
K. von der Helm, S. Seelmeier, A. Kisselev, and H.Nitschko, Identification, Purification, and Cell Culture Assays of Retroviral Proteases.
P.L. Darke, Stability of Dimeric Retroviral Proteases.
T.D. Meek, E.J. Rodriguez, and T.S. Angeles, Use of Steady State Kinetic Methods to Elucidate the Kinetic and Chemical Mechanisms of Retroviral Proteases.
D. Ringe, X-Ray Structures of Retroviral Proteases and Their Inhibitor-Bound Complexes.
W.E. Harte, Jr., and D.L. Beveridge, Probing Structure--Function Relationships in Human Immunodeficiency Virus 1 Protease via Molecular Dynamics Simulation.
X.-L. Lin, Y.-Z. Lin, and J. Tang, Relationships of Human Immunodeficiency Virus Protease with Eukaryotic Aspartic Proteases.
Substrate Specificity and Inhibitor Design:
C. Carterand G. Zybarth, Processing of Retroviral Gag Polyproteins: An in Vitro Approach.
B.M. Dunn, A. Gustchina, A. Wlodawer, and J. Kay, Subsite Preferences of Retroviral Proteinases.
Methods included in this volume apply to the expression and characterization of retroviral proteases and their inhibitor/substrate design.
Biochemists, virologists, medicinal chemists, molecular biologists, immunologists, graduate students in these disciplines, and drug company researchers.
- No. of pages:
- © Academic Press 1994
- 22nd September 1994
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
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Merck Sharp & Dohme Research Laboratories, Merck & Co., West Point, Pennsylvania, U.S.A.
Merck Sharp & Dohme Research Laboratories, Merck & Co., Inc., West Point, Pennsylvania, U.S.A.
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA