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1. An Introduction and applications of CRISPR-Cas Systems
2. History, evolution and classification of CRISPR-Cas associated systems
Nisheeth Agrawal and Radhika Gupta
3. CRISPR based bacterial genome editing and removal of pathogens
4. CRISPR based genome editing and removal of human viruses
5. CRISPR based development of RNA editing and diagnostic platform
6. CRISPR based pathogenic fungus genome editing for control of infections
7. Genome engineering in insects for control of vector borne diseases
Stanislaus Antony Ceasar and V. Edwin Hillary
8. Development of insect cell line using CRISPR technology
Deepak Balaji Thimiri Govindaraj
9. CRISPRing protozoan parasites to better understand the biology of diseases
10. CRISPR based genome editing of Caenorhabditis elegans
11. CRISPR based genome editing of zebra fish
12. Using CRISPR-Cas9 based genome engineering tools in Drosophila melanogaster
13. CRISPR interference and its applications
14. CRISPR-Cas systems: Challenges and Future Prospects
Nisarg Gohil, Gargi Bhattacharjee and Vijai Singh
15. Patents and technology transfer in CRISPR technology
Deepak Balaji Thimiri Govindaraj
Reprogramming of the Genome: Applications of CRISPR-Cas in Non-mammalian Systems, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume covers several lower and model organisms that have been used for genome editing for creating a model for understanding of the molecular mechanism, disease model, removal of bacteria, virus, fungi, protozoans, insects, among others. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas.
- Offers basic understanding and a clear picture of genome editing CRISPR-Cas systems in different organisms
- Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for removal of virus, bacteria, fungi, protozoan and many more
- Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology and many more
Beginner in the area of genome engineering area, but also researchers, students, scientists, clinicians, stakeholders, policy makers and practitioners and many more
- No. of pages:
- © Academic Press 2021
- 1st April 2021
- Academic Press
- Hardcover ISBN:
Vijai Singh is an Associate Professor and Head of the Department of Biosciences, School of Science at Indrashil University, Mehsana, Gujarat, India. He was an Assistant Professor in the Department of Biological Sciences and Biotechnology at Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology (2009) from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India with a research focus on the development of molecular and immunoassays for diagnosis of Aeromonas hydrophila. He has extensive experience in Synthetic Biology including MAGE, small regulatory RNAs, pathway designing, CRISPR-Cas systems, and microfluidics. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 8 years of research and teaching experience in synthetic biology, metabolic engineering, microbiology, and industrial microbiology. He has published 76 articles, 31 chapters and 6 books. He serves as an associate editor, editorial board member and reviewer of a number of peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of Institutional Biosafety Committee (IBSC) in the same University.
Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, Gujarat, India
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