Reconstitutions of Transporters, Receptors, and Pathological States

Reconstitutions of Transporters, Receptors, and Pathological States

1st Edition - January 1, 1985

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  • Author: Efraim Racker
  • eBook ISBN: 9781483214238

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Description

Reconstitutions of Transporters, Receptors, and Pathological States presents lectures about the reconstitutions of transporters, receptors, and pathological states. The book discusses the principles and strategies of the resolution and reconstitution of soluble pathways and membrane complexes; and lessons in the resolution and reconstitution after the natural structure of the membrane has been destroyed. The text then describes the analyses of reconstituted vesicles; the ATP synthetase of oxidative phosphorylation; and the Ε1Ε2 pumps of plasma membranes. The ATP-driven ion pumps in organelles, microorganisms, and plants; the proton motive force generators, electron transport chains, and bacteriorhodopsin; and facilitated diffusion, symporters, and antiporters are also considered. The book further tackles plasma membrane receptors, as well as the reconstitutions of pathological states. Biochemists, molecular biologists, and cell biologists will find the book invaluable.

Table of Contents


  • Preface

    Lessons

    Abbreviations

    Lecture 1 Resolution and Reconstitution of Soluble Pathways and Membrane Complexes: Overview of Principles and Strategies

    I. Reconstitution of Soluble Pathways

    II. Resolution and Reconstitution of Membrane Complexes

    Lecture 2 Methods of Resolution and Reconstitution

    I. Solubilization and Purification of Membrane Proteins

    II. Purification of Membrane Proteins

    III. Methods of Reconstitution

    Lecture 3 What Can We Learn from Resolution and Reconstitution after the Natural Structure of the Membrane Has Been Destroyed

    I. What Are the Protein Components of the System and What Are Their Functions

    II. What Are the Phospholipid Components and What Are Their Functions

    III. What Is the Role of Asymmetry? How Do We Achieve It in Reconstitution? How Do We Measure It

    IV. How Do We Measure the Extent of Scrambling during Reconstitution

    VI Contents

    Lecture 4 Analyses of Reconstituted Vesicles: Pitfalls and Obstacles

    I. Analysis of Reconstituted Vesicles

    II. Pitfalls and Recommended Cautions

    Lecture 5 The ATP Synthetase of Oxidative Phosphorylation

    I. F1 (Mitochondrial MF1, Chloroplast CF1, and Bacterial BF1)

    II. The Stalk, OSCP, and F6

    III. The Hydrophobie Sector

    IV. Mechanism of Action of F1

    Lecture 6 The E1E2 Pumps of Plasma Membranes

    I. The Na+,K+ Pump and Na+,K+-ATPase

    II. The Ca2+ Pump and Ca2+-ATPase

    III. The H+,K+-ATPase of the Gastric Mucosa

    Lecture 7

    I. The Ca2+ Pump of Sarcoplasmic Reticulum and Related Organelles

    II. ATP-Driven Ion Pumps in Organelles, Microorganisms, and Plants

    III. The ATP-Driven H+ Fluxes in Organelles ATP-Driven Ion Pumps of Microorganisms and Plants

    Lecture 8 Proton Motive Force Generators, Electron Transport Chains, and Bacteriorhodopsin

    I. Reconstitution of the Mitochondrial Electron Transport Chain

    II. Reconstitution of Photosynthetic Electron Transport Pathways

    III. Bacteriorhodopsin

    IV. Halorhodopsin and a Bacterial Na+ Pump

    Lecture 9 Facilitation Diffusion, Symporters, and Antiporters

    I. Transporters of Plasma Membranes

    II. Transporters of Mitochondria

    III. Transporters of Other Organelles

    Lecture 10 Plasma Membrane Receptors

    I. RGC Receptors

    II. Polypeptide Signal Receptors

    III. Channel Receptors

    IV. Transport Receptors

    V. Drug and Toxin Receptors

    Lecture 11 Reconstitution of Pathological States

    I. About the Artificiality of Cancer Research

    II. Two Approaches to Cancer Research

    III. The Scenic Route from ABC to X

    Lecture 12 Glimpses into the Future of Reconstitutions (Hypotheses, Speculations, and Fantasies)

    I. Methods of Reconstitution

    II. Orientation-Directed Reconstitution and Co-reconstitutions

    III. Mechanisms and Regulations

    IV. Incorporations of Cellular Components into Cells

    V. Reconstitution of Organelles, Cells, Organs, etc

    VI. Reconstitution of Pathological States

    Bibliography

    Index


Product details

  • No. of pages: 288
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: January 1, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9781483214238

About the Author

Efraim Racker

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