
Reconstitutions of Transporters, Receptors, and Pathological States
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Reconstitutions of Transporters, Receptors, and Pathological States presents lectures about the reconstitutions of transporters, receptors, and pathological states. The book discusses the principles and strategies of the resolution and reconstitution of soluble pathways and membrane complexes; and lessons in the resolution and reconstitution after the natural structure of the membrane has been destroyed. The text then describes the analyses of reconstituted vesicles; the ATP synthetase of oxidative phosphorylation; and the Ε1Ε2 pumps of plasma membranes. The ATP-driven ion pumps in organelles, microorganisms, and plants; the proton motive force generators, electron transport chains, and bacteriorhodopsin; and facilitated diffusion, symporters, and antiporters are also considered. The book further tackles plasma membrane receptors, as well as the reconstitutions of pathological states. Biochemists, molecular biologists, and cell biologists will find the book invaluable.
Table of Contents
Preface
Lessons
Abbreviations
Lecture 1 Resolution and Reconstitution of Soluble Pathways and Membrane Complexes: Overview of Principles and Strategies
I. Reconstitution of Soluble Pathways
II. Resolution and Reconstitution of Membrane Complexes
Lecture 2 Methods of Resolution and Reconstitution
I. Solubilization and Purification of Membrane Proteins
II. Purification of Membrane Proteins
III. Methods of Reconstitution
Lecture 3 What Can We Learn from Resolution and Reconstitution after the Natural Structure of the Membrane Has Been Destroyed
I. What Are the Protein Components of the System and What Are Their Functions
II. What Are the Phospholipid Components and What Are Their Functions
III. What Is the Role of Asymmetry? How Do We Achieve It in Reconstitution? How Do We Measure It
IV. How Do We Measure the Extent of Scrambling during Reconstitution
VI Contents
Lecture 4 Analyses of Reconstituted Vesicles: Pitfalls and Obstacles
I. Analysis of Reconstituted Vesicles
II. Pitfalls and Recommended Cautions
Lecture 5 The ATP Synthetase of Oxidative Phosphorylation
I. F1 (Mitochondrial MF1, Chloroplast CF1, and Bacterial BF1)
II. The Stalk, OSCP, and F6
III. The Hydrophobie Sector
IV. Mechanism of Action of F1
Lecture 6 The E1E2 Pumps of Plasma Membranes
I. The Na+,K+ Pump and Na+,K+-ATPase
II. The Ca2+ Pump and Ca2+-ATPase
III. The H+,K+-ATPase of the Gastric Mucosa
Lecture 7
I. The Ca2+ Pump of Sarcoplasmic Reticulum and Related Organelles
II. ATP-Driven Ion Pumps in Organelles, Microorganisms, and Plants
III. The ATP-Driven H+ Fluxes in Organelles ATP-Driven Ion Pumps of Microorganisms and Plants
Lecture 8 Proton Motive Force Generators, Electron Transport Chains, and Bacteriorhodopsin
I. Reconstitution of the Mitochondrial Electron Transport Chain
II. Reconstitution of Photosynthetic Electron Transport Pathways
III. Bacteriorhodopsin
IV. Halorhodopsin and a Bacterial Na+ Pump
Lecture 9 Facilitation Diffusion, Symporters, and Antiporters
I. Transporters of Plasma Membranes
II. Transporters of Mitochondria
III. Transporters of Other Organelles
Lecture 10 Plasma Membrane Receptors
I. RGC Receptors
II. Polypeptide Signal Receptors
III. Channel Receptors
IV. Transport Receptors
V. Drug and Toxin Receptors
Lecture 11 Reconstitution of Pathological States
I. About the Artificiality of Cancer Research
II. Two Approaches to Cancer Research
III. The Scenic Route from ABC to X
Lecture 12 Glimpses into the Future of Reconstitutions (Hypotheses, Speculations, and Fantasies)
I. Methods of Reconstitution
II. Orientation-Directed Reconstitution and Co-reconstitutions
III. Mechanisms and Regulations
IV. Incorporations of Cellular Components into Cells
V. Reconstitution of Organelles, Cells, Organs, etc
VI. Reconstitution of Pathological States
Bibliography
Index
Product details
- No. of pages: 288
- Language: English
- Copyright: © Academic Press 1985
- Published: January 1, 1985
- Imprint: Academic Press
- eBook ISBN: 9781483214238
About the Author
Efraim Racker
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