Reconstitutions of Transporters, Receptors, and Pathological States

Reconstitutions of Transporters, Receptors, and Pathological States

1st Edition - January 28, 1985

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  • Author: Efraim Racker
  • eBook ISBN: 9780323157520

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Description

Reconstitutions of Transporters, Receptors, and Pathological States presents 12 lectures on the resolution and reconstitution of transporters, receptors, and pathological states. Lecture 1 discusses the reconstitution of soluble pathways, and the resolution and reconstitution of membrane complexes. Lecture 2 covers the solubilization and purification of membrane proteins. Lecture 3 explains the functions of protein and phospholipid components; the role of asymmetry; and measurement of scrambling during reconstitution. Lecture 4 presents analyses of reconstituted vesicles while Lectures 5 and 6 examine the properties of F1 and E1E2 pumps, respectively. Lecture 7 focuses on ATP-driven H+ fluxes in organelles and ATP-driven ion pumps of microorganisms and plants. Lecture 8 covers the reconstitution of the mitochondrial electron transport chain; reconstitution of photosynthetic electron transport pathways; and bacteriorhodopsin and halorhodopsin. Lecture 9 discusses the transporters of plasma membranes, mithchondria, and organelles. Lecture 10 deals with plasma membrane receptors. Lecture 11 focuses on the malignant transformation of cells while Lecture 12 speculates on the future of reconstitutions.

Table of Contents


  • Preface

    Lessons

    Abbreviations

    Lecture 1 Resolution and Reconstitution of Soluble Pathways and Membrane Complexes: Overview of Principles and Strategies

    I. Reconstitution of Soluble Pathways

    II. Resolution and Reconstitution of Membrane Complexes

    Lecture 2 Methods of Resolution and Reconstitution

    I. Solubilization and Purification of Membrane Proteins

    II. Purification of Membrane Proteins

    III. Methods of Reconstitution

    Lecture 3 What Can We Learn from Resolution and Reconstitution after the Natural Structure of the Membrane Has Been Destroyed?

    I. What Are the Protein Components of the System and What Are Their Functions?

    II. What Are the Phospholipid Components and What Are Their Functions?

    III. What Is the Role of Asymmetry? How Do We Achieve It in Reconstitution? How Do We Measure It?

    IV. How Do We Measure the Extent of Scrambling during Reconstitution?

    Lecture 4 Analyses of Reconstituted Vesicles: Pitfalls and Obstacles

    I. Analysis of Reconstituted Vesicles

    II. Pitfalls and Recommended Cautions

    Lecture 5 The ATP Synthetase of Oxidative Phosphorylation

    I. F1 (Mitochondrial MF1, Chloroplast CF1, and Bacterial BF1)

    II. The Stalk, OSCP, and F6

    III. The Hydrophobic Sector

    IV. Mechanism of Action of F1

    Lecture 6 The E1E2 Pumps of Plasma Membranes

    I. The Na+, K+ Pump and Na+,K+-ATPase

    II. The Ca2+ Pump and Ca2+-ATPase

    III. The H+,K+-ATPase of the Gastric Mucosa

    Lecture 7 ATP-Driven Ion Pumps in Organelles, Microorganisms, and Plants

    I. The Ca2+ Pump of Sarcoplasmic Reticulum and Related Organelles

    II. The ATP-Driven H+ Fluxes in Organelles

    III. ATP-Driven Ion Pumps of Microorganisms and Plants

    Lecture 8 Proton Motive Force Generators, Electron Transport Chains, and Bacteriorhodopsin

    I. Reconstitution of the Mitochondrial Electron Transport Chain

    II. Reconstitution of Photosynthetic Electron Transport Pathways

    III. Bacteriorhodopsin

    IV. Halorhodopsin and a Bacterial Na+ Pump

    Lecture 9 Facilitation Diffusion, Symporters, and Antiporters

    I. Transporters of Plasma Membranes

    II. Transporters of Mitochondria

    III. Transporters of Other Organelles

    Lecture 10 Plasma Membrane Receptors

    I. RGC Receptors

    II. Polypeptide Signal Receptors

    III. Channel Receptors

    IV. Transport Receptors

    V. Drug and Toxin Receptors

    Lecture 11 Reconstitution of Pathological States

    I. About the Artificiality of Cancer Research

    II. Two Approaches to Cancer Research

    III. The Scenic Route from ABC to X

    Lecture 12 Glimpses into the Future of Reconstitutions (Hypotheses, Speculations, and Fantasies)

    I. Methods of Reconstitution

    II. Orientation-Directed Reconstitution and Co-reconstitutions

    III. Mechanisms and Regulations

    IV. Incorporations of Cellular Components into Cells

    V. Reconstitution of Organelles, Cells, Organs, etc.

    VI. Reconstitution of Pathological States

    Bibliography

    Index


Product details

  • No. of pages: 288
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: January 28, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9780323157520

About the Author

Efraim Racker

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