Reconstitutions of Transporters, Receptors, and Pathological States

Reconstitutions of Transporters, Receptors, and Pathological States presents 12 lectures on the resolution and reconstitution of transporters, receptors, and pathological states. Lecture 1 discusses the reconstitution of soluble pathways, and the resolution and reconstitution of membrane complexes. Lecture 2 covers the solubilization and purification of membrane proteins. Lecture 3 explains the functions of protein and phospholipid components; the role of asymmetry; and measurement of scrambling during reconstitution. Lecture 4 presents analyses of reconstituted vesicles while Lectures 5 and 6 examine the properties of F1 and E1E2 pumps, respectively. Lecture 7 focuses on ATP-driven H+ fluxes in organelles and ATP-driven ion pumps of microorganisms and plants. Lecture 8 covers the reconstitution of the mitochondrial electron transport chain; reconstitution of photosynthetic electron transport pathways; and bacteriorhodopsin and halorhodopsin. Lecture 9 discusses the transporters of plasma membranes, mithchondria, and organelles. Lecture 10 deals with plasma membrane receptors. Lecture 11 focuses on the malignant transformation of cells while Lecture 12 speculates on the future of reconstitutions.

Preface

Lessons

Abbreviations

Lecture 1 Resolution and Reconstitution of Soluble Pathways and Membrane Complexes: Overview of Principles and Strategies

I. Reconstitution of Soluble Pathways

II. Resolution and Reconstitution of Membrane Complexes

Lecture 2 Methods of Resolution and Reconstitution

I. Solubilization and Purification of Membrane Proteins

II. Purification of Membrane Proteins

III. Methods of Reconstitution

Lecture 3 What Can We Learn from Resolution and Reconstitution after the Natural Structure of the Membrane Has Been Destroyed?

I. What Are the Protein Components of the System and What Are Their Functions?

II. What Are the Phospholipid Components and What Are Their Functions?

III. What Is the Role of Asymmetry? How Do We Achieve It in Reconstitution? How Do We Measure It?

IV. How Do We Measure the Extent of Scrambling during Reconstitution?

Lecture 4 Analyses of Reconstituted Vesicles: Pitfalls and Obstacles

I. Analysis of Reconstituted Vesicles

II. Pitfalls and Recommended Cautions

Lecture 5 The ATP Synthetase of Oxidative Phosphorylation

I. F1 (Mitochondrial MF1, Chloroplast CF1, and Bacterial BF1)

II. The Stalk, OSCP, and F6

III. The Hydrophobic Sector

IV. Mechanism of Action of F1

Lecture 6 The E1E2 Pumps of Plasma Membranes

I. The Na+, K+ Pump and Na+,K+-ATPase

II. The Ca2+ Pump and Ca2+-ATPase

III. The H+,K+-ATPase of the Gastric Mucosa

Lecture 7 ATP-Driven Ion Pumps in Organelles, Microorganisms, and Plants

I. The Ca2+ Pump of Sarcoplasmic Reticulum and Related Organelles

II. The ATP-Driven H+ Fluxes in Organelles

III. ATP-Driven Ion Pumps of Microorganisms and Plants

Lecture 8 Proton Motive Force Generators, Electron Transport Chains, and Bacteriorhodopsin

I. Reconstitution of the Mitochondrial Electron Transport Chain

II. Reconstitution of Photosynthetic Electron Transport Pathways

III. Bacteriorhodopsin

IV. Halorhodopsin and a Bacterial Na+ Pump

Lecture 9 Facilitation Diffusion, Symporters, and Antiporters

I. Transporters of Plasma Membranes

II. Transporters of Mitochondria

III. Transporters of Other Organelles

Lecture 10 Plasma Membrane Receptors

I. RGC Receptors

II. Polypeptide Signal Receptors

III. Channel Receptors

IV. Transport Receptors

V. Drug and Toxin Receptors

Lecture 11 Reconstitution of Pathological States

I. About the Artificiality of Cancer Research

II. Two Approaches to Cancer Research

III. The Scenic Route from ABC to X

Lecture 12 Glimpses into the Future of Reconstitutions (Hypotheses, Speculations, and Fantasies)

I. Methods of Reconstitution

II. Orientation-Directed Reconstitution and Co-reconstitutions

III. Mechanisms and Regulations

IV. Incorporations of Cellular Components into Cells

V. Reconstitution of Organelles, Cells, Organs, etc.

VI. Reconstitution of Pathological States

Bibliography

Index