Reconstitutions of Transporters, Receptors, and Pathological States

PrefaceLessonsAbbreviationsLecture 1 Resolution and Reconstitution of Soluble Pathways and Membrane Complexes: Overview of Principles and Strategies I. Reconstitution of Soluble Pathways II. Resolution and Reconstitution of Membrane ComplexesLecture 2 Methods of Resolution and Reconstitution I. Solubilization and Purification of Membrane Proteins II. Purification of Membrane Proteins III. Methods of ReconstitutionLecture 3 What Can We Learn from Resolution and Reconstitution after the Natural Structure of the Membrane Has Been Destroyed? I. What Are the Protein Components of the System and What Are Their Functions? II. What Are the Phospholipid Components and What Are Their Functions? III. What Is the Role of Asymmetry? How Do We Achieve It in Reconstitution? How Do We Measure It? IV. How Do We Measure the Extent of Scrambling during Reconstitution?Lecture 4 Analyses of Reconstituted Vesicles: Pitfalls and Obstacles I. Analysis of Reconstituted Vesicles II. Pitfalls and Recommended CautionsLecture 5 The ATP Synthetase of Oxidative Phosphorylation I. F1 (Mitochondrial MF1, Chloroplast CF1, and Bacterial BF1) II. The Stalk, OSCP, and F6 III. The Hydrophobic Sector IV. Mechanism of Action of F1Lecture 6 The E1E2 Pumps of Plasma Membranes I. The Na+, K+ Pump and Na+,K+-ATPase II. The Ca2+ Pump and Ca2+-ATPase III. The H+,K+-ATPase of the Gastric MucosaLecture 7 ATP-Driven Ion Pumps in Organelles, Microorganisms, and Plants I. The Ca2+ Pump of Sarcoplasmic Reticulum and Related Organelles II. The ATP-Driven H+ Fluxes in Organelles III. ATP-Driven Ion Pumps of Microorganisms and PlantsLecture 8 Proton Motive Force Generators, Electron Transport Chains, and Bacteriorhodopsin I. Reconstitution of the Mitochondrial Electron Transport Chain II. Reconstitution of Photosynthetic Electron Transport Pathways III. Bacteriorhodopsin IV. Halorhodopsin and a Bacterial Na+ PumpLecture 9 Facilitation Diffusion, Symporters, and Antiporters I. Transporters of Plasma Membranes II. Transporters of Mitochondria III. Transporters of Other OrganellesLecture 10 Plasma Membrane Receptors I. RGC Receptors II. Polypeptide Signal Receptors III. Channel Receptors IV. Transport Receptors V. Drug and Toxin ReceptorsLecture 11 Reconstitution of Pathological States I. About the Artificiality of Cancer Research II. Two Approaches to Cancer Research III. The Scenic Route from ABC to XLecture 12 Glimpses into the Future of Reconstitutions (Hypotheses, Speculations, and Fantasies) I. Methods of Reconstitution II. Orientation-Directed Reconstitution and Co-reconstitutions III. Mechanisms and Regulations IV. Incorporations of Cellular Components into Cells V. Reconstitution of Organelles, Cells, Organs, etc. VI. Reconstitution of Pathological StatesBibliographyIndex