Reconstitutions of Transporters, Receptors, and Pathological States

Reconstitutions of Transporters, Receptors, and Pathological States presents 12 lectures on the resolution and reconstitution of transporters, receptors, and pathological states. Lecture 1 discusses the reconstitution of soluble pathways, and the resolution and reconstitution of membrane complexes. Lecture 2 covers the solubilization and purification of membrane proteins. Lecture 3 explains the functions of protein and phospholipid components; the role of asymmetry; and measurement of scrambling during reconstitution. Lecture 4 presents analyses of reconstituted vesicles while Lectures 5 and 6 examine the properties of F1 and E1E2 pumps, respectively. Lecture 7 focuses on ATP-driven H+ fluxes in organelles and ATP-driven ion pumps of microorganisms and plants. Lecture 8 covers the reconstitution of the mitochondrial electron transport chain; reconstitution of photosynthetic electron transport pathways; and bacteriorhodopsin and halorhodopsin. Lecture 9 discusses the transporters of plasma membranes, mithchondria, and organelles. Lecture 10 deals with plasma membrane receptors. Lecture 11 focuses on the malignant transformation of cells while Lecture 12 speculates on the future of reconstitutions.

Preface Lessons Abbreviations Lecture 1 Resolution and Reconstitution of Soluble Pathways and Membrane Complexes: Overview of Principles and Strategies I. Reconstitution of Soluble Pathways II. Resolution and Reconstitution of Membrane Complexes Lecture 2 Methods of Resolution and Reconstitution I. Solubilization and Purification of Membrane Proteins II. Purification of Membrane Proteins III. Methods of Reconstitution Lecture 3 What Can We Learn from Resolution and Reconstitution after the Natural Structure of the Membrane Has Been Destroyed? I. What Are the Protein Components of the System and What Are Their Functions? II. What Are the Phospholipid Components and What Are Their Functions? III. What Is the Role of Asymmetry? How Do We Achieve It in Reconstitution? How Do We Measure It? IV. How Do We Measure the Extent of Scrambling during Reconstitution? Lecture 4 Analyses of Reconstituted Vesicles: Pitfalls and Obstacles I. Analysis of Reconstituted Vesicles II. Pitfalls and Recommended Cautions Lecture 5 The ATP Synthetase of Oxidative Phosphorylation I. F1 (Mitochondrial MF1, Chloroplast CF1, and Bacterial BF1) II. The Stalk, OSCP, and F6 III. The Hydrophobic Sector IV. Mechanism of Action of F1 Lecture 6 The E1E2 Pumps of Plasma Membranes I. The Na+, K+ Pump and Na+,K+-ATPase II. The Ca2+ Pump and Ca2+-ATPase III. The H+,K+-ATPase of the Gastric Mucosa Lecture 7 ATP-Driven Ion Pumps in Organelles, Microorganisms, and Plants I. The Ca2+ Pump of Sarcoplasmic Reticulum and Related Organelles II. The ATP-Driven H+ Fluxes in Organelles III. ATP-Driven Ion Pumps of Microorganisms and Plants Lecture 8 Proton Motive Force Generators, Electron Transport Chains, and Bacteriorhodopsin I. Reconstitution of the Mitochondrial Electron Transport Chain II. Reconstitution of Photosynthetic Electron Transport Pathways III. Bacte