
Reconstitutions of Transporters, Receptors, and Pathological States
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Reconstitutions of Transporters, Receptors, and Pathological States presents 12 lectures on the resolution and reconstitution of transporters, receptors, and pathological states. Lecture 1 discusses the reconstitution of soluble pathways, and the resolution and reconstitution of membrane complexes. Lecture 2 covers the solubilization and purification of membrane proteins. Lecture 3 explains the functions of protein and phospholipid components; the role of asymmetry; and measurement of scrambling during reconstitution. Lecture 4 presents analyses of reconstituted vesicles while Lectures 5 and 6 examine the properties of F1 and E1E2 pumps, respectively. Lecture 7 focuses on ATP-driven H+ fluxes in organelles and ATP-driven ion pumps of microorganisms and plants. Lecture 8 covers the reconstitution of the mitochondrial electron transport chain; reconstitution of photosynthetic electron transport pathways; and bacteriorhodopsin and halorhodopsin. Lecture 9 discusses the transporters of plasma membranes, mithchondria, and organelles. Lecture 10 deals with plasma membrane receptors. Lecture 11 focuses on the malignant transformation of cells while Lecture 12 speculates on the future of reconstitutions.
Table of Contents
Preface
Lessons
Abbreviations
Lecture 1 Resolution and Reconstitution of Soluble Pathways and Membrane Complexes: Overview of Principles and Strategies
I. Reconstitution of Soluble Pathways
II. Resolution and Reconstitution of Membrane Complexes
Lecture 2 Methods of Resolution and Reconstitution
I. Solubilization and Purification of Membrane Proteins
II. Purification of Membrane Proteins
III. Methods of Reconstitution
Lecture 3 What Can We Learn from Resolution and Reconstitution after the Natural Structure of the Membrane Has Been Destroyed?
I. What Are the Protein Components of the System and What Are Their Functions?
II. What Are the Phospholipid Components and What Are Their Functions?
III. What Is the Role of Asymmetry? How Do We Achieve It in Reconstitution? How Do We Measure It?
IV. How Do We Measure the Extent of Scrambling during Reconstitution?
Lecture 4 Analyses of Reconstituted Vesicles: Pitfalls and Obstacles
I. Analysis of Reconstituted Vesicles
II. Pitfalls and Recommended Cautions
Lecture 5 The ATP Synthetase of Oxidative Phosphorylation
I. F1 (Mitochondrial MF1, Chloroplast CF1, and Bacterial BF1)
II. The Stalk, OSCP, and F6
III. The Hydrophobic Sector
IV. Mechanism of Action of F1
Lecture 6 The E1E2 Pumps of Plasma Membranes
I. The Na+, K+ Pump and Na+,K+-ATPase
II. The Ca2+ Pump and Ca2+-ATPase
III. The H+,K+-ATPase of the Gastric Mucosa
Lecture 7 ATP-Driven Ion Pumps in Organelles, Microorganisms, and Plants
I. The Ca2+ Pump of Sarcoplasmic Reticulum and Related Organelles
II. The ATP-Driven H+ Fluxes in Organelles
III. ATP-Driven Ion Pumps of Microorganisms and Plants
Lecture 8 Proton Motive Force Generators, Electron Transport Chains, and Bacteriorhodopsin
I. Reconstitution of the Mitochondrial Electron Transport Chain
II. Reconstitution of Photosynthetic Electron Transport Pathways
III. Bacteriorhodopsin
IV. Halorhodopsin and a Bacterial Na+ Pump
Lecture 9 Facilitation Diffusion, Symporters, and Antiporters
I. Transporters of Plasma Membranes
II. Transporters of Mitochondria
III. Transporters of Other Organelles
Lecture 10 Plasma Membrane Receptors
I. RGC Receptors
II. Polypeptide Signal Receptors
III. Channel Receptors
IV. Transport Receptors
V. Drug and Toxin Receptors
Lecture 11 Reconstitution of Pathological States
I. About the Artificiality of Cancer Research
II. Two Approaches to Cancer Research
III. The Scenic Route from ABC to X
Lecture 12 Glimpses into the Future of Reconstitutions (Hypotheses, Speculations, and Fantasies)
I. Methods of Reconstitution
II. Orientation-Directed Reconstitution and Co-reconstitutions
III. Mechanisms and Regulations
IV. Incorporations of Cellular Components into Cells
V. Reconstitution of Organelles, Cells, Organs, etc.
VI. Reconstitution of Pathological States
Bibliography
Index
Product details
- No. of pages: 288
- Language: English
- Copyright: © Academic Press 1985
- Published: January 28, 1985
- Imprint: Academic Press
- eBook ISBN: 9780323157520
About the Author
Efraim Racker
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