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Reconstitution of Intracellular Transport - 1st Edition - ISBN: 9780121821203, 9780080883328

Reconstitution of Intracellular Transport, Volume 219

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: James Rothman
eBook ISBN: 9780080883328
Imprint: Academic Press
Published Date: 23rd November 1992
Page Count: 438


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Table of Contents

J.E. Rothman, Introduction. Reconstitution in Cell-Free Extracts: C.J.M. Beckers and J.E. Rothman, Transport between Golgi Cisternae. W.A. Braell, Detection of Endocytic Vesicle Fusion in Vitro Using an Assay Based on the Avidin-Biotin Association Reaction. A. Pitt, L.S. Mayorga, A.L. Schwartz, and P. Stahl, Assays for Phagosome-Endosome Fusion and Phagosome Protein Recycling. M.I. Colombo, J.M. Lenhard, L.S. Mayorga, and P. Stahl, Reconstitution of Endosome Fusion: Identification of Factors Necessary for Fusion Competency. E. Sztul, Transcytotic Vesicle Fusion with the Plasma Membrane. B.M. Mullock and J.P. Luzio, Reconstitution of Rat Liver Endosome-Lysosome Fusion in Vitro. R. Pfaller and J.W. Newport, Nuclear Envelope Assembly following Mitosis. J.M. McIlvain and M. Sheetz, Formation of ER Networks in Vitro. S. Tooze and W. Huttner, Cell-Free Formation of Immature Secretory Granules and Constitutive Secretory Vesicles from the Trans-Golgi Network. Reconstitution Using Semi-intact and Perforated Cells: S.A. Adam, R. Sterne-Marr, and L. Gerace, Analysis of Nuclear Protein Import Using Digitonin-Permeabilized Cells. R. Schwaninger, H. Plutner, H. Davidson, S. Pind, and W. Balch, Transport of Protein between Endoplasmic Reticulum and Golgi Compartments in Semi-intact Cells. L.J. Wuestehube and R. Schekman, Reconstitution of Transport from Endoplasmic Reticulum to Golgi Complex Using an ER-Enriched Membrane Fraction from Yeast. M.E. Groesch, G. Rossi, and S. Ferro-Novick, Reconstitution of ER to Golgi Transport in Yeast: Use of an in Vitro Assay to Characterize Secretory Mutants and Functional Transport Vesicles. Y. Goda, T. Soldati, and S.R. Pfeffer, Transport from Late Endosomes to Trans Golgi Network in Semi-intact Cell Extracts. I. Corthesy-Theulaz and S.R. Pfeffer, Microtubule-Mediated Golgi Capture by Semi-intact CHO Cells. R.W. Holz, M.A. Bittner, and R.A. Senter, Regulated Exocytotic Fusion I: Chromaffin Cells and PC12 Cells. B.D. Gomperts and P.E.R. Tatham, Regulated Exocytotic Secretion from Permeabilized Cells. F. Wieland, Transfer of Bulk Markers from ER to Plasma Membrane. B. Podbiliewicz and I. Mellman, Reconstitution of Endocytosis and Recycling Using Perforated MDCK Cells. M. Pypaert and G. Warren, Morphological Studies of Formation of Coated Pits and Coated Vesicles in Broken Cells. E. Smythe, T.E. Redelmeier, and S.L. Schmid, Receptor-Mediated Endocytosis in Semi-intact Cells. S.G. Miller and H.H. Moore, Movement from Trans-Golgi Network to Cell Surface in Semi-intact Cells. Identification of Transport Intermediates: P.G. Woodman and G. Warren, Isolation and Characterization of Functional, Clathrin Coated, Endocytic Vesicles. H.W. Davidson and W.E. Balch, Use of Two-Stage Incubations to Define Sequential Intermediates in ER to Golgi Transport. M.F. Rexach and R. Schekman, Use of sec Mutants to Define Intermediates in Protein Transport from ER. T. Serafini and J.E. Rothman, Purification of Golgi Cisternae-Derived COP-Coated Vesicles. M.R. Block and J.E. Rothman, Purification of an N-Ethylmaleimide-Sensitive Fusion Protein (NSF). D.W. Wilson and J.E. Rothman, Expression and Purification of Recombinant NSF from Escherichia coli. D.O. Clary and J.E. Rothman, Purification of SNAPs from Bovine Brain Microsomes. M.G. Waters, C.J.M. Beckers, and J.E. Rothman, Purification of Coat Protomers. L. Hicke, T. Yoshihisa, and R. Schekman, Purification of Yeast Sec23 Protein by Complementation of Mutant Cell Lysates Deficient in ER to Golgi Transport. P. Novick, M. Garrett, P. Brennwald, and A.K. Kabcenell, Purification of Sec4 Protein from S. cerevisiae and Escherichia coli. P.A. Randazzo, O. Weiss, and R.A. Kahn, Preparation of Recombinant ADP-Ribosylation Factor (ARF). P. Wagner, L. Hengst, and D. Gallwitz, Ypt Proteins in Yeast. A. Tavitian and A. Zahraoui, Rab Proteins and Gene Family in Animals. M. Zerial, R. Parton, P. Chavrier, and R. Frank, Localization of Rab Family Members in Animal Cells. Each chapter includes references. Author Index. Subject Index.


This volume includes sections on reconstitution in cell-free extracts, reconstitution using semi-intact and perforated cells, and identification of transport intermediates.


Biochemists, biophysicists, physical chemists, and molecular and cell biologists.


No. of pages:
© Academic Press 1992
23rd November 1992
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

James Rothman

Affiliations and Expertise

Sloan-Kettering Institute for Cancer Research, New York, U.S.A.