Reconstitution of Intracellular Transport, Volume 219

1st Edition

Print ISBN: 9780121821203
eBook ISBN: 9780080883328
Imprint: Academic Press
Published Date: 23rd November 1992
Page Count: 438


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This volume includes sections on reconstitution in cell-free extracts, reconstitution using semi-intact and perforated cells, and identification of transport intermediates.


Biochemists, biophysicists, physical chemists, and molecular and cell biologists.

Table of Contents

J.E. Rothman, Introduction.

Reconstitution in Cell-Free Extracts:

C.J.M. Beckers and J.E. Rothman, Transport between Golgi Cisternae.

W.A. Braell, Detection of Endocytic Vesicle Fusion in Vitro Using an Assay Based on the Avidin-Biotin Association Reaction.

A. Pitt, L.S. Mayorga, A.L. Schwartz, and P. Stahl, Assays for Phagosome-Endosome Fusion and Phagosome Protein Recycling.

M.I. Colombo, J.M. Lenhard, L.S. Mayorga, and P. Stahl, Reconstitution of Endosome Fusion: Identification of Factors Necessary for Fusion Competency.

E. Sztul, Transcytotic Vesicle Fusion with the Plasma Membrane.

B.M. Mullock and J.P. Luzio, Reconstitution of Rat Liver Endosome-Lysosome Fusion in Vitro.

R. Pfaller and J.W. Newport, Nuclear Envelope Assembly following Mitosis.

J.M. McIlvain and M. Sheetz, Formation of ER Networks in Vitro.

S. Tooze and W. Huttner, Cell-Free Formation of Immature Secretory Granules and Constitutive Secretory Vesicles from the Trans-Golgi Network.

Reconstitution Using Semi-intact and Perforated Cells:

S.A. Adam, R. Sterne-Marr, and L. Gerace, Analysis of Nuclear Protein Import Using Digitonin-Permeabilized Cells.

R. Schwaninger, H. Plutner, H. Davidson, S. Pind, and W. Balch, Transport of Protein between Endoplasmic Reticulum and Golgi Compartments in Semi-intact Cells.

L.J. Wuestehube and R. Schekman, Reconstitution of Transport from Endoplasmic Reticulum to Golgi Complex Using an ER-Enriched Membrane Fraction from Yeast.

M.E. Groesch, G. Rossi, and S. Ferro-Novick, Reconstitution of ER to Golgi Transport in Yeast: Use of an in Vitro Assay to Characterize Secretory Mutants and


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