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Recombinant Protein Expression: Eukaryotic hosts - 1st Edition - ISBN: 9780323907378

Recombinant Protein Expression: Eukaryotic hosts, Volume 660

1st Edition

Serial Volume Editors: Zvi Kelman William O'Dell
Hardcover ISBN: 9780323907378
Imprint: Academic Press
Published Date: 10th November 2021
Page Count: 380
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Table of Contents


William B. O’Dell and Zvi Kelman

1. Expression of recombinant multi-protein complexes in Saccharomyces cerevisiae

Ryan Mayle and Mike O’Donnell

2. Recombinant production of membrane proteins in yeast

Sarah Spruce Preisler, Karen Molbæk, Casper Normann Nurup, Mads Beich-Frandsen and Per Amstrup Pedersen

3. Expression of proteins in Pichia pastoris

Giuliana Mastropietro, Rochelle Aw and Karen M. Polizzi

4. Hybrid-architectured promoter design to engineer expression in yeast

Burcu Gündüz Ergün and Pinar Çalik

5. Hybrid-architectured promoter design to deregulate expression in yeast

Burcu Gündüz Ergün and Pinar Çalik

Baculovirus–insect cell systems

6. The MultiBac BEVS: Basics, Applications, Performance and Recent Developments

Barbara Gorda, Christine Toelzer, Francesco Aulicino and Imre Berger

7. Applications of Golden Gate Cloning to protein production using the baculovirus expression vector system

Erich Spielvogel, Jana Neuhold and Peggy Stolt-Bergner

8. A fast-track protocol for protein expression using the BEV system

Judith Scholz and Sabine Suppmann

Plant hosts

9. Transient expression of recombinant proteins in plants

S. Nosaki and K. Miura

10. Transient expression of recombinant proteins in plants using potato virus X based vectors

Eugenia S. Mardanova and Nikolai V. Ravin

11. Design and expression of a bispecific antibody against dengue and chikungunya virus in plants

Adrian Esqueda, Collin Jugler and Qiang Chen

12. Manufacturing plant-made monoclonal antibodies for research or therapeutic applications

Kelsi Swope, Josh Morton, Gergory P. Pogue, Steve Hume, Michael H. Pauly, John Shepherd, Carrie A. Simpson, Barry Bratcher, Kevin J. Whaley, Larry Zeitlin, Keith R. Davis and Hugh Haydon

Mammalian cell lines as biomanufacturing platforms

13. Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation

Douglas Nmagu, Sumit K. Singh and Kelvin H. Lee

14. Method to transfer Chinese hamster ovary (CHO) batch shake flask experiments to large-scale, computer-controlled fed-batch bioreactors

Stephanie R. Klaubert, Dylan G. Chitwood, Hussain Dahodwala, Madison Williamson, Rachel Kasper, Kelvin H. Lee and Sarah W. Harcum

15. Inducible protein expression in piggyBac transposase mediated stable HEK293 cell pools

Sabine Suppmann

16. Exploring the design space of AAV transient-transfection in suspension cell lines

Oliver Meade, Jeffery Clark, Michael McCutchen and John Kerwin


Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.

Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology serial
  • Updated release includes the latest information on Recombinant Protein Expression


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


No. of pages:
© Academic Press 2021
10th November 2021
Academic Press
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editors

Zvi Kelman

Zvi Kelman

Zvi Kelman is the Director of the Biomolecular Labeling Laboratory (BL2), National Institute of Standards and Technology (NIST). He is also an Adjunct Professor in the Department of Cell Biology and Molecular Genetics at the University of Maryland, College Park and affiliated with the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine. Zvi earned a B.Sc. in Agriculture from the Hebrew University in Jerusalem and a M.Sc. in Cell Biology from the Weizmann Institute of Science. After receiving a Ph.D. in Molecular Biology from Cornell University, he was a Helen Hay Whitney Foundation Post-Doctoral Fellow at Johns Hopkins University and Memorial Sloan-Kettering Cancer Center. Upon completion of his postgraduate training, he became a Life Technologies Professor at the Center for Advanced Research in Biotechnology (CARB), University of Maryland Biotechnology Institute (UMBI). Zvi moved to the University of Maryland, College Park in 2010 as a Professor in the Department of Cell Biology and Molecular Genetics. In 2011 he was recruited to NIST to direct the Biomolecular Labeling Laboratory.

Affiliations and Expertise

Director, Biomolecular Labeling Laboratory, NIST-IBBR, Rockville, MD, USA

William O'Dell

William Brad O'Dell is a PhD biologist experienced in in Molecular Biology, Protein Expression, Protein Purification, Crystallography, Lab-Scale Yeast Fermentation, Flow Cytometry and Small Angle Scattering of Molecules and Materials.

Affiliations and Expertise

Biologist, National Institute of Standards and Technology (NIST), MD, USA