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William B. O’Dell and Zvi Kelman
1. Expression of recombinant multi-protein complexes in Saccharomyces cerevisiae
Ryan Mayle and Mike O’Donnell
2. Recombinant production of membrane proteins in yeast
Sarah Spruce Preisler, Karen Molbæk, Casper Normann Nurup, Mads Beich-Frandsen and Per Amstrup Pedersen
3. Expression of proteins in Pichia pastoris
Giuliana Mastropietro, Rochelle Aw and Karen M. Polizzi
4. Hybrid-architectured promoter design to engineer expression in yeast
Burcu Gündüz Ergün and Pinar Çalik
5. Hybrid-architectured promoter design to deregulate expression in yeast
Burcu Gündüz Ergün and Pinar Çalik
Baculovirus–insect cell systems
6. The MultiBac BEVS: Basics, Applications, Performance and Recent Developments
Barbara Gorda, Christine Toelzer, Francesco Aulicino and Imre Berger
7. Applications of Golden Gate Cloning to protein production using the baculovirus expression vector system
Erich Spielvogel, Jana Neuhold and Peggy Stolt-Bergner
8. A fast-track protocol for protein expression using the BEV system
Judith Scholz and Sabine Suppmann
9. Transient expression of recombinant proteins in plants
S. Nosaki and K. Miura
10. Transient expression of recombinant proteins in plants using potato virus X based vectors
Eugenia S. Mardanova and Nikolai V. Ravin
11. Design and expression of a bispecific antibody against dengue and chikungunya virus in plants
Adrian Esqueda, Collin Jugler and Qiang Chen
12. Manufacturing plant-made monoclonal antibodies for research or therapeutic applications
Kelsi Swope, Josh Morton, Gergory P. Pogue, Steve Hume, Michael H. Pauly, John Shepherd, Carrie A. Simpson, Barry Bratcher, Kevin J. Whaley, Larry Zeitlin, Keith R. Davis and Hugh Haydon
Mammalian cell lines as biomanufacturing platforms
13. Creation of monoclonal antibody expressing CHO cell lines grown with sodium butyrate and characterization of resulting antibody glycosylation
Douglas Nmagu, Sumit K. Singh and Kelvin H. Lee
14. Method to transfer Chinese hamster ovary (CHO) batch shake flask experiments to large-scale, computer-controlled fed-batch bioreactors
Stephanie R. Klaubert, Dylan G. Chitwood, Hussain Dahodwala, Madison Williamson, Rachel Kasper, Kelvin H. Lee and Sarah W. Harcum
15. Inducible protein expression in piggyBac transposase mediated stable HEK293 cell pools
16. Exploring the design space of AAV transient-transfection in suspension cell lines
Oliver Meade, Jeffery Clark, Michael McCutchen and John Kerwin
Recombinant Protein Expression, Part B, Volume 660 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on Multiplexed analysis protein: Protein interactions of polypeptides translated in Leishmania cell-free system, MultiBac system and its applications, performance and recent, Production of antibodies in Shuffle, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to enhance transcription in yeast, Designing hybrid-promoter architectures by engineering cis-acting DNA sites to deregulate transcription in yeast, Antibody or protein-based vaccine production in plants, Cell-free protein synthesis, Plant-based expression of biologic drugs, and much more.
Additional sections cover the Use of native mass spectrometry to guide detergent-based rescue of non-native oligomerization by recombinant proteins, Advancing overexpression and purification of recombinant proteins by pilot optimization through tandem affinity-buffer exchange chromatography online with native mass spectrometry, Method for High-Efficiency Fed-batch cultures of recombinant Escherichia coli, Method to transfer Chinese hamster ovary (CHO) shake flask experiments to the ambr® 250, and Expression of recombinant antibodies in Leishmania tarentolae.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology serial
- Updated release includes the latest information on Recombinant Protein Expression
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- No. of pages:
- © Academic Press 2021
- 10th November 2021
- Academic Press
- Hardcover ISBN:
Zvi Kelman is the Director of the Biomolecular Labeling Laboratory (BL2), National Institute of Standards and Technology (NIST). He is also an Adjunct Professor in the Department of Cell Biology and Molecular Genetics at the University of Maryland, College Park and affiliated with the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine. Zvi earned a B.Sc. in Agriculture from the Hebrew University in Jerusalem and a M.Sc. in Cell Biology from the Weizmann Institute of Science. After receiving a Ph.D. in Molecular Biology from Cornell University, he was a Helen Hay Whitney Foundation Post-Doctoral Fellow at Johns Hopkins University and Memorial Sloan-Kettering Cancer Center. Upon completion of his postgraduate training, he became a Life Technologies Professor at the Center for Advanced Research in Biotechnology (CARB), University of Maryland Biotechnology Institute (UMBI). Zvi moved to the University of Maryland, College Park in 2010 as a Professor in the Department of Cell Biology and Molecular Genetics. In 2011 he was recruited to NIST to direct the Biomolecular Labeling Laboratory.
Director, Biomolecular Labeling Laboratory, NIST-IBBR, Rockville, MD, USA
William Brad O'Dell is a PhD biologist experienced in in Molecular Biology, Protein Expression, Protein Purification, Crystallography, Lab-Scale Yeast Fermentation, Flow Cytometry and Small Angle Scattering of Molecules and Materials.
Biologist, National Institute of Standards and Technology (NIST), MD, USA
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