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Recombinant DNA, Part I - 1st Edition - ISBN: 9780121821197, 9780080883311

Recombinant DNA, Part I, Volume 218

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: Ray Wu
eBook ISBN: 9780080883311
Imprint: Academic Press
Published Date: 11th May 1993
Page Count: 806


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Table of Contents

Methods for Sequencing DNA: U.B. Gyllensten and M. Allen, Methods for Sequencing of in Vitro Amplified DNA. L.F. Landweber and M. Kreitman, Strategy for Producing Single-Stranded DNA in Polymerase Chain Reaction for Direct Genomic Sequencing. J.G. Lawrence, D.L. Hartl, and H. Ochman, Sequencing Products of Polymerase Chain Reaction. R.L. Dorit, One-Sided PCR for Amplification and Sequencing cDNA. Z. Liu and P.B. Hackett, Reverse Cloning Procedure for Rapid Generation of Subclones for DNA Sequencing. W. Shen and M.M.Y. Waye, Simple Method for Generating Unidirectional Deletion Mutants for DNA Sequencing and Protein Engineering. K. Kretz and J.S. O'Brien, Direct Sequencing of PCR Products from Low-Melting Temperature Agarose. D.B. Olsen, G. Wunderlich, A. Uy, and F. Eckstein, Direct Sequencing of Polymerase Chain Reaction Products. J.P. Schofield, D.S.C. Jones, and M. Vaudin, Fluorescent and Radioactive Solid-Phase Dideoxy Sequencing of PCR Products in Microtiter Plates. Z. Du, L. Hood, and R.K. Wilson, Automated Fluorescent DNA Sequencing of PCR Products. R. Kaiser, T. Hunkapiller, C. Heiner, and L. Hood, Specific-Primer Directed DNA Sequence Analysis Using Automated Four-Color Fluorescence Detection and Labeled Primers. J.A. Luckey, H. Drossman, T. Kostichka, and L.M. Smith, High Speed DNA Sequencing by Capillary Gel Electrophoresis. V. Smith, M. Craxton, A.T. Bankier, C.M. Brown, W.D. Rawlinson, M.S. Chee, and B.G. Barrell, Microtiter Methods for Preparation and Fluorescent Sequencing of M13 Clones. P. Richterich and G.M. Church, DNA Sequencing with Direct Transfer Electrophoresis and Nonradioactive Detection. B.L. Iverson and P.B. Dervan, Adenine Specific Chemical DNA Sequencing Reaction. J. Kraus and T. Tahara, Direct DNA Sequencing of PCR Amplified Genomic DNA by Maxam-Gilbert Method. D.L. Steffens and R.W. Gross, Direct Sequencing of gggt11 Clones. T.L. McGuigan, K.J. Livak, and S. Brenner, DNA Fingerprinting by Sampled Sequencing. B.R. Krishnan, D. Kersulyte, I. Brikun, H.V. Huang, C.M. Berg, and D.E. Berg, Transposon- and PCR-Based Sequencing of DNAs cloned in ~gl Phage. C.M. Berg, G. Wang, L.D. Strausbaugh, and D.E. Berg, Transposon-Facilitated Sequencing of DNAs Cloned in Plasmids. PCR for Amplifying and Manipulating DNA: H. Ochman, F.J. Ayala, and D.L. Hartl, Use of Polymerase Chain Reaction to Amplify Segments outside Boundaries of Known Sequences. M.J. Geiger, M. Bull, D.D. Eckels, and J. Gorski, Modified Method for "One-Way PCR": Amplification of cDNA from mRNA with Unknown 5' Ends. D.M. Dorfman, Amplification of Bacteriophage Library Inserts Using Polymerase Chain Reaction (PCR). M. Frohman, Thermal RACE (Rapid Amplification of cDNA Ends). V. Jung and S. Pestka, Efficient Cloning of PCR-Generated DNA Containing Terminal Restriction Endonuclease Recognition Sites. G.S. Sandhu, J.W. Precup, and B.C. Kline, Rapid One-Step Characterization of Recombinant DNA Vectors by PCR Analysis of Whole Cells. E. Kawasaki, R. Saiki, and H. Erlich, Genetic Analysis Using PCR-Amplified DNA and Immobilized Oligonucleotide Probes: Reverse Dot Blot Typing. G. Sarkar and S.S. Sommer, Removal of DNA Contamination in PCR Reagents by UV Irradiation. C.D.K. Bottema, G. Sarkar, J.D. Cassady, S. Li, C.M. Dutton, and S.S. Sommer, PCR Amplification of Specific Alleles: General Method of Rapidly Detecting Mutations, Polymorphisms, and Haplotypes. C.A. Dionne and M. Jaye, Chromosome Assignment by PCR Techniques. M.D. Jones, Reverse Transcription of mRNA by Thermus aquaticus DNA Polymerase Followed by PCR Amplification. M. Becker-Andraae, Determination of Absolute Levels of mRNA by PCR Aided Transcript Titration Assay (PATTY). R.H. Scheuermann and S.R. Bauer, PCR-Based mRNA Quantification Using an Internal Standard: Method for Analysis of Oncogene Expression. A.-C. Syvadanen and H. Sadoderlund, Quantitation of Polymerase Chain Reaction Products by Affinity-Based Collection. Methods for Detecting DNA-Protein Interaction: M.G. Peterson and R. Tjian, Cross-Species PCR: Cloning of TATA Box Binding Proteins. O.S. Gabrielsen and J. Huet, Magnetic DNA Affinity Purification of a Yeast Transcription Factor. A.M. Lew, V.M. Marshall, and D.J. Kemp, Affinity Selection of PCR Products by DNA Binding Proteins. D.B. Tully and J.A. Cidlowski, Application of Protein Blotting Procedures to Evaluate Interactions of Steroid Receptors with DNA. H. Singh, Using Specific Recognition Site Probes to Identify Genes Coding for DNA Binding Proteins. P.B. Becker, F. Weih, and G. Sch~adutz, Footprinting of DNA-Binding Proteins in Intact Cells. M.J. Longley and D.W. Mosbaugh, In Situ Detection of DNA Metabolizing Enzymes Following Polyacrylamide Gel Electrophoresis. J.-C. Lelong, Simultaneous Characterization of DNA Binding Proteins and their Specific Genomic DNA Target Sites. Other Methods: C.T. Chung and R.H. Miller, One-Step Method for Preparation and Storage of Competent Escherichia coli Cells. P.A. Whittaker, Storage of Unamplified Phage Libraries on Nylon Filters. R. Padmanabhan, R. Padmanabhan, T. Howard, M.M. Gottesman, and B.H. Howard, Use of Magnetic Affinity Cell Sorting to Isolate Transiently Transfected Cells, Multidrug Resistant Cells, Somatic Cell Hybrids, and Virally Infected Cells. M. Yi and N. Ichikawa, and P.O.P. Ts'o, General Procedures for Detection of Genomic DNA Rearrangements at Restriction Fragment Level. Y.-R. Song, Z.-H. Ye, and J.E. Varner, Tissue Print Hybridization on Membrane for Localization of mRNA in Plant Tissue. G.I. Cassab, Localization of Cell Wall Proteins Using Tissue-Print Western Blot Technique. T.J. Guilfoyle, B.A. McClure, M.A. Gee, and G. Hagen, Tissue Print Hybridization: Simple Technique for Detecting RNA Directly. M.W. Mather, J.A. Keightley, and J. Fee, Recovery and Cloning of Genomic DNA Fragments from Dried Agarose Gels. J.G.K. Williams, M.K. Hanafey, J.A. Rafalski, and S.V. Tingey, Genetic Analysis Using RAPD Markers. Author Index. Subject Index.


The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Biochemists, biophysicists, physical chemists, and molecular and cell biologists.


No. of pages:
© Academic Press 1993
11th May 1993
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

Ray Wu

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.