The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Biochemists, biophysicists, physical chemists, and molecular and cell biologists.

Table of Contents

Methods for Sequencing DNA: U.B. Gyllensten and M. Allen, Methods for Sequencing of in Vitro Amplified DNA. L.F. Landweber and M. Kreitman, Strategy for Producing Single-Stranded DNA in Polymerase Chain Reaction for Direct Genomic Sequencing. J.G. Lawrence, D.L. Hartl, and H. Ochman, Sequencing Products of Polymerase Chain Reaction. R.L. Dorit, One-Sided PCR for Amplification and Sequencing cDNA. Z. Liu and P.B. Hackett, Reverse Cloning Procedure for Rapid Generation of Subclones for DNA Sequencing. W. Shen and M.M.Y. Waye, Simple Method for Generating Unidirectional Deletion Mutants for DNA Sequencing and Protein Engineering. K. Kretz and J.S. O'Brien, Direct Sequencing of PCR Products from Low-Melting Temperature Agarose. D.B. Olsen, G. Wunderlich, A. Uy, and F. Eckstein, Direct Sequencing of Polymerase Chain Reaction Products. J.P. Schofield, D.S.C. Jones, and M. Vaudin, Fluorescent and Radioactive Solid-Phase Dideoxy Sequencing of PCR Products in Microtiter Plates. Z. Du, L. Hood, and R.K. Wilson, Automated Fluorescent DNA Sequencing of PCR Products. R. Kaiser, T. Hunkapiller, C. Heiner, and L. Hood, Specific-Primer Directed DNA Sequence Analysis Using Automated Four-Color Fluorescence Detection and Labeled Primers. J.A. Luckey, H. Drossman, T. Kostichka, and L.M. Smith, High Speed DNA Sequencing by Capillary Gel Electrophoresis. V. Smith, M. Craxton, A.T. Bankier, C.M. Brown, W.D. Rawlinson, M.S. Chee, and B.G. Barrell, Microtiter Methods for Preparation and Fluorescent Sequencing of M13 Clones. P. Richterich and G.M. Church, DNA Sequencing with Direct Transfer Electrophoresis and Nonradioactive Detection. B.L. Ivers


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© 1993
Academic Press
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About the serial-volume-editor

Ray Wu

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY