Recombinant DNA, Part I, Volume 218

1st Edition

Serial Volume Editors: Ray Wu
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821197
eBook ISBN: 9780080883311
Imprint: Academic Press
Published Date: 11th May 1993
Page Count: 806


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Table of Contents

Methods for Sequencing DNA:

U.B. Gyllensten and M. Allen, Methods for Sequencing of in Vitro Amplified DNA.

L.F. Landweber and M. Kreitman, Strategy for Producing Single-Stranded DNA in Polymerase Chain Reaction for Direct Genomic Sequencing.

J.G. Lawrence, D.L. Hartl, and H. Ochman, Sequencing Products of Polymerase Chain Reaction.

R.L. Dorit, One-Sided PCR for Amplification and Sequencing cDNA.

Z. Liu and P.B. Hackett, Reverse Cloning Procedure for Rapid Generation of Subclones for DNA Sequencing.

W. Shen and M.M.Y. Waye, Simple Method for Generating Unidirectional Deletion Mutants for DNA Sequencing and Protein Engineering.

K. Kretz and J.S. O'Brien, Direct Sequencing of PCR Products from Low-Melting Temperature Agarose.

D.B. Olsen, G. Wunderlich, A. Uy, and F. Eckstein, Direct Sequencing of Polymerase Chain Reaction Products.

J.P. Schofield, D.S.C. Jones, and M. Vaudin, Fluorescent and Radioactive Solid-Phase Dideoxy Sequencing of PCR Products in Microtiter Plates.

Z. Du, L. Hood, and R.K. Wilson, Automated Fluorescent DNA Sequencing of PCR Products.

R. Kaiser, T. Hunkapiller, C. Heiner, and L. Hood, Specific-Primer Directed DNA Sequence Analysis Using Automated Four-Color Fluorescence Detection and Labeled Primers.

J.A. Luckey, H. Drossman, T. Kostichka, and L.M. Smith, High Speed DNA Sequencing by Capillary Gel Electrophoresis.

V. Smith, M. Craxton, A.T. Bankier, C.M. Brown, W.D. Rawlinson, M.S. Chee, and B.G. Barrell, Microtiter Methods for Preparation and Fluorescent Sequencing of M13 Clones.

P. Richterich and G.M. Church, DNA Sequencing with Direct Transfer Electrophoresis and Nonradioactive Detection.

B.L. Ivers


The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Biochemists, biophysicists, physical chemists, and molecular and cell biologists.


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About the Serial Volume Editors

Ray Wu Serial Volume Editor

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA