Recombinant DNA, Part H, Volume 217

1st Edition

Serial Volume Editors: Ray Wu
Hardcover ISBN: 9780121821180
eBook ISBN: 9780080883304
Imprint: Academic Press
Published Date: 31st March 1993
Page Count: 724

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Table of Contents

Vectors for Expressing Cloned Genes: G. Sczakiel and K. Maeda, Vector pPLEX for Expression of Nonfusion Polypeptides in Escherichia coli. H. Orum and L.K. Poulsen, In-Frame Gene Fusion. S. Udaka and H. Yamagata, High Level Secretion of Heterologous Proteins by Bacillus brevis. S.E. Kane and M.M. Gottesman, Use of Multidrug Resistance Gene in Mammalian Expression Vectors. A. Lieber, V. Sandig, W. Sommer, S. B~adahring, and M. Strauss, Stable High Level Gene Expression in Mammalian Cells by T7 Phage RNA Polymerase. R. T~adopfer, C. Maas, C. H~adoricke-Grandpierre, J. Schell, and H.-H. Steinbiss, Expression Vectors for High Level Gene Expression in Dicotyledonous and Monocotyledonous Plants. K.D. MacFerrin, L. Chen, M.P. Terranova, S.L. Schreiber, and G.L. Verdine, Overproduction of Proteins Using the Expression-Cassette Polymerase Chain Reaction. T.L. Pauls and M.W. Berchtold, Efficient cDNA Amplification and Expression Using PCR Technology. V.I. Baranov and A.S. Spirin, Gene Expression in a Cell-Free System on the Preparative Scale. B. Dasmahapatra, Cell-Free Expression Vector: Use of Insect Virus Translational Initiation Signal for in Vitro Gene Expression. C. Coleclough, Cell-Free Expression of Large Collections of cDNA Clones Using Positive Selection, ~gl Phage Vectors. Mutagenesis and Gene Disruption: B. Hofer and B. K~aduhlein, Site-Specific Mutagenesis in Plasmids: A Gapped Circle Method. D.B. Olsen, J.R. Sayers, and F. Eckstein, Site-Directed Mutagenesis of Single-Stranded and Double-Stranded DNA by the Phosphorothioate Approach. L.-J. Zhao, Q.X. Zhang, and R. Padmanabhan, PCR-Based Point Mutag

Description

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Readership

Biochemists, molecular biologists, geneticists, microbiologists, genetic engineers, biotechnologists, analytical chemists, clinical chemists, industrial researchers working on protein products, plant scientists involved in plant breeding and pathology, researchers interested in DNA probes or dianostic kits, and graduate students in these areas.


Details

No. of pages:
724
Language:
English
Copyright:
© Academic Press 1993
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883304
Hardcover ISBN:
9780121821180

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY


About the Serial Volume Editors

Ray Wu Serial Volume Editor

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.