Recombinant DNA, Part H, Volume 217

1st Edition

Serial Volume Editors: Ray Wu
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821180
eBook ISBN: 9780080883304
Imprint: Academic Press
Published Date: 31st March 1993
Page Count: 724


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Table of Contents

Vectors for Expressing Cloned Genes:

G. Sczakiel and K. Maeda, Vector pPLEX for Expression of Nonfusion Polypeptides in Escherichia coli.

H. Orum and L.K. Poulsen, In-Frame Gene Fusion.

S. Udaka and H. Yamagata, High Level Secretion of Heterologous Proteins by Bacillus brevis.

S.E. Kane and M.M. Gottesman, Use of Multidrug Resistance Gene in Mammalian Expression Vectors.

A. Lieber, V. Sandig, W. Sommer, S. B~adahring, and M. Strauss, Stable High Level Gene Expression in Mammalian Cells by T7 Phage RNA Polymerase.

R. T~adopfer, C. Maas, C. H~adoricke-Grandpierre, J. Schell, and H.-H. Steinbiss, Expression Vectors for High Level Gene Expression in Dicotyledonous and Monocotyledonous Plants.

K.D. MacFerrin, L. Chen, M.P. Terranova, S.L. Schreiber, and G.L. Verdine, Overproduction of Proteins Using the Expression-Cassette Polymerase Chain Reaction.

T.L. Pauls and M.W. Berchtold, Efficient cDNA Amplification and Expression Using PCR Technology.

V.I. Baranov and A.S. Spirin, Gene Expression in a Cell-Free System on the Preparative Scale.

B. Dasmahapatra, Cell-Free Expression Vector: Use of Insect Virus Translational Initiation Signal for in Vitro Gene Expression.

C. Coleclough, Cell-Free Expression of Large Collections of cDNA Clones Using Positive Selection, ~gl Phage Vectors.

Mutagenesis and Gene Disruption:

B. Hofer and B. K~aduhlein, Site-Specific Mutagenesis in Plasmids: A Gapped Circle Method.

D.B. Olsen, J.R. Sayers, and F. Eckstein, Site-Directed Mutagenesis of Single-Stranded and Double-Stranded DNA by the Phosphorothioate Approach.

L.-J. Zhao, Q.X. Zhang, and R. Padmanabhan, PCR-Based Point Mutag


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About the Serial Volume Editors

Ray Wu Serial Volume Editor

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA