Recombinant DNA, Part G, Volume 216

1st Edition

Serial Volume Editors: Ray Wu
Hardcover ISBN: 9780121821173
eBook ISBN: 9780080883298
Imprint: Academic Press
Published Date: 23rd November 1992
Page Count: 689

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Table of Contents

Isolation, Synthesis, and Detection of DNA and RNA: F. Guidet and P. Langridge, Megabase DNA Preparation from Plant Tissue. M. Koob and W. Szybalski, Preparing and Using Agarose Microbeads. S. Anant and K. Subramanian, Methods for Rapid Isolation of Low Molecular Weight DNA from Bacteria and Animal Cells. J.D. Harding, R.L. Bebee, and G. Gebeyehu, Rapid, Specific DNA Isolation Using Methidium-Spermine-Sepharose. R.P. Kandpal, D.C. Ward, and S.M. Weissman, Chromosome Fishing: An Affinity Capture Method for Selective Enrichment of Large Genomic DNA Fragments. L. Ulanovsky, Trapping Electrophoresis of End-Modified DNA in Polyacrylamide Gels. C.C. Lee and C.T. Caskey, Direct cDNA Cloning Using PCR. Y.L. Chiang, Direct cDNA Cloning and Screening of Mutants Using the Polymerase Chain Reaction. J. Eberwine, C. Spencer, K. Miyashiro, S. Mackler, and R. Finnell, cDNA Synthesis in Situ: Methods and Applications. Z. Cai, J.K. Pullen, R.M. Horton, and L.R. Pease, Specific Amplification of cDNA from Targeted Members of Multigene Families. H. Toyoda, Long Synthetic Oligonucleotide Probes for Gene Analysis. D.J. Kemp, Colorimetric Detection of PCR-Amplified DNA Segments Using DNA Binding Proteins. A.M. Lew and M. Panaccio, A New Technique for Preparation of DNA from Blood for PCR in a Microtiter Tray. F.F. Chehab, J. Wall, and Y.W. Kan, Amplification and Detection of Specific DNA Sequences with Fluorescent PCR Primers: Application to the ~gDF508 Mutation in Cystic Fibrosis. S. Beck, Nonradioactive Detection of DNA Using Dioxetane Chemiluminescence. H.C. Birnboim, Extraction of High Molecular Weigh

Description

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Readership

Biochemists, biophysicists, physical chemists, and molecular and cell biologists.


Details

No. of pages:
689
Language:
English
Copyright:
© Academic Press 1992
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883298
Hardcover ISBN:
9780121821173

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY


About the Serial Volume Editors

Ray Wu Serial Volume Editor

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.