Recombinant DNA, Part E, Volume 154

1st Edition

Serial Volume Editors: Ray Wu Lawrence Grossman
Hardcover ISBN: 9780121820558
eBook ISBN: 9780080882710
Imprint: Academic Press
Published Date: 11th December 1987
Page Count: 576

Institutional Access


Table of Contents

Methods for Cloning cDNA. H. Okayama, M. Kawaichi, M. Brownstein, F. Lee, T. Yokota, and K. Arai, High-Efficiency Cloning of Full-Length cDNA; Construction and Screening of cDNA Expression Libraries for Mammalian Cells. G. Heidecker and J. Messing, A Method for Cloning Full-Length cDNA in Plasmid Vectors. D.C. Alexander, An Efficient Vector*b1Primer cDNA Cloning System. C. Coleclough, Use of Primer*b1Restriction End Adapters in cDNA Cloning. Identification of Cloned Genes and Mapping of Genes. A.A. Reyes and R.B. Wallace, Mapping of RNA Using S1 Nuclease and Synthetic Oligonucleotides. C.G. Miyada and R.B. Wallace, Oligonucleotide Hybridization Techniques. M. Snyder, D. Sweetser, R.A. Young, and R.W. Davis, *glgt 11: Gene Isolation with Antibody Probes and Other Applications. M.R. Gray, G.P. Mazzara, P. Reddy, M. Rosbash, Searching for Clones with Open Reading Frames. G.M. Weinstock, Use of Open Reading Frame Expression Vectors. J.D. Boeke, J. Trueheart, G. Natsoulis, and G.R. Fink, 5-Flouroorotic Acid as a Selective Agent in Yeast Molecular Genetics. F.J. de Bruijn, Transposon Tn5 Mutagenesis to Map Genes. J.D. Noti, M.N. Jagadish, and A.A. Szalay, Site-Directed Tn5 and Transplacement Mutagenesis: Methods to Identify Symbiotic Nitrogen Fixation Genes in Slow-Growing Rhizobium. Chemical Synthesis and Analysis of Oligodeoxynucleotides. R. Frank, A. Meyerhans, K. Schwellnus, and H. Blvcker, Simultaneous Synthesis and Biological Applications of DNA Fragments: An Efficient and Complete Methodology. H.W. DjurhuusYMatthes, A. Staub, and P. Chambon, The Segmented Paper Method: DNA Synthesis and Mutagenesis by Rapid Microscale "Shotgun Gene Synthesis". M.H. Caruthers, A.D. Barone, S.L. Beaucage, D.R. Dodds, E.F. Fisher, L.J. McBride, M. Matteucci, Z. Stabinsky, and J.-Y. Tang, Chemical Synthesis of Deoxyoligonucleotides by the Phosphoramidite Method. S.J. Horvath, J.R. Firca, T. Hunkapiller, M.W. Hunkapiller, and L. Hood, An Automated DNA Synthesizer Employing Deoxynucleosi

Description

Recombinant DNA methods are powerful, revolutionary techniques for at least two reasons. First, they allow the isolation of single genes in large amounts from a pool of thousands or millions of genes. Second, the isolated genes or their regulatory regions can be modified at will and re-introduced into cells for expression at the RNA or protein levels. These attributes help to solve complex biological problems and to produce new and better products in the areas of health, agriculture and industry. Both volume 154 and 155 include the descriptions of several useful new restriction enzymes and rapid methods for DNA sequence analysis, along with a number of other useful methods.

Readership

Biochemists, geneticists, microbiologists, genetic engineers, analytical chemists, clinical chemists, molecular biologists, industrial researchers working on protein products, and plant scientists involved in plant breeding and plant pathology.


Details

No. of pages:
576
Language:
English
Copyright:
© Academic Press 1987
Published:
Imprint:
Academic Press
eBook ISBN:
9780080882710
Hardcover ISBN:
9780121820558

Reviews

@from:Praise for the Volume @qu:"This reviewer finds Methods in Enzymology Volume 154, Recombinant DNA Part E to be excellent reference book for persons working in molecular biology." @source:--ADOLESCENT AND PEDIATRIC GYNECOLOGY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY


About the Serial Volume Editors

Ray Wu Serial Volume Editor

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.

Lawrence Grossman Serial Volume Editor

Affiliations and Expertise

School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland, U.S.A.