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Recombinant DNA, Part D - 1st Edition - ISBN: 9780121820541, 9780080882703

Recombinant DNA, Part D, Volume 153

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: Ray Wu Lawrence Grossman
eBook ISBN: 9780080882703
Imprint: Academic Press
Published Date: 2nd December 1987
Page Count: 622


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Table of Contents

Vectors for Cloning DNA: Production of Single-Stranded Plasmid DNA. pKUN, Vectors for the Separate Production of Both DNA Strands of Recombinant Plasmids. Restriction Site Bank Vectors for Cloning in Gram-Negative Bacteria and Yeast. Plasmids for the Selection and Analysis of Prokaryotic Promoters. A *gl DNA Protocol Based on Purification of Phage on DEAE-Cellulose. Double cos Site Vectors: Simplified Cosmid Cloning. Improved in Vitro Packaging of *gl DNA. *gl Phage Vectors--Embl Series. Plasmid and Phage Vectors for Gene Cloning and Analysis in Streptomyces. Cosmid Shuttle Vectors for Cloning and Analysis of Streptomyces DNA. Host-Vector Systems for Gene Cloning in Cyanobacteria. Genetic Engineering of the Cyanobacterial Chromosome. Conjugal Transfer of Plasmids to Cyanobacteria. Site-Directed Chromosomal Rearrangements in Yeast. Improved Vectors for Plant Transformation: Expression Cassette Vectors and New Selectable Markers. Vectors for Cloning in Plant Cells.Binary Ti Vectors for Plant Transformation and Promoter Analysis. Detection for Monocot Transformation via Agrobacterium tumefaciens. Direct Gene Transfer to Protoplasts of Dicotyledonous and Monocotyledonous Plants by a Number of Methods, Including Electroporation. Uptake of DNA and RNA into Cells Mediated by Electroporation. Electroporation of DNA and RNA into Plant Protoplasts. Cloning Vectors of Mitochondrial Origin for Eukaryotes. Vectors for Expression of Cloned Genes: Short Homopeptide Leader Sequences Enhanced Production of Human Proinsulin in Escherichia coli. Expression of Bovine Growth Hormone Derivatives in Escherichia coli and the Use of the Derivatives to Produce Natural Sequence Growth Hormone by Cathepsin C Cleavage. Expression of Eukaryotic Genes in Escherichia coli with a Synthetic Two-Cistron System. Expression of Heterologous Unfused Protein in Eshcerichia coli. Directing Ribosomes to a Single mRNA Species: A Method to Study Ribosomal RNA Mutations and Their Effects on Translation of a Single Messenger in Escherichia coli. New Expression Vectors for Identifying and Testing Signal Structures for Initiation and Termination of Transcription. Synthesis and Sequence-Specific Proteolysis of Hybrid Proteins Produced in Escherichia coli. Expression Plasmid Containing the *gl PL Promoter and cI857 Repressor. Expression and Secretion of Foreign Proteins in Escherichia coli. Engineering for Protein Secretion in Gram-Positive Bacteria. Expression and Secretion Vectors for Yeast. Vaccinia Virus as an Expression Vector. Author Index. Subject Index.


The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, molecular biologists, microbiologists, geneticists, analytical chemists, clinical chemists; industrial researchers working on protein products; plant breeders and plant pathologists.


No. of pages:
© Academic Press 1987
2nd December 1987
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Ray Wu

Affiliations and Expertise

Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.

Lawrence Grossman

Affiliations and Expertise

School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland, U.S.A.